Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,693,686	C→G	7.3%	P52R (CCT→CGT) ‡	PP_1489 →	CheW domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,693,686	0	C	G	7.3%	210.8 / 5.3	68	P52R (CCT→CGT) ‡	PP_1489	CheW domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (34/29);  new base G (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

TTGCAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCC  >  NC_002947/1693541‑1693832                                                                                                                                                   |                                                                                                                                                    ttGCAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAcctgc                                                                                                                                                  >  8:54591/1‑149 (MQ=255) ttGCAACTGCGCACCGCTCGAGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAcctgc                                                                                                                                                  >  7:291116/1‑149 (MQ=255)  tGCAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAcctgcc                                                                                                                                                 >  7:194753/1‑149 (MQ=255)   gCAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAcctgcct                                                                                                                                                >  4:276112/1‑149 (MQ=255)    cAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAcctgcctg                                                                                                                                               <  1:406741/149‑1 (MQ=255)     aaCTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTgg                                                                                                                                              >  7:194235/1‑149 (MQ=255)     aaCTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTgg                                                                                                                                              >  4:137489/1‑149 (MQ=255)      aCTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAGGTGCCTggg                                                                                                                                             <  5:360172/149‑1 (MQ=255)       cTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCTGCTCAAGCCGATCGCCCAGGCAGGTGCCTgggc                                                                                                                                            <  4:118145/149‑2 (MQ=255)       cTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCAGGTGCCTgggc                                                                                                                                            <  1:269600/149‑2 (MQ=255)            cACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCgg                                                                                                                                       <  1:55079/149‑1 (MQ=255)                      gcggcgAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑c                                                                                                                            <  5:332446/149‑1 (MQ=255)                      gcggcgAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑c                                                                                                                            <  3:168087/149‑1 (MQ=255)                          cgAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCAt                                                                                                                        >  4:315012/1‑149 (MQ=255)                              cacCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTg                                                                                                                    <  3:137489/149‑1 (MQ=255)                                     ggggCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCt                                                                                                             <  6:387249/149‑1 (MQ=255)                                          gTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTc                                                                                                        <  8:291116/149‑1 (MQ=255)                                           ttGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTcc                                                                                                       >  1:90532/1‑149 (MQ=255)                                             gTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCgg                                                                                                     >  7:109462/1‑149 (MQ=255)                                              tACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCgg                                                                                                     <  8:109462/148‑1 (MQ=255)                                                ccTGCAGTTTCGCATCAGAGCGCAGCGGTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTc                                                                                                   <  4:291122/148‑1 (MQ=255)                                                 cTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCAt                                                                                                 <  8:194753/149‑1 (MQ=255)                                                     aGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGAc                                                                                             >  1:380077/1‑149 (MQ=255)                                                       tttCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGCTCCGGTCATCAACCt                                                                                           >  1:237077/1‑149 (MQ=255)                                                           gCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTgc                                                                                        <  8:194235/148‑1 (MQ=255)                                                              tCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTgccgcc                                                                                    <  3:315012/149‑1 (MQ=255)                                                                 gagCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCtt                                                                                  >  6:196764/1‑148 (MQ=255)                                                                   gcgcAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGa                                                                                <  1:90682/149‑1 (MQ=255)                                                                   gcgcAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCAGCCTTGAg                                                                               >  5:144475/1‑149 (MQ=255)                                                                       aGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCttt                                                                           >  5:206798/1‑149 (MQ=255)                                                                       aGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCCGGTTCCGGTCATCGACCTTGCCGCCTTGAGCttt                                                                           >  7:241566/1‑149 (MQ=255)                                                                        gCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTg                                                                          <  5:40557/149‑1 (MQ=255)                                                                                 ccTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCcgg                                                                 >  8:262401/1‑149 (MQ=255)                                                                                  cagAGCCTGAACGAGGTGATCGAAGTCTTGCCGCGTC‑AGCCGCTCAAGCCGATCCCCCACGCACCTGCCTGGGACGCGGGCATTCTCC‑CCAAACATGGCAAGCTCGTTCCGGTCAACGACCTTTCCGCATTGAGCTTTGGCAAGAcggc                                                                >  6:428528/3‑149 (MQ=255)                                                                                     aGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCcggcgg                                                              <  4:176178/148‑1 (MQ=255)                                                                                     aGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCcggcg                                                               <  7:54591/147‑1 (MQ=255)                                                                                       cGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCATTGAGCTTTGGCCAGCCGGCGGTg                                                            >  6:316682/1‑148 (MQ=255)                                                                                           cACGAGGTGATCGAAGTGTTTCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTTCAAc                                                        <  2:314588/148‑1 (MQ=255)                                                                                            aCGAGGTGATCGAAGTGTTGCCGCGTC‑AGCTGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACg                                                       >  4:190885/1‑148 (MQ=255)                                                                                            aCGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACg                                                       >  3:417928/1‑148 (MQ=255)                                                                                              gAGGTGATCGAAGTGTTGCCGCGTCAAGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCa                                                     >  1:4971/1‑149 (MQ=255)                                                                                               aGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCgg                                                                                                                                       <  7:112628/66‑1 (MQ=255)                                                                                               aGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCgg                                                                                                                                       >  8:112628/1‑66 (MQ=255)                                                                                                 gTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAg                                                 >  8:270820/1‑149 (MQ=255)                                                                                                    aTCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCAc                                              >  8:288086/1‑149 (MQ=255)                                                                                                        aaGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGc                                          >  4:275891/1‑149 (MQ=255)                                                                                                           tgtTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCctg                                       <  3:276112/149‑1 (MQ=255)                                                                                                           tgtTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCctg                                       <  7:270820/149‑1 (MQ=255)                                                                                                             ttGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGGTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCctggt                                     <  7:369623/149‑1 (MQ=255)                                                                                                               gCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCctggtgc                                   >  3:57795/1‑149 (MQ=255)                                                                                                                ccccgTC‑ACACGCTCAAGCCGATCGCCCAGGCAACTGCCTGGCTCGATGGCATTCTCG‑CCCAAAGTAGCAAGATGGTACCGGTCATCGACCTAGCCGCCATGAGCTTTGGCCACCCCGCCGTGCAACACACCAGCACCCGCcaggtgct                                  >  6:218302/4‑149 (MQ=255)                                                                                                                    gTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCCGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCC‑GCC‑TCGGT‑‑AGTGCACCAGCACCAGGCgggtgctggtgcgtt                          <  8:241566/149‑4 (MQ=25)                                                                                                                      c‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCa                            >  4:366993/1‑149 (MQ=255)                                                                                                                      c‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCa                            >  1:212434/1‑149 (MQ=255)                                                                                                                        aGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCAc                           >  5:118918/1‑149 (MQ=255)                                                                                                                         gCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCAc                           >  6:7915/1‑148 (MQ=255)                                                                                                                         gCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCAc                           >  3:46559/1‑148 (MQ=255)                                                                                                                            gCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTAcc                       >  2:82032/1‑149 (MQ=255)                                                                                                                              tCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTAcc                       >  8:303107/1‑147 (MQ=255)                                                                                                                                  gCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAggcg                 <  5:316682/149‑1 (MQ=255)                                                                                                                                     gATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGcc              >  3:101984/1‑149 (MQ=255)                                                                                                                                      aTCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCt             <  6:403753/149‑1 (MQ=255)                                                                                                                                        gacccAGGCAGGTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTg            >  1:225128/3‑148 (MQ=255)                                                                                                                                        gacccAGGCAGGTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTg            >  1:418575/3‑148 (MQ=255)                                                                                                                                          cccAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAg         >  3:226620/1‑149 (MQ=255)                                                                                                                                          cccAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAg         >  8:258459/1‑149 (MQ=255)                                                                                                                                          cccAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAg         <  4:101984/149‑1 (MQ=255)                                                                                                                                           ccAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGc        >  6:173229/1‑149 (MQ=255)                                                                                                                                             aGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCtt      <  5:7915/149‑1 (MQ=255)                                                                                                                                              ggCCCCTGCCTGGGTCGCGGCCATTCTCG‑CCCATCGTGGCCAGCGGGTGCCGGTCATCGCCCTTGCCGCCTTGCGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTg     <  8:362418/149‑1 (MQ=255)                                                                                                                                              ggCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTg     <  2:380077/149‑1 (MQ=255)                                                                                                                                                cACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGGCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCTACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGc   <  5:428528/149‑1 (MQ=255)                                                                                                                                                 aCCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGcc  <  4:46559/149‑1 (MQ=255)                                                                                                                                                   ctgcctgGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCa                            >  5:166946/1‑121 (MQ=255)                                                                                                                                                   ctgcctgGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCa                            <  6:166946/121‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TTGCAACTGCGCACCGCTCGGGCGGCGAACACCGAGGGGGCGTTGTACCTGCAGTTTCGCATCAGAGCGCAGCGTTTTGCCCTGAGCGTGCACGAGGTGATCGAAGTGTTGCCGCGTC‑AGCCGCTCAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCG‑CCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCC  >  NC_002947/1693541‑1693832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑