Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,576 G→T 12.5% intergenic (‑166/‑145) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5760GT12.5% 179.5 / 15.5 64intergenic (‑166/‑145)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base T (5/3);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

GAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGA  >  NC_002947/1639428‑1639721
                                                                                                                                                    |                                                                                                                                                 
gAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCcgcg                                                                                                                                                   >  6:73074/1‑149 (MQ=255)
 aaaGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCgcgc                                                                                                                                                  >  6:226190/1‑149 (MQ=255)
 aaaGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCgcgc                                                                                                                                                  <  5:388188/149‑1 (MQ=255)
 aaaGGCGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCgcgc                                                                                                                                                  <  5:18988/149‑1 (MQ=255)
      ggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTa                                                                                                                                             <  5:73074/149‑1 (MQ=255)
       gACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAg                                                                                                                                            <  5:226190/149‑1 (MQ=255)
        aCGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAgg                                                                                                                                           <  1:437958/149‑1 (MQ=255)
                tGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                     >  5:416511/1‑147 (MQ=255)
                tGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                     <  6:416511/147‑1 (MQ=255)
                 gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGt                                                                                                                                    <  7:146353/147‑1 (MQ=255)
                  aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTa                                                                                                                                 <  8:185977/149‑1 (MQ=255)
                    ccTGTAGTGACATCATGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATACCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                               <  5:238586/149‑1 (MQ=255)
                     cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTActc                                                                                                                              <  8:76230/149‑3 (MQ=38)
                     cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAccc                                                                                                                              >  3:265470/1‑149 (MQ=255)
                        tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcgc                                                                                                                            <  7:256816/148‑1 (MQ=37)
                          gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGa                                                                                                                          <  1:68190/148‑1 (MQ=255)
                          gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGa                                                                                                                          <  1:139512/148‑1 (MQ=255)
                             aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                                      <  8:344510/149‑1 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                     <  5:20049/132‑1 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                                     >  6:20049/1‑132 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                               >  6:215349/1‑138 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                               <  5:215349/138‑1 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                    >  6:154418/1‑149 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                    <  6:196198/149‑1 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                    <  5:154418/149‑1 (MQ=255)
                               aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                    >  1:191988/1‑149 (MQ=255)
                                tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAaga                                                                                                                       >  1:179100/1‑145 (MQ=25)
                                tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAaga                                                                                                                       <  2:179100/145‑1 (MQ=25)
                                tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                                   <  8:406553/149‑1 (MQ=255)
                                    ggTCACGCGCCCAATTGTTACGGATTTAAAAAAGGCCTGCGGTGCTTATAACGTTTTTTTATTTCTGCTGTTATGTGGCTGTCTGTTGGGGCCTCTTCGCTAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTATGAGGccc                                                                                                                  <  2:191988/146‑1 (MQ=255)
                                     gTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAAc                                                                                                              <  7:255143/149‑1 (MQ=255)
                                       cAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAg                                                                                                            >  5:177180/1‑149 (MQ=255)
                                          gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCAGAACAGTc                                                                                                          >  5:364375/1‑148 (MQ=255)
                                             ccccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCcaca                                                                                                      >  2:275148/1‑149 (MQ=255)
                                              cccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCcacac                                                                                                     <  6:177180/149‑1 (MQ=255)
                                                 aaTGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAg                                                                                                  <  1:275148/149‑1 (MQ=255)
                                                  aTGTTACGGCATGAAAAAAGCCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAgg                                                                                                 <  7:180630/149‑1 (MQ=255)
                                                      tACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGCGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGCACAGTCCACACAAGGCca                                                                                              >  6:278044/1‑148 (MQ=255)
                                                             tGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTCTCTGTTCGGGACTCTTCCCGAGTAAACCCGCGCCTGTAGGATCGGGTTTACCCGCGAAGAGGCCCGCACAGGCCACACAAGGCCACATTGCAg                                                                                      >  5:307084/1‑149 (MQ=39)
                                                                  aaaGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAATCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTg                                                                                 <  1:354849/149‑1 (MQ=255)
                                                                    aGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTa                                                                               <  6:364375/149‑1 (MQ=255)
                                                                              tGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGCAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCt                                                                     <  7:94558/149‑1 (MQ=255)
                                                                                       cGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAATCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGcc                                                            >  2:403889/1‑149 (MQ=255)
                                                                                                                ggCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCGGTAGGAACGGGTTTACCCGCCAAGAGGCCCGAACAGTCCACACAAGGCCACATTGGAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCCCAAGGATGGc                                   >  8:178757/1‑149 (MQ=255)
                                                                                                                     tctgtGCGGGCCTCTGCCCCAGAAACCCCGCGCCGGTAGCACCGGGTTAACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                              <  5:278044/149‑1 (MQ=255)
                                                                                                                        gtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                            >  8:256342/1‑148 (MQ=255)
                                                                                                                         ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGGCTGa                          >  2:240866/1‑149 (MQ=255)
                                                                                                                           cGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCTAGCCCTCGCTAGGATGGCCGTGTCTGAt                         <  6:147546/148‑1 (MQ=255)
                                                                                                                           cGGCCCTCTTCGCGAGAAACCCCGCGCCTTTAGCAACGGTTTTACCCGCGAAGTGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGAt                         <  1:424860/148‑1 (MQ=255)
                                                                                                                            gggCCTCTTCGCTAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATc                        <  1:99300/148‑1 (MQ=255)
                                                                                                                            gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGAAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg                       >  8:389871/1‑149 (MQ=255)
                                                                                                                              gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGt                     >  4:184283/1‑149 (MQ=255)
                                                                                                                                ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAGGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTc                             >  3:373778/1‑139 (MQ=255)
                                                                                                                                ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACACGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTc                             <  4:373778/139‑1 (MQ=255)
                                                                                                                                 tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttat                  >  1:70946/1‑149 (MQ=255)
                                                                                                                                   ttCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttatta                >  5:207976/1‑149 (MQ=255)
                                                                                                                                   ttCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttat                  <  7:389871/147‑1 (MQ=255)
                                                                                                                                      gcgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTAc               >  6:255638/1‑147 (MQ=255)
                                                                                                                                       cgggTAAACCCGTTCCTACAGGCGCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg            >  4:299784/4‑149 (MQ=37)
                                                                                                                                        gggTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg           >  8:218217/3‑149 (MQ=37)
                                                                                                                                        gggTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg           >  5:31457/3‑149 (MQ=37)
                                                                                                                                        gAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg           >  6:160346/1‑149 (MQ=255)
                                                                                                                                           tAAACCCGTTCCTACAGGCGCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGTCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCa         >  4:181556/1‑148 (MQ=38)
                                                                                                                                             aaCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAATCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCaaa       <  1:403889/148‑1 (MQ=255)
                                                                                                                                                ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAacac    >  7:67869/1‑148 (MQ=255)
                                                                                                                                                ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAacac    >  1:369838/1‑148 (MQ=255)
                                                                                                                                                ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAacac    >  8:259832/1‑148 (MQ=255)
                                                                                                                                                ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACg   >  8:159284/1‑149 (MQ=255)
                                                                                                                                                 cgcgCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGACAAACACGa  <  7:256342/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGA  >  NC_002947/1639428‑1639721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑