Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,015,255 A→T 5.3% V78D (GTC→GAC)  PP_1794 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,015,2550AT 5.3% 228.4 / 2.5 75V78D (GTC→GAC) PP_1794hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/36);  new base T (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

CCGAGGTGTCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTC  >  NC_002947/2015109‑2015403
                                                                                                                                                  |                                                                                                                                                    
ccGAGGTGTCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTcgacg                                                                                                                                                    <  4:213430/149‑1 (MQ=255)
     gtgtCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGAc                                                                                                                                               <  3:63123/149‑1 (MQ=255)
           aCTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTc                                                                                                                                         <  1:105930/149‑1 (MQ=255)
           aCTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCATCGTCGACCTggct                                                                                                                                         >  5:377984/1‑147 (MQ=255)
               cAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGc                                                                                                                                       <  1:415587/147‑1 (MQ=255)
               cAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCGGCAGCAGCAGCCAGGTCGACGATGACCTGGTCGc                                                                                                                                       <  7:90921/147‑1 (MQ=255)
                aGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCATCGTCGACCTggctgctgc                                                                                                                                    <  6:377984/149‑8 (MQ=255)
                  ccAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCtt                                                                                                                                    >  8:316765/1‑147 (MQ=255)
                  ccAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCtt                                                                                                                                    >  8:104455/1‑147 (MQ=255)
                    aaGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAgc                                                                                                                                >  6:251894/1‑149 (MQ=255)
                        cAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCa                                                                                                                            >  1:107252/1‑149 (MQ=255)
                         aGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCAt                                                                                                                           <  3:336458/149‑1 (MQ=255)
                         aGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCAt                                                                                                                           <  7:328189/149‑1 (MQ=255)
                            gAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCGGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGa                                                                                                                        <  1:291430/149‑1 (MQ=255)
                                aCCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAg                                                                                                                     >  5:51859/1‑148 (MQ=255)
                                aCCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGt                                                                                                                    <  8:76439/149‑1 (MQ=255)
                                   tGGGCGTCAGCAGGTGCACCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCAGGTCGCCATTAGCGCCATCGACAGTAc                                                                                                                  >  4:264833/1‑148 (MQ=255)
                                      gCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCtt                                                                                                               >  8:84424/1‑148 (MQ=255)
                                         tCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAAc                                                                                                           <  3:334197/149‑1 (MQ=255)
                                          cagcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAAc                                                                                                           >  7:61437/1‑148 (MQ=255)
                                            gcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                        >  7:164458/1‑149 (MQ=255)
                                            gcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                        >  6:418808/1‑149 (MQ=255)
                                             cagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                        >  5:157142/1‑148 (MQ=255)
                                               ggTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCt                                                                                                     <  2:155673/149‑1 (MQ=255)
                                                 tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCATCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                   <  5:176130/149‑1 (MQ=255)
                                                 tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                   <  7:104455/149‑1 (MQ=255)
                                                 tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                   <  8:29238/149‑1 (MQ=255)
                                                     gCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGt                                                                                               <  4:162984/149‑1 (MQ=255)
                                                        ttGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGcc                                                                                            >  3:44365/1‑149 (MQ=255)
                                                            aGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAt                                                                                        <  7:20850/149‑1 (MQ=255)
                                                             gTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAtt                                                                                       <  3:115070/149‑1 (MQ=255)
                                                              ttACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTc                                                                                      >  5:257115/1‑149 (MQ=255)
                                                                aCCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCg                                                                                    >  5:308761/1‑149 (MQ=255)
                                                                 ccTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCgg                                                                                   <  6:51859/149‑1 (MQ=255)
                                                                 ccTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCg                                                                                    <  7:316765/148‑1 (MQ=255)
                                                                  cTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCgg                                                                                   >  1:22960/1‑148 (MQ=255)
                                                                   ttCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTg                                                                                                    >  2:142949/1‑130 (MQ=255)
                                                                   ttCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTg                                                                                                    <  1:142949/130‑1 (MQ=255)
                                                                       aGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGGCCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCt                                                                              >  5:95267/1‑148 (MQ=255)
                                                                         gTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGaa                                                                           >  4:309763/1‑149 (MQ=255)
                                                                          ttGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAATCCGGCactgccag                                                                          <  7:506/149‑8 (MQ=255)
                                                                                  cAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGc                                                                  <  5:251894/149‑1 (MQ=255)
                                                                                   aGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                 <  2:107252/149‑1 (MQ=255)
                                                                                            tCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGc                                                         >  5:84174/1‑148 (MQ=255)
                                                                                                aCTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTcgac                                                                                                                                                     <  3:294447/52‑1 (MQ=255)
                                                                                                aCTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTcgac                                                                                                                                                     >  4:294447/1‑52 (MQ=255)
                                                                                                    cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                 <  7:292904/132‑1 (MQ=255)
                                                                                                    cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                 >  8:292904/1‑132 (MQ=255)
                                                                                                    cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGAtt                                                 >  8:264670/1‑148 (MQ=255)
                                                                                                     agcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGTCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTc                                               >  4:254617/1‑149 (MQ=255)
                                                                                                       caCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                             >  6:11340/1‑149 (MQ=255)
                                                                                                       caCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                             <  8:61437/149‑1 (MQ=255)
                                                                                                          gTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCGATTGATGCCAGGGAtttggaggg                                          >  2:174412/1‑143 (MQ=255)
                                                                                                               cATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGATCGGTGTTGCCATTGATGCCAGCGATTTCCACGGTctct                                     <  6:157142/149‑1 (MQ=255)
                                                                                                                aTTAACTCCAGCGGCTTCAGCAGCAGCCAGGTCTACGATGACATGGTCGCCTTTAGCGCCATCGACACTACCTTGAACAATCCTGGCACTGCCTGATTCCCGCAGCTGAACGCTTTTGCAATAGATGACAGCTATTTCCACTCTCTCTa                                    >  6:416545/1‑149 (MQ=255)
                                                                                                                aTTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGgt                                                                       >  8:108580/1‑114 (MQ=255)
                                                                                                                aTTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGgt                                                                       <  7:108580/114‑1 (MQ=255)
                                                                                                                 ttAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                             >  3:112588/1‑139 (MQ=255)
                                                                                                                 ttAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                             <  4:112588/139‑1 (MQ=255)
                                                                                                                   aaCGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACg                                  <  4:44365/148‑1 (MQ=255)
                                                                                                                      gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGTACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTAc                                   <  7:118251/144‑1 (MQ=255)
                                                                                                                      gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGTACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTAc                                   >  8:118251/1‑144 (MQ=255)
                                                                                                                      gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTAt                              >  6:134069/1‑149 (MQ=255)
                                                                                                                         aGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATc                             >  2:127150/1‑147 (MQ=255)
                                                                                                                          gCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAg                          >  2:418675/1‑149 (MQ=255)
                                                                                                                               ttCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGtt                       >  3:394748/1‑147 (MQ=255)
                                                                                                                                tcatcaGCAGACAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACATTAACTTGAACAATGCTTGCAGTGACGGATTCCGGCACCTGCACTGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGATcag                    >  6:31473/1‑149 (MQ=255)
                                                                                                                                 cagcagcagCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTcag                    <  5:416545/148‑1 (MQ=255)
                                                                                                                                    cagcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAgg                >  6:400892/1‑149 (MQ=255)
                                                                                                                                    cagcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAgg                <  1:104692/149‑1 (MQ=255)
                                                                                                                                      gcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCt              <  7:84424/149‑1 (MQ=255)
                                                                                                                                        gaccAGGTCATCGTCGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGt                                         >  6:135096/3‑120 (MQ=255)
                                                                                                                                        gaccAGGTCATCGTCGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGt                                         <  5:135096/118‑1 (MQ=255)
                                                                                                                                         gcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGtt           <  6:84174/149‑1 (MQ=255)
                                                                                                                                          ccAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGtt           >  5:376432/1‑148 (MQ=255)
                                                                                                                                          ccAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTAACTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGTTGTTGCCATTGATGCCAGCGATTTCCACTTTCTCTACGTTATCCAGGTTCAGCAGGCTTTTg          <  5:31473/149‑1 (MQ=255)
                                                                                                                                            aGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGa         <  3:254617/148‑1 (MQ=255)
                                                                                                                                             ggTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATa       <  5:418808/149‑1 (MQ=255)
                                                                                                                                                 gacgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGTCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCt   <  5:11340/149‑1 (MQ=255)
                                                                                                                                                 gacgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCt   <  7:264670/149‑1 (MQ=255)
                                                                                                                                                  acgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTc  >  7:167996/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGAGGTGTCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTC  >  NC_002947/2015109‑2015403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑