Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,889,362 A→T 5.2% M304K (ATG→AAG)  PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,889,3620AT 5.2% 301.3 / 4.5 96M304K (ATG→AAG) PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (39/51);  new base T (3/2);  total (43/53)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 101 total aligned reads displayed.

TCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGCC  >  NC_002947/1889216‑1889508
                                                                                                                                                     |                                                                                                                                                   
tCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATc                                                                                                                                                   <  4:76394/149‑1 (MQ=255)
  gACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTg                                                                                                                                                 >  2:413171/1‑149 (MQ=255)
    cAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGcc                                                                                                                                               >  8:103189/1‑149 (MQ=255)
     aaCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAAATCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACCAGTCGGAG‑CTCACCGTGCCCGGCAGCAGCCTCTGccc                                                                                                                                              >  1:337836/1‑149 (MQ=255)
      aCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGATGCTGCTGCCCg                                                                                                                                             <  6:12644/149‑1 (MQ=255)
      aCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGGCGGAGAATCACCGTGCCGGGCAGCAGCATCTGccc                                                                                                                                              <  1:26126/149‑1 (MQ=255)
       cGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCTGGCAGCAGCATCTGCCCgg                                                                                                                                            <  4:131502/149‑1 (MQ=255)
        gCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGACGGGCAGCAGCATCTGCCCGGc                                                                                                                                           >  6:444171/1‑149 (MQ=255)
           aCGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGATGCTGCTGCCCGGcacg                                                                                                                                        <  6:84651/149‑4 (MQ=255)
            cGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACGGCGGATGAACGCGAGGAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGGAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCGGGCCAgg                                                                                                                                       <  4:410159/149‑1 (MQ=255)
            cGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAgg                                                                                                                                       <  4:176462/149‑1 (MQ=255)
                   gacgaTCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCg                                                                                                                                >  3:344316/1‑149 (MQ=255)
                     cgaTCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                              <  3:13468/149‑1 (MQ=255)
                           ccATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGcaccac                                                                                                                         >  2:274023/1‑148 (MQ=255)
                            cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                              >  2:357783/1‑142 (MQ=255)
                            cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGc                                                                                                                              <  1:357783/142‑1 (MQ=255)
                            cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACg                                                                                                                        >  6:276339/1‑148 (MQ=255)
                            cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACg                                                                                                                        >  5:50401/1‑148 (MQ=255)
                              tGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑c                                                                                                                    <  5:205555/149‑1 (MQ=255)
                               gCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GTCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGaa                                                                                                                      >  5:7501/1‑147 (MQ=255)
                                 cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCGAGGGCAATCGGCACCACGAA‑Catc                                                                                                                 <  1:274023/149‑3 (MQ=255)
                                 cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAgc                                                                                                                 >  6:298286/1‑149 (MQ=255)
                                 cGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAgc                                                                                                                 <  1:250039/149‑1 (MQ=255)
                                   gTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAAt                                                                                                                                  >  6:189233/1‑131 (MQ=255)
                                   gTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAAt                                                                                                                                  <  5:189233/131‑1 (MQ=255)
                                    tAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGcgcg                                                                                                              >  1:93349/1‑149 (MQ=255)
                                      gccgccGAGGCACCGCCGATGGACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                            <  3:5279/149‑1 (MQ=255)
                                      gccgccGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                            <  2:268386/149‑1 (MQ=255)
                                      gccgccGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                            <  2:318078/149‑1 (MQ=255)
                                       ccgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGc                                                                                                            >  2:184959/1‑148 (MQ=255)
                                         gccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGAACCACGAA‑CAGCGCGGCCAg                                                                                                         >  8:288596/1‑149 (MQ=255)
                                            gtggCACCGCGTATTAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGCCTCCCTGGGCAGGCTGATGCCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGt                                                                                                        <  1:141846/147‑1 (MQ=255)
                                               gCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAg                                                                                                   <  2:93349/149‑1 (MQ=255)
                                                cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                                  <  6:90125/149‑1 (MQ=255)
                                                cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                                  >  8:275697/1‑149 (MQ=255)
                                                        aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                          <  5:444171/149‑1 (MQ=255)
                                                        aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                          <  5:276339/149‑1 (MQ=255)
                                                         tGAACGCCTGCTGGGTAATGCTCGGGTGCGCCTCG‑‑TCCATGGGCAGGATGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCATCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCggg                                                                                         <  6:372472/149‑1 (MQ=255)
                                                         gcgatctcagcagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                          <  3:222620/141‑1 (MQ=255)
                                                             cGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GTCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCGGTGCCAGGTACATCGGGAATa                                                                                     <  6:7501/149‑1 (MQ=255)
                                                              gCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATAc                                                                                    >  2:399669/1‑149 (MQ=255)
                                                                 agcagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGACGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCgg                                                                                          >  4:222620/1‑140 (MQ=255)
                                                                   cagGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgc                                                                               <  3:274413/149‑1 (MQ=255)
                                                                       gCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgcgcgc                                                                           >  5:15033/1‑149 (MQ=255)
                                                                        cAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGcc                                                                          <  1:184959/149‑1 (MQ=255)
                                                                            gCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgc                                                                      <  7:145356/149‑1 (MQ=255)
                                                                             cTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                     >  3:427729/1‑149 (MQ=255)
                                                                                gggTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcgc                                                                    <  2:262019/147‑1 (MQ=255)
                                                                                gggTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCaa                                                                  <  7:105486/149‑1 (MQ=255)
                                                                                    gcgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTcc                                                              >  3:273039/1‑149 (MQ=255)
                                                                                     cgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCt                                                             <  5:399522/149‑1 (MQ=255)
                                                                                     cgcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCt                                                             <  4:344316/149‑1 (MQ=255)
                                                                                      gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                     <  6:408982/140‑1 (MQ=255)
                                                                                      gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGgcg                                                                     >  5:408982/1‑140 (MQ=255)
                                                                                      gcCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTg                                                            <  7:103189/149‑1 (MQ=255)
                                                                                       ccTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           >  7:222107/1‑149 (MQ=255)
                                                                                       ccTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           <  2:337836/149‑1 (MQ=255)
                                                                                        cTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCg                                                          >  6:112341/1‑149 (MQ=255)
                                                                                           g‑‑gCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCgg                                                         <  3:278694/147‑1 (MQ=255)
                                                                                                 aGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGAt                                                   <  7:195392/149‑1 (MQ=255)
                                                                                                 aGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGAt                                                   <  5:65712/149‑1 (MQ=255)
                                                                                                  ggggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATg                                                  <  8:222107/149‑1 (MQ=255)
                                                                                                  ggggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCCTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCGATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATg                                                  >  2:223820/1‑149 (MQ=255)
                                                                                                   gggCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGt                                                 >  8:121456/1‑149 (MQ=255)
                                                                                                     gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGGCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                               <  3:46014/149‑1 (MQ=255)
                                                                                                     gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                                  >  8:273169/1‑98 (MQ=255)
                                                                                                     gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAgg                                                                                                  <  7:273169/98‑1 (MQ=255)
                                                                                                     gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                               >  6:123801/1‑149 (MQ=255)
                                                                                                     gCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtc                                               <  5:298286/149‑1 (MQ=255)
                                                                                                       aGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtctc                                             <  1:399669/149‑1 (MQ=255)
                                                                                                          cTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTccac                                          <  7:121456/149‑1 (MQ=255)
                                                                                                           tgatgaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTccacc                                         >  6:367940/1‑149 (MQ=255)
                                                                                                              tgaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACg                                      >  5:125576/1‑149 (MQ=255)
                                                                                                               gaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           >  2:408383/1‑127 (MQ=255)
                                                                                                               gaCGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           <  1:408383/127‑1 (MQ=255)
                                                                                                                acgaacgaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACgg                                     >  1:344451/1‑148 (MQ=255)
                                                                                                                   aacgaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAAc                                 >  6:63112/1‑149 (MQ=255)
                                                                                                                      gaGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTg                              <  6:50401/149‑1 (MQ=255)
                                                                                                                       aGTCGGAG‑ATCACCCTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCGATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTgg                             <  1:223820/149‑1 (MQ=255)
                                                                                                                         tCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGaa                           <  7:367943/149‑1 (MQ=255)
                                                                                                                            gag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTg                        >  7:398789/1‑149 (MQ=255)
                                                                                                                             ag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                       <  4:273039/149‑1 (MQ=255)
                                                                                                                             ag‑aTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                       <  5:112341/149‑1 (MQ=255)
                                                                                                                                  cACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAATCAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgg                    >  1:325628/1‑149 (MQ=255)
                                                                                                                                   ccctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg                  >  2:419455/6‑149 (MQ=255)
                                                                                                                                    cctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg                  >  4:181896/5‑148 (MQ=255)
                                                                                                                                    ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg                  >  6:32547/1‑148 (MQ=255)
                                                                                                                                     ctggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCgggg                  >  6:55202/4‑147 (MQ=255)
                                                                                                                                       tgtcGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg              >  7:147938/4‑149 (MQ=255)
                                                                                                                                       tGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg              <  6:125576/149‑1 (MQ=255)
                                                                                                                                         ccGGTCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCTCCACGGTAACGTGGAACTGTCGGggcaggc             <  5:367940/148‑1 (MQ=255)
                                                                                                                                          cGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGgcaggcag           >  7:328235/1‑149 (MQ=255)
                                                                                                                                           gggCAGCAGCATCTGCCCGGCCAGTGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACg                                      <  3:41513/121‑1 (MQ=255)
                                                                                                                                           gggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTTACAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACg                                      >  4:41513/1‑121 (MQ=255)
                                                                                                                                            ggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgat         <  2:344451/149‑1 (MQ=255)
                                                                                                                                             gcagcagcaTCTGCCCGGCCATGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatg        <  1:413171/149‑1 (MQ=255)
                                                                                                                                               agcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatgat      >  7:249466/1‑149 (MQ=255)
                                                                                                                                               agcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgatgat      >  4:259379/1‑149 (MQ=255)
                                                                                                                                                gcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGAtt     <  7:408271/149‑1 (MQ=255)
                                                                                                                                                   gcaTCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGcc  <  5:63112/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCG‑‑GCCAGGGGCAGGCTGATGACGAACGAGTCGGAG‑ATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAA‑CAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGCC  >  NC_002947/1889216‑1889508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑