Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,015,258 A→T 5.3% I77N (ATC→AAC)  PP_1794 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,015,2580AT 5.3% 236.9 / 2.1 75I77N (ATC→AAC) PP_1794hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/36);  new base T (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GTGTCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTCGA  >  NC_002947/2015114‑2015405
                                                                                                                                                |                                                                                                                                                   
gtgtCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGAc                                                                                                                                                 <  3:63123/149‑1 (MQ=255)
      aCTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTc                                                                                                                                           <  1:105930/149‑1 (MQ=255)
      aCTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCATCGTCGACCTggct                                                                                                                                           >  5:377984/1‑147 (MQ=255)
          cAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGc                                                                                                                                         <  1:415587/147‑1 (MQ=255)
          cAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCGGCAGCAGCAGCCAGGTCGACGATGACCTGGTCGc                                                                                                                                         <  7:90921/147‑1 (MQ=255)
           aGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCATCGTCGACCTggctgctgc                                                                                                                                      <  6:377984/149‑8 (MQ=255)
             ccAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCtt                                                                                                                                      >  8:316765/1‑147 (MQ=255)
             ccAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCtt                                                                                                                                      >  8:104455/1‑147 (MQ=255)
               aaGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAgc                                                                                                                                  >  6:251894/1‑149 (MQ=255)
                   cAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCa                                                                                                                              >  1:107252/1‑149 (MQ=255)
                    aGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCAt                                                                                                                             <  7:328189/149‑1 (MQ=255)
                    aGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCAt                                                                                                                             <  3:336458/149‑1 (MQ=255)
                       gAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCGGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGa                                                                                                                          <  1:291430/149‑1 (MQ=255)
                           aCCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAg                                                                                                                       >  5:51859/1‑148 (MQ=255)
                           aCCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGt                                                                                                                      <  8:76439/149‑1 (MQ=255)
                              tGGGCGTCAGCAGGTGCACCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCAGGTCGCCATTAGCGCCATCGACAGTAc                                                                                                                    >  4:264833/1‑148 (MQ=255)
                                 gCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCtt                                                                                                                 >  8:84424/1‑148 (MQ=255)
                                    tCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAAc                                                                                                             <  3:334197/149‑1 (MQ=255)
                                     cagcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAAc                                                                                                             >  7:61437/1‑148 (MQ=255)
                                       gcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                          >  7:164458/1‑149 (MQ=255)
                                       gcagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                          >  6:418808/1‑149 (MQ=255)
                                        cagGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAAt                                                                                                          >  5:157142/1‑148 (MQ=255)
                                          ggTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCt                                                                                                       <  2:155673/149‑1 (MQ=255)
                                            tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCATCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                     <  5:176130/149‑1 (MQ=255)
                                            tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                     <  7:104455/149‑1 (MQ=255)
                                            tGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTgg                                                                                                     <  8:29238/149‑1 (MQ=255)
                                                gCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGt                                                                                                 <  4:162984/149‑1 (MQ=255)
                                                   ttGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGcc                                                                                              >  3:44365/1‑149 (MQ=255)
                                                       aGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAt                                                                                          <  7:20850/149‑1 (MQ=255)
                                                        gTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAtt                                                                                         <  3:115070/149‑1 (MQ=255)
                                                         ttACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTc                                                                                        >  5:257115/1‑149 (MQ=255)
                                                           aCCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCg                                                                                      >  5:308761/1‑149 (MQ=255)
                                                            ccTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCgg                                                                                     <  6:51859/149‑1 (MQ=255)
                                                            ccTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCg                                                                                      <  7:316765/148‑1 (MQ=255)
                                                             cTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCgg                                                                                     >  1:22960/1‑148 (MQ=255)
                                                              ttCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTg                                                                                                      <  1:142949/130‑1 (MQ=255)
                                                              ttCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTg                                                                                                      >  2:142949/1‑130 (MQ=255)
                                                                  aGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGGCCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCt                                                                                >  5:95267/1‑148 (MQ=255)
                                                                    gTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGaa                                                                             >  4:309763/1‑149 (MQ=255)
                                                                     ttGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGAATCCGGCactgccag                                                                            <  7:506/149‑8 (MQ=255)
                                                                             cAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGc                                                                    <  5:251894/149‑1 (MQ=255)
                                                                              aGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                   <  2:107252/149‑1 (MQ=255)
                                                                                       tCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGc                                                           >  5:84174/1‑148 (MQ=255)
                                                                                               cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                   <  7:292904/132‑1 (MQ=255)
                                                                                               cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGcc                                                                   >  8:292904/1‑132 (MQ=255)
                                                                                               cagcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGAtt                                                   >  8:264670/1‑148 (MQ=255)
                                                                                                agcaCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGTCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTc                                                 >  4:254617/1‑149 (MQ=255)
                                                                                                  caCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                               <  8:61437/149‑1 (MQ=255)
                                                                                                  caCGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                               >  6:11340/1‑149 (MQ=255)
                                                                                                     gTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCGATTGATGCCAGGGAtttggaggg                                            >  2:174412/1‑143 (MQ=255)
                                                                                                          cATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGATCGGTGTTGCCATTGATGCCAGCGATTTCCACGGTctct                                       <  6:157142/149‑1 (MQ=255)
                                                                                                           aTTAACTCCAGCGGCTTCAGCAGCAGCCAGGTCTACGATGACATGGTCGCCTTTAGCGCCATCGACACTACCTTGAACAATCCTGGCACTGCCTGATTCCCGCAGCTGAACGCTTTTGCAATAGATGACAGCTATTTCCACTCTCTCTa                                      >  6:416545/1‑149 (MQ=255)
                                                                                                           aTTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGgt                                                                         <  7:108580/114‑1 (MQ=255)
                                                                                                           aTTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGgt                                                                         >  8:108580/1‑114 (MQ=255)
                                                                                                            ttAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                               <  4:112588/139‑1 (MQ=255)
                                                                                                            ttAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCa                                               >  3:112588/1‑139 (MQ=255)
                                                                                                              aaCGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACg                                    <  4:44365/148‑1 (MQ=255)
                                                                                                                 gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGTACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTAc                                     >  8:118251/1‑144 (MQ=255)
                                                                                                                 gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGTACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTAc                                     <  7:118251/144‑1 (MQ=255)
                                                                                                                 gCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTAt                                >  6:134069/1‑149 (MQ=255)
                                                                                                                    aGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATc                               >  2:127150/1‑147 (MQ=255)
                                                                                                                     gCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAg                            >  2:418675/1‑149 (MQ=255)
                                                                                                                          ttCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGtt                         >  3:394748/1‑147 (MQ=255)
                                                                                                                           tcatcaGCAGACAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACATTAACTTGAACAATGCTTGCAGTGACGGATTCCGGCACCTGCACTGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGATcag                      >  6:31473/1‑149 (MQ=255)
                                                                                                                            cagcagcagCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTcag                      <  5:416545/148‑1 (MQ=255)
                                                                                                                               cagcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAgg                  <  1:104692/149‑1 (MQ=255)
                                                                                                                               cagcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAgg                  >  6:400892/1‑149 (MQ=255)
                                                                                                                                 gcagcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCt                <  7:84424/149‑1 (MQ=255)
                                                                                                                                   gaccAGGTCATCGTCGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGt                                           >  6:135096/3‑120 (MQ=255)
                                                                                                                                   gaccAGGTCATCGTCGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGt                                           <  5:135096/118‑1 (MQ=255)
                                                                                                                                    gcCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGtt             <  6:84174/149‑1 (MQ=255)
                                                                                                                                     ccAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGtt             >  5:376432/1‑148 (MQ=255)
                                                                                                                                     ccAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTAACTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGTTGTTGCCATTGATGCCAGCGATTTCCACTTTCTCTACGTTATCCAGGTTCAGCAGGCTTTTg            <  5:31473/149‑1 (MQ=255)
                                                                                                                                       aGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGa           <  3:254617/148‑1 (MQ=255)
                                                                                                                                        ggTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATa         <  5:418808/149‑1 (MQ=255)
                                                                                                                                            gacgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGTCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCt     <  5:11340/149‑1 (MQ=255)
                                                                                                                                            gacgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCt     <  7:264670/149‑1 (MQ=255)
                                                                                                                                             acgaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTc    >  7:167996/1‑149 (MQ=255)
                                                                                                                                               gaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTCGa  >  2:210487/1‑149 (MQ=255)
                                                                                                                                               gaTGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTCGa  <  6:257115/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTGTCGACTGCAGCCAAGGCAGCGAAGACCTGGGCGTCAGCAGGTGCAGCGTTGGAGTTACCTTCCAGGTTGACTACCAGGTTGGCATCCGACTGCAGCACGTAGGCATTAACGCCAGCGGCTTCAGCAGCAGCCAGGTCGACGATGACCTGGTCGCCTTTAGCGCCATCGACAGTACCTTGAACAATGCTGGCAGTGCCGGATTCCGGCAGCTGAACGGTGTTGCCATTGATGCCAGCGATTTCCACGGTCTCTACGTTATCCAGGTTCAGCAGGCTGTTGATAGCCTCGA  >  NC_002947/2015114‑2015405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑