Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,898,660	T→C	7.1%	intergenic (+66/‑105)	PP_t16 → / → PP_t17	tRNA‑Asp/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,898,660	0	T	C	7.1%	153.5 / 2.3	57	intergenic (+66/‑105)	PP_t16/PP_t17	tRNA‑Asp/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (22/30);  new base C (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCCTTACAAGCAGA  >  NC_002947/1898517‑1898807                                                                                                                                                |                                                                                                                                                    cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                                <  1:366290/149‑1 (MQ=35)    aGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAAAAAGCGACTCTAAGGTCGCTTTCTTtgttac                                                                                                                                             <  3:369954/149‑4 (MQ=11)      cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCt                                                                                                                                            >  6:391808/1‑148 (MQ=39)      cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCt                                                                                                                                            >  4:236967/1‑148 (MQ=39)           gTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAtttt                                                                                                                                      <  7:317723/149‑1 (MQ=255)            ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAttttt                                                                                                                                     <  3:137315/149‑1 (MQ=255)            ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCAACCTTAGAGTCGCTTTTTTTGTGCCTGAttttt                                                                                                                                     <  8:133247/149‑1 (MQ=255)              cAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCTGATTTTTgg                                                                                                                                   >  2:317283/1‑149 (MQ=255)               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCtttt                                                                                                                                                     <  5:80802/130‑1 (MQ=35)               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCtttt                                                                                                                                                     >  6:80802/1‑130 (MQ=35)               aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGGCGGATTTTTTgt                                                                                                                                  >  4:109273/1‑149 (MQ=255)                gTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGGTGCGCCCTATTCCACTTCCCGGGCCCACTGCACCCCCGGAAGTAACAAAGAAAGCGACCTGAGAGGCGCGTGGTTTGGGGCGGAGGTTTGgtg                                                                                                                                 >  4:279336/1‑149 (MQ=11)                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTg                                                                                                                                    >  3:162947/1‑139 (MQ=255)                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTg                                                                                                                                    <  4:162947/139‑1 (MQ=255)                       aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGAc                                                                                                                           <  4:272308/148‑1 (MQ=255)                           ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTTTGTGAcagcag                                                                                                                      >  8:296203/3‑144 (MQ=17)                             ccGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgt                                                                                                                     <  5:433217/148‑1 (MQ=255)                              cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgtgt                                                                                                                   <  1:116792/149‑1 (MQ=255)                               ggCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGATTTTTTTTTGGGGGATTTTTGGTGGTTGTGCCAAgtgt                                                                                                                   >  8:170333/1‑148 (MQ=14)                                       aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACt                                                                                                          >  6:226161/1‑149 (MQ=255)                                          ccGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCg                                                                                                       <  1:317283/149‑1 (MQ=255)                                           cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGa                                                                                                      <  2:330714/149‑1 (MQ=255)                                            gggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGAt                                                                                                     <  3:109273/149‑1 (MQ=255)                                                          cGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGGCGGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCa                                                                                         >  3:312535/1‑147 (MQ=255)                                                           gAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTTCCTGATTTTTGGTGTTTGACCCAAGTGTGAAAAAAATTCGAAAATTGCTTGCCcgag                                                                                      >  8:25705/1‑149 (MQ=21)                                                           gAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAAATTCGATAATTTCTTGCCagag                                                                                      >  1:196423/1‑149 (MQ=255)                                                                ccGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGcc                                                                                  <  6:7315/148‑1 (MQ=255)                                                                ccGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGcc                                                                                  <  5:226161/148‑1 (MQ=255)                                                                 cGTCCGCTGCGCCATATTCCACTTCCATGGCCCACTCCACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCaa                                                                                <  6:269077/149‑1 (MQ=255)                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                                           >  3:408121/1‑149 (MQ=255)                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                                           <  5:391808/149‑1 (MQ=255)                                                                      gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGc                                                                           >  7:385992/1‑149 (MQ=255)                                                                        tGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTTTGACTGATTTTTGGTGTTTTACCGAAatgt                                                                                                                   >  6:27243/1‑107 (MQ=18)                                                                        tGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgtgt                                                                                                                   <  5:27243/107‑1 (MQ=255)                                                                          cgcCATATTCGACTTCCAGGGCCCTCTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGGCGCTTTTTTTGTGCCTGATTTTTGGCGTTTGCCCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGccc                                                                         <  5:303359/147‑1 (MQ=255)                                                                          cgcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGccc                                                                         <  8:385992/147‑1 (MQ=255)                                                                          cgcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGAATTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGt                                                                       <  8:396941/149‑1 (MQ=255)                                                                           gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                                                      <  4:214065/149‑1 (MQ=255)                                                                                 ttCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgc                                                                >  2:340715/1‑149 (MQ=255)                                                                                  tCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcg                                                               >  3:335070/1‑149 (MQ=255)                                                                                   ccACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcg                                                               <  3:236967/148‑1 (MQ=255)                                                                                     aCTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTAGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgcgc                                                            >  4:232809/1‑149 (MQ=255)                                                                                             ggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCga                                                    <  3:279336/149‑1 (MQ=255)                                                                                              gCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgag                                                   <  2:354552/149‑1 (MQ=255)                                                                                               cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                                                  <  4:408121/149‑1 (MQ=255)                                                                                               cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                                                  <  1:340715/149‑1 (MQ=255)                                                                                                           ccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATTGGTGAt                                      >  3:172628/1‑149 (MQ=255)                                                                                                           ccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt                                      <  4:312535/149‑1 (MQ=255)                                                                                                              tcaggcaCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                                           <  4:72336/135‑1 (MQ=38)                                                                                                              tcaggcaCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                                           >  3:72336/7‑141 (MQ=38)                                                                                                              gAAGTAACAAAGAACGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg                                   <  7:296203/149‑1 (MQ=255)                                                                                                                  tAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCt                                 >  5:130573/1‑147 (MQ=255)                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAATAAACTTCGATTAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg                           >  6:393289/1‑149 (MQ=255)                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCca                                                          <  6:324605/118‑1 (MQ=255)                                                                                                                      aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCca                                                          >  5:324605/1‑118 (MQ=255)                                                                                                                       aagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg                          <  1:218617/149‑1 (MQ=255)                                                                                                                        aaaaagCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg                          >  1:186277/3‑148 (MQ=38)                                                                                                                         gaaagCGCCCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg                          >  7:127579/1‑147 (MQ=255)                                                                                                                                           gCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCCTTACaa       <  7:25705/148‑1 (MQ=255)                                                                                                                                                ttttGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCCTTACAAGCaga  <  8:127579/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    CGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAAAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCCTTACAAGCAGA  >  NC_002947/1898517‑1898807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑