Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,889,358 C→A 5.2% L305L (CTG→CTT) ‡ PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,889,3580CA 5.2% 314.3 / 5.5 96L305L (CTG→CTT) ‡PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (40/51);  new base A (3/2);  total (43/53)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

Only 100 of 104 total aligned reads displayed.

CATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATT  >  NC_002947/1889211‑1889505
                                                                                                                                                      |                                                                                                                                                    
cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCTTGCCGGgcagca                                                                                                                                                     <  6:209689/149‑1 (MQ=255)
cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCCGgcagca                                                                                                                                                     >  3:131502/1‑149 (MQ=255)
cATGGTCGACAACGCCACGGCCTCGACGATCACCATG‑‑TGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGgcagcag                                                                                                                                                    >  2:141846/1‑148 (MQ=255)
 aTGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGgcagcag                                                                                                                                                    >  6:205555/1‑149 (MQ=255)
     tCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATc                                                                                                                                                <  4:76394/149‑1 (MQ=255)
       gACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTg                                                                                                                                              >  2:413171/1‑149 (MQ=255)
         cAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGcc                                                                                                                                            >  8:103189/1‑149 (MQ=255)
          aaCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAAATCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACCAGTCGGAG‑CTCACCGTGCCCGGCAGCAGCCTCTGccc                                                                                                                                           >  1:337836/1‑149 (MQ=255)
           aCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGATGCTGCTGCCCg                                                                                                                                          <  6:12644/149‑1 (MQ=255)
           aCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGGCGGAGAATCACCGTGCCGGGCAGCAGCATCTGccc                                                                                                                                           <  1:26126/149‑1 (MQ=255)
            cGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCTGGCAGCAGCATCTGCCCgg                                                                                                                                         <  4:131502/149‑1 (MQ=255)
                aCGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGATGCTGCTGCCCGGcacg                                                                                                                                     <  6:84651/149‑4 (MQ=255)
                 cGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACGGCGGATGAACGCGAGGAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGGAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCGGGCCAgg                                                                                                                                    <  4:410159/149‑1 (MQ=255)
                 cGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAgg                                                                                                                                    <  4:176462/149‑1 (MQ=255)
                        gacgaTCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCg                                                                                                                             >  3:344316/1‑149 (MQ=255)
                          cgaTCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                           <  3:13468/149‑1 (MQ=255)
                                ccATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGcaccac                                                                                                                      >  2:274023/1‑148 (MQ=255)
                                 cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                           >  2:357783/1‑142 (MQ=255)
                                 cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                           <  1:357783/142‑1 (MQ=255)
                                 cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACg                                                                                                                     >  6:276339/1‑148 (MQ=255)
                                 cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACg                                                                                                                     >  5:50401/1‑148 (MQ=255)
                                   tGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑c                                                                                                                 <  5:205555/149‑1 (MQ=255)
                                    gCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GTCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGaa                                                                                                                   >  5:7501/1‑147 (MQ=255)
                                      cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCGAGGGCAATCGGCACCACGAA‑Catc                                                                                                              <  1:274023/149‑3 (MQ=255)
                                      cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAgc                                                                                                              >  6:298286/1‑149 (MQ=255)
                                      cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAgc                                                                                                              <  1:250039/149‑1 (MQ=255)
                                        gTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAAt                                                                                                                               <  5:189233/131‑1 (MQ=255)
                                        gTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAAt                                                                                                                               >  6:189233/1‑131 (MQ=255)
                                         tAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGcgcg                                                                                                           >  1:93349/1‑149 (MQ=255)
                                           gccgccGAGGCACCGCCGATGGACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                         <  3:5279/149‑1 (MQ=255)
                                           gccgccGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                         <  2:318078/149‑1 (MQ=255)
                                           gccgccGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                         <  2:268386/149‑1 (MQ=255)
                                            ccgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                         >  2:184959/1‑148 (MQ=255)
                                              gccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGAACCACGAA‑CAGCGCGGCCAg                                                                                                      >  8:288596/1‑149 (MQ=255)
                                                 gtggCACCGCGTATTAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGCCTCCCTGGGCAGGCTGATGCCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGt                                                                                                     <  1:141846/147‑1 (MQ=255)
                                                    gCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAg                                                                                                <  2:93349/149‑1 (MQ=255)
                                                     cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                               <  6:90125/149‑1 (MQ=255)
                                                     cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                               >  8:275697/1‑149 (MQ=255)
                                                             aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                       <  5:444171/149‑1 (MQ=255)
                                                             aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                       <  5:276339/149‑1 (MQ=255)
                                                              tGAACGCCTGCTGGGTAATGCTCGGGTGCGCCTCG‑‑TCCATGGGCAGGATGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCATCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCggg                                                                                      <  6:372472/149‑1 (MQ=255)
                                                              gcgatctcagcagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                       <  3:222620/141‑1 (MQ=255)
                                                                  cGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GTCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCGGTGCCAGGTACATCGGGAATa                                                                                  <  6:7501/149‑1 (MQ=255)
                                                                   gCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATAc                                                                                 >  2:399669/1‑149 (MQ=255)
                                                                      agcagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGACGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                       >  4:222620/1‑140 (MQ=255)
                                                                        cagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgc                                                                            <  3:274413/149‑1 (MQ=255)
                                                                            gCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgcgcgc                                                                        >  5:15033/1‑149 (MQ=255)
                                                                             cAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGcc                                                                       <  1:184959/149‑1 (MQ=255)
                                                                                 gCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgc                                                                   <  7:145356/149‑1 (MQ=255)
                                                                                  cTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                  >  3:427729/1‑149 (MQ=255)
                                                                                     gggTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcgc                                                                 <  2:262019/147‑1 (MQ=255)
                                                                                     gggTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCaa                                                               <  7:105486/149‑1 (MQ=255)
                                                                                         gcgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTcc                                                           >  3:273039/1‑149 (MQ=255)
                                                                                          cgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCt                                                          <  4:344316/149‑1 (MQ=255)
                                                                                          cgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCt                                                          <  5:399522/149‑1 (MQ=255)
                                                                                           gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                  <  6:408982/140‑1 (MQ=255)
                                                                                           gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                  >  5:408982/1‑140 (MQ=255)
                                                                                           gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTg                                                         <  7:103189/149‑1 (MQ=255)
                                                                                            ccTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                        >  7:222107/1‑149 (MQ=255)
                                                                                            ccTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                        <  2:337836/149‑1 (MQ=255)
                                                                                             cTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCg                                                       >  6:112341/1‑149 (MQ=255)
                                                                                                g‑‑gCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCgg                                                      <  3:278694/147‑1 (MQ=255)
                                                                                                      aGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGAt                                                <  5:65712/149‑1 (MQ=255)
                                                                                                      aGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGAt                                                <  7:195392/149‑1 (MQ=255)
                                                                                                       ggggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATg                                               <  8:222107/149‑1 (MQ=255)
                                                                                                       ggggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCCTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCGATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATg                                               >  2:223820/1‑149 (MQ=255)
                                                                                                        gggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGt                                              >  8:121456/1‑149 (MQ=255)
                                                                                                          gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGGCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                            <  3:46014/149‑1 (MQ=255)
                                                                                                          gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                               >  8:273169/1‑98 (MQ=255)
                                                                                                          gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                               <  7:273169/98‑1 (MQ=255)
                                                                                                          gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                            <  5:298286/149‑1 (MQ=255)
                                                                                                          gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                            >  6:123801/1‑149 (MQ=255)
                                                                                                            aGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtctc                                          <  1:399669/149‑1 (MQ=255)
                                                                                                               cTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTccac                                       <  7:121456/149‑1 (MQ=255)
                                                                                                                tgatgaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTccacc                                      >  6:367940/1‑149 (MQ=255)
                                                                                                                    gaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                        <  1:408383/127‑1 (MQ=255)
                                                                                                                     acgaacgaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACgg                                  >  1:344451/1‑148 (MQ=255)
                                                                                                                        aacgaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAAc                              >  6:63112/1‑149 (MQ=255)
                                                                                                                           gaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTg                           <  6:50401/149‑1 (MQ=255)
                                                                                                                            aGTCGGAG‑ATCACCCTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCGATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTgg                          <  1:223820/149‑1 (MQ=255)
                                                                                                                              tCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGaa                        <  7:367943/149‑1 (MQ=255)
                                                                                                                                 gag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTg                     >  7:398789/1‑149 (MQ=255)
                                                                                                                                  ag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                    <  4:273039/149‑1 (MQ=255)
                                                                                                                                  ag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                    <  5:112341/149‑1 (MQ=255)
                                                                                                                                       cACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAATCAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgg                 >  1:325628/1‑149 (MQ=255)
                                                                                                                                        ccctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg               >  2:419455/6‑149 (MQ=255)
                                                                                                                                         cctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg               >  4:181896/5‑148 (MQ=255)
                                                                                                                                         ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg               >  6:32547/1‑148 (MQ=255)
                                                                                                                                          ctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg               >  6:55202/4‑147 (MQ=255)
                                                                                                                                            tgtcGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg           >  7:147938/4‑149 (MQ=255)
                                                                                                                                            tGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg           <  6:125576/149‑1 (MQ=255)
                                                                                                                                              ccGGTCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCTCCACGGTAACGTGGAACTGTCGGggcaggc          <  5:367940/148‑1 (MQ=255)
                                                                                                                                               cGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGgcaggcag        >  7:328235/1‑149 (MQ=255)
                                                                                                                                                gggCAGCAGCATCTGCCCGGCCAGTGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACg                                   <  3:41513/121‑1 (MQ=255)
                                                                                                                                                gggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTTACAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACg                                   >  4:41513/1‑121 (MQ=255)
                                                                                                                                                 ggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgat      <  2:344451/149‑1 (MQ=255)
                                                                                                                                                  gcagcagcaTCTGCCCGGCCATGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatg     <  1:413171/149‑1 (MQ=255)
                                                                                                                                                    agcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatgat   >  7:249466/1‑149 (MQ=255)
                                                                                                                                                    agcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatgat   >  4:259379/1‑149 (MQ=255)
                                                                                                                                                     gcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGAtt  <  7:408271/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATT  >  NC_002947/1889211‑1889505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑