Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,898,647 T→C 6.6% intergenic (+53/‑118) PP_t16 → / → PP_t17 tRNA‑Asp/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,898,6470TC 6.6% 167.6 / 2.4 60intergenic (+53/‑118)PP_t16/PP_t17tRNA‑Asp/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base C (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

CACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGG  >  NC_002947/1898500‑1898783
                                                                                                                                                   |                                                                                                                                        
ctacacTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCtt                                                                                                                                         <  4:242014/146‑1 (MQ=17)
     cTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGt                                                                                                                                    >  6:433217/1‑149 (MQ=34)
       cGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCg                                                                                                                                  >  3:272308/1‑149 (MQ=34)
                 cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                        <  1:366290/149‑1 (MQ=35)
                    aGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAAAAAGCGACTCTAAGGTCGCTTTCTTtgttac                                                                                                                     <  3:369954/149‑4 (MQ=11)
                      cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCt                                                                                                                    >  6:391808/1‑148 (MQ=39)
                      cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCt                                                                                                                    >  4:236967/1‑148 (MQ=39)
                           gTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAtttt                                                                                                              <  7:317723/149‑1 (MQ=255)
                            ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAttttt                                                                                                             <  3:137315/149‑1 (MQ=255)
                            ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCAACCTTAGAGTCGCTTTTTTTGTGCCTGAttttt                                                                                                             <  8:133247/149‑1 (MQ=255)
                              cAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCTGATTTTTgg                                                                                                           >  2:317283/1‑149 (MQ=255)
                               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCtttt                                                                                                                             <  5:80802/130‑1 (MQ=35)
                               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCtttt                                                                                                                             >  6:80802/1‑130 (MQ=35)
                               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGGCGGATTTTTTgt                                                                                                          >  4:109273/1‑149 (MQ=255)
                                gTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGGTGCGCCCTATTCCACTTCCCGGGCCCACTGCACCCCCGGAAGTAACAAAGAAAGCGACCTGAGAGGCGCGTGGTTTGGGGCGGAGGTTTGgtg                                                                                                         >  4:279336/1‑149 (MQ=11)
                                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTg                                                                                                            >  3:162947/1‑139 (MQ=255)
                                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTg                                                                                                            <  4:162947/139‑1 (MQ=255)
                                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGAc                                                                                                   <  4:272308/148‑1 (MQ=255)
                                           ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTTTGTGAcagcag                                                                                              >  8:296203/3‑144 (MQ=17)
                                             ccGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgt                                                                                             <  5:433217/148‑1 (MQ=255)
                                              cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgtgt                                                                                           <  1:116792/149‑1 (MQ=255)
                                               ggCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGATTTTTTTTTGGGGGATTTTTGGTGGTTGTGCCAAgtgt                                                                                           >  8:170333/1‑148 (MQ=14)
                                                       aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACt                                                                                  >  6:226161/1‑149 (MQ=255)
                                                          ccGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCg                                                                               <  1:317283/149‑1 (MQ=255)
                                                           cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGa                                                                              <  2:330714/149‑1 (MQ=255)
                                                            gggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGAt                                                                             <  3:109273/149‑1 (MQ=255)
                                                                          cGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGGCGGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCa                                                                 >  3:312535/1‑147 (MQ=255)
                                                                           gAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTTCCTGATTTTTGGTGTTTGACCCAAGTGTGAAAAAAATTCGAAAATTGCTTGCCcgag                                                              >  8:25705/1‑149 (MQ=21)
                                                                           gAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAAATTCGATAATTTCTTGCCagag                                                              >  1:196423/1‑149 (MQ=255)
                                                                                ccGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGcc                                                          <  6:7315/148‑1 (MQ=255)
                                                                                ccGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGcc                                                          <  5:226161/148‑1 (MQ=255)
                                                                                 cGTCCGCTGCGCCATATTCCACTTCCATGGCCCACTCCACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCaa                                                        <  6:269077/149‑1 (MQ=255)
                                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                   <  5:391808/149‑1 (MQ=255)
                                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                   >  3:408121/1‑149 (MQ=255)
                                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                   >  7:385992/1‑149 (MQ=255)
                                                                                        tGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGACTGATTTTTGGTGTTTTACCGAAatgt                                                                                           >  6:27243/1‑107 (MQ=18)
                                                                                        tGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgtgt                                                                                           <  5:27243/107‑1 (MQ=255)
                                                                                          cgcCATATTCGACTTCCAGGGCCCTCTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGGCGCTTTTTTTGTGCCTGATTTTTGGCGTTTGCCCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGccc                                                 <  5:303359/147‑1 (MQ=255)
                                                                                          cgcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGccc                                                 <  8:385992/147‑1 (MQ=255)
                                                                                          cgcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAATTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGt                                               <  8:396941/149‑1 (MQ=255)
                                                                                           gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                              <  4:214065/149‑1 (MQ=255)
                                                                                                 ttCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgc                                        >  2:340715/1‑149 (MQ=255)
                                                                                                  tCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcg                                       >  3:335070/1‑149 (MQ=255)
                                                                                                   ccACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcg                                       <  3:236967/148‑1 (MQ=255)
                                                                                                     aCTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTAGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgcgc                                    >  4:232809/1‑149 (MQ=255)
                                                                                                             ggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCga                            <  3:279336/149‑1 (MQ=255)
                                                                                                              gCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgag                           <  2:354552/149‑1 (MQ=255)
                                                                                                               cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                          <  1:340715/149‑1 (MQ=255)
                                                                                                               cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                          <  4:408121/149‑1 (MQ=255)
                                                                                                                           ccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATTGGTGAt              >  3:172628/1‑149 (MQ=255)
                                                                                                                           ccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt              <  4:312535/149‑1 (MQ=255)
                                                                                                                              tcaggcaCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                   <  4:72336/135‑1 (MQ=38)
                                                                                                                              tcaggcaCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                   >  3:72336/7‑141 (MQ=38)
                                                                                                                              gAAGTAACAAAGAACGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg           <  7:296203/149‑1 (MQ=255)
                                                                                                                                  tAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCt         >  5:130573/1‑147 (MQ=255)
                                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAATAAACTTCGATTAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg   >  6:393289/1‑149 (MQ=255)
                                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCca                                  <  6:324605/118‑1 (MQ=255)
                                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCca                                  >  5:324605/1‑118 (MQ=255)
                                                                                                                                       aagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg  <  1:218617/149‑1 (MQ=255)
                                                                                                                                        aaaaagCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg  >  1:186277/3‑148 (MQ=38)
                                                                                                                                         gaaagCGCCCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg  >  7:127579/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
CACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGG  >  NC_002947/1898500‑1898783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑