Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,713,747	T→A	5.8%	intergenic (+16/‑86)	tsaB → / → PP_1508	endopeptidase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,713,747	0	T	A	5.8%	207.7 / 2.3	69	intergenic (+16/‑86)	tsaB/PP_1508	endopeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/30);  new base A (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATCGCAGTCCTA  >  NC_002947/1713604‑1713894                                                                                                                                                 |                                                                                                                                                    ccTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCg                                                                                                                                                 <  6:222684/148‑1 (MQ=255)  cTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCCCG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGcc                                                                                                                                               >  5:327433/1‑149 (MQ=255)  cTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGcc                                                                                                                                               >  7:188943/1‑149 (MQ=255)       ccAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGGCGATAATCGACGAaca                                                                                                                                          <  3:293406/149‑1 (MQ=255)        cAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAacac                                                                                                                                         >  6:98424/1‑149 (MQ=255)         aaTGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACg                                                                                                                                        <  6:277919/149‑1 (MQ=255)         aaTGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACg                                                                                                                                        >  4:415697/1‑149 (MQ=255)            ccgctGGACCATCTCAGCCGCGCCCCCGTTGCCTGCGCCCGTGGAGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTa                                                                                                                                     <  4:336408/146‑1 (MQ=255)             ctctGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGGCGATAATCGACGAacagcact                                                                                                                                     <  7:286364/148‑6 (MQ=255)              tctGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAaca                                                                                                                                          >  8:356809/1‑142 (MQ=255)              tctGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAaca                                                                                                                                          <  7:356809/142‑1 (MQ=255)              tctGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTaa                                                                                                                                    >  7:362926/1‑148 (MQ=255)                  gACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAAcc                                                                                                                                <  3:397180/148‑1 (MQ=255)                   aCATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCtt                                                                                                                              <  5:380038/149‑1 (MQ=255)                    cATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCttt                                                                                                                             <  1:215339/149‑1 (MQ=255)                        ctcAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTgcg                                                                                                                         >  3:341110/1‑149 (MQ=255)                        ctcAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTgcg                                                                                                                         >  1:293553/1‑149 (MQ=255)                         tcAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTgcgc                                                                                                                        >  1:315808/1‑149 (MQ=255)                         tcAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTgcgc                                                                                                                        >  1:298359/1‑149 (MQ=255)                          cAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTgcgc                                                                                                                        >  4:116125/1‑148 (MQ=255)                                 gCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCtgtg                                                                                                                <  4:218181/149‑1 (MQ=255)                                    aCCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTc                                                                                                             <  2:415084/149‑1 (MQ=255)                                         ggCCTGGGGGCGTGGCGAGGGAAGCGTGGCCGAGCAGGCGCATGAGTTTTATCTGCGTGATAATGTAGCCTCT‑CCGATGATGCTCTGAGTGGTGTTCGTTGATTATCGGCATTCTGTCTATAACCTTTTGCGCGATCTGTGGTCCa                                                                                                           <  4:54417/145‑1 (MQ=255)                                          gCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGtt                                                                                                        <  3:116125/148‑1 (MQ=255)                                           ccTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTAt                                                                                                      <  2:298359/149‑1 (MQ=255)                                                  ccGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCaa                                                                                               <  3:91455/149‑1 (MQ=255)                                                   cGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAg                                                                                              <  4:341110/149‑1 (MQ=255)                                                     tGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCa                                                                                            >  1:144040/1‑149 (MQ=255)                                                         gAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAg                                                                                        <  2:290456/149‑1 (MQ=255)                                                          aGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGc                                                                                       >  5:134587/1‑149 (MQ=255)                                                              aaTCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACg                                                                                   >  6:89590/1‑149 (MQ=255)                                                               aTCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACgg                                                                                  <  3:415697/149‑1 (MQ=255)                                                                     gCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAActct                                                                            >  6:163625/1‑149 (MQ=255)                                                                     gCCGAGCAGGCGCAACCGGTGTATCTGCGCGATAATGTAGCCACG‑CCCAATAAGCACTGAGTGCTGGTCGTCTATTATCGCCGAACACGATAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACAGAActc                                                                             <  1:225932/148‑1 (MQ=255)                                                                      ccGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACGCCCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAActct                                                                            >  1:308084/1‑149 (MQ=255)                                                                      ccGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAActct                                                                            >  7:412531/1‑148 (MQ=255)                                                                         aGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGAtg                                                                        >  3:358438/1‑149 (MQ=255)                                                                              gcgcAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGAtgctgc                                                                    >  6:282124/1‑148 (MQ=255)                                                                              gcgcAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGAGATCTTTGGTCCAGTTATCACTCAAGCATTAGCGAAGGAACTCTGAtgctgc                                                                    >  6:323106/1‑148 (MQ=255)                                                                                ggacccGGTTTATCTGCGCGATAATGTAGCGCGG‑CCCAAGACGCACTGAGTGCTGTACCTCGATTATCGCCGCACCCGCTAAAACCTGTTGCGCGCTCTGTGGGCCAGTTATCACTCAGGCATTACCTACGCACCTCGGATGCTGCTa                                                                  >  6:111979/5‑148 (MQ=255)                                                                                    ccGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTg                                                             <  6:134587/149‑1 (MQ=255)                                                                                      ggTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGcc                                                           <  2:144040/149‑1 (MQ=255)                                                                                       gTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCca                                                          >  5:310207/1‑149 (MQ=255)                                                                                        tttATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCca                                                          >  5:152816/1‑148 (MQ=255)                                                                                           aTCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCcatcat                                                      >  5:300/1‑149 (MQ=255)                                                                                            tCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCAtt                                                     >  2:367740/1‑149 (MQ=255)                                                                                                cgcgATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTg                                                    <  6:327433/146‑1 (MQ=255)                                                                                                          tAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTc                                       <  4:202328/149‑1 (MQ=255)                                                                                                            gCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTcat                                     <  2:293553/149‑1 (MQ=255)                                                                                                                    ccAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTcatgccatgc                              <  5:163625/149‑1 (MQ=255)                                                                                                                    ccAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCAATCATTGGTCCTGAGTGATcatgccat                                >  4:384674/1‑147 (MQ=255)                                                                                                                      aagaagCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACAAATTGCGCGATATGTGGTCCAGTTATCACTCAAGCATTAGAGACGGAACTCTGATGCTGCTAAATTGCCATCATTGCTCCTGAGTGCTCATGCCATGCg                             >  2:273110/1‑148 (MQ=255)                                                                                                                      aagaagCACTGAGTGCTGTTCGTCGATTATCGACGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTCGTCCTGAGTGCTCATGCCATGCg                             >  2:267007/1‑148 (MQ=255)                                                                                                                           gCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGa                       >  4:336999/1‑149 (MQ=255)                                                                                                                             aCTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACg                     >  6:307651/1‑149 (MQ=255)                                                                                                                                gAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTcatgccat                                <  6:337140/135‑1 (MQ=255)                                                                                                                                gAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTcatgccat                                >  5:337140/1‑135 (MQ=255)                                                                                                                                 aGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTc                                       <  5:283219/127‑1 (MQ=255)                                                                                                                                 aGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTc                                       >  6:283219/1‑127 (MQ=255)                                                                                                                                  gTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTtc                <  5:89590/149‑1 (MQ=255)                                                                                                                                     gtGTTCGGCGATAATCGACGAACACGCTAAACCCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATCCTGCTAAATTGCCATCATTGGTCCTGAGAGCACCTGCCATGCGTATCGAAGGATTATCa             >  5:434402/2‑149 (MQ=255)                                                                                                                                     gtGTTCGGCGATAATCGACGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCa             >  1:27635/2‑149 (MQ=255)                                                                                                                                     cTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCa             <  5:282124/149‑1 (MQ=255)                                                                                                                                      tGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCAt            <  3:336999/149‑1 (MQ=255)                                                                                                                                       gTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATc           >  2:82739/1‑149 (MQ=255)                                                                                                                                           gtcgATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATCGCa        <  5:307651/148‑1 (MQ=255)                                                                                                                                            tcgATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTc                                                 <  1:325161/106‑1 (MQ=255)                                                                                                                                            tcgATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTc                                                 >  2:325161/1‑106 (MQ=255)                                                                                                                                              gATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATCGCAGTcc    >  5:101467/1‑149 (MQ=255)                                                                                                                                                ttATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATCGCAGTCCTa  >  5:311564/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGAGGCAATCGTCGCCGAGCAGGCGCAACCGGTTTATCTGCGCGATAATGTAGCCACG‑CCCAAGAAGCACTGAGTGCTGTTCGTCGATTATCGCCGAACACGCTAAAACCTTTTGCGCGATCTGTGGTCCAGTTATCACTCAAGCATTAGCGACGGAACTCTGATGCTGCTAAATTGCCATCATTGGTCCTGAGTGCTCATGCCATGCGTATCGACGGTTTCTCATCGCAGTCCTA  >  NC_002947/1713604‑1713894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑