Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,025,714	G→T	5.5%	Q238H (CAG→CAT) ‡	wbpZ →	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,025,714	0	G	T	5.5%	295.1 / 5.3	90	Q238H (CAG→CAT) ‡	wbpZ	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (42/43);  new base T (2/3);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCGG  >  NC_002947/2025571‑2025859                                                                                                                                                 |                                                                                                                                                   cgcaTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc                                                                                                                                               <  3:190337/149‑1 (MQ=255)  gcaTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc‑t                                                                                                                                             >  2:272239/1‑149 (MQ=255)  gcaTGAGCGAGCGCTTTTCCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc‑t                                                                                                                                             <  8:289946/149‑1 (MQ=255)   caTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc‑t                                                                                                                                             >  6:394153/1‑148 (MQ=255)    aTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc‑t                                                                                                                                             >  4:127594/1‑147 (MQ=255)    aTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGgctgc‑t                                                                                                                                             <  3:127594/147‑1 (MQ=255)    aTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTC‑AGCAGCCTgctgc‑tt                                                                                                                                            >  8:307294/1‑146 (MQ=255)     tGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTC‑AGCAGCCTgctgc‑ttgag                                                                                                                                         <  4:352162/149‑5 (MQ=255)       agcgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCCTgctgc‑ttgagc                                                                                                                                        <  6:196155/149‑6 (MQ=255)        gcgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCt                                                                                                                                        >  6:101160/1‑148 (MQ=255)        gcgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTc                                                                                                                                       <  3:54526/149‑1 (MQ=255)         cgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCt                                                                                                                                        >  6:247789/1‑147 (MQ=255)         cgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtgc                                                                                                                                      <  7:307294/149‑3 (MQ=255)          gagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAg                                                                                                                                     <  2:146954/149‑1 (MQ=255)            gcgcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAATGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAgg                                                                                                                                    <  1:272239/149‑1 (MQ=255)            gcgcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGc                                                                                                                                   <  3:292399/149‑1 (MQ=255)              gcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAAGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCtt                                                                                                                                 <  6:50143/149‑1 (MQ=255)               cTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTg                                                                                                                                <  4:102068/149‑1 (MQ=255)                   ttCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACa                                                                                                                             <  1:297834/148‑1 (MQ=255)                     ccTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAAt                                                                                                                           <  5:67527/148‑1 (MQ=255)                      cTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGt                                                                                                                         >  6:423275/1‑149 (MQ=255)                       tGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGTGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGGA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGAc                                                                                                                              >  6:119008/1‑143 (MQ=255)                       tGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGTGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGGA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGAc                                                                                                                              <  5:119008/143‑1 (MQ=255)                         tttGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCa                                                                                                                      <  8:75127/149‑1 (MQ=255)                          ttGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAg                                                                                                                     >  3:94678/1‑149 (MQ=255)                             tAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTc                                                                                                                  <  5:101160/149‑1 (MQ=255)                             tAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTc                                                                                                                  >  6:214425/1‑149 (MQ=255)                             tAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTc                                                                                                                  >  1:370988/1‑149 (MQ=255)                               ggCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCt                                                                                                                <  7:334251/149‑1 (MQ=255)                               ggCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGCTcc                                                                                                                 <  8:64109/148‑1 (MQ=255)                               ggCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGATGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCTGTTCCt                                                                                                                <  1:122872/149‑1 (MQ=255)                                        cGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCt                                                                                                       <  1:62740/149‑1 (MQ=255)                                         ggTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTg                                                                                                      <  7:11478/149‑1 (MQ=255)                                         ggTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTg                                                                                                      >  8:55274/1‑149 (MQ=255)                                           tactacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTgg                                                                                                     >  3:65142/1‑148 (MQ=255)                                           tactacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGc                                                                                                    <  4:65142/149‑1 (MQ=255)                                               acAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGAc                                                                                                >  5:412391/1‑149 (MQ=255)                                                     ggCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCCGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGa                                                                                           >  1:338278/1‑148 (MQ=255)                                                      gCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACa                                                                                         <  1:311762/149‑1 (MQ=255)                                                                 tGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGTCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCATGGGCAGCCTGGCAGACGATGACATAGCGGCgctg                                                                              >  6:36983/1‑149 (MQ=255)                                                                   cTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCgctgc                                                                             <  5:214425/148‑1 (MQ=255)                                                                     aCAGGCTGCGTCTATCAACCGTCTGCCGGTGGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGctgctg                                                                           >  4:110352/1‑148 (MQ=255)                                                                      cAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTgg                                                                          <  2:370988/148‑1 (MQ=255)                                                                      cAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTgg                                                                          <  7:281511/148‑1 (MQ=255)                                                                       aGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAg                                                                        >  4:128789/1‑149 (MQ=255)                                                                       aGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAg                                                                        >  2:369101/1‑149 (MQ=255)                                                                         gCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCTTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCt                                                                      <  2:23939/149‑1 (MQ=255)                                                                         gCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCt                                                                      >  1:297086/1‑149 (MQ=255)                                                                             cGTCTATCAACCGTCTGCCGGTGGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCtgt                                                                    <  3:110352/147‑1 (MQ=255)                                                                              gTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGtt                                                                 >  8:368051/1‑149 (MQ=255)                                                                                cTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTAt                                                               >  3:205098/1‑149 (MQ=255)                                                                                 tctCAACAGTCTGCAGGTAGTGATGATCTGCTGTGGCTTCCTCGAA‑GCGCAGGTCAAGCAGCAGGCTGC‑TGAGCTCAGTCTTGACAATGTTCAGTTCCTGGGCAGCCTGTGAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTAt                                                               <  7:397888/146‑1 (MQ=255)                                                                                 tATCACCCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAAAGTTCAGTTCCAGGGCAGCCTGGCAGACGCTGACAGCGCGGCGCGGCTGGAGCTGTGTTATg                                                              >  4:93253/1‑149 (MQ=255)                                                                                   tCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCa                                                            >  2:281217/1‑149 (MQ=255)                                                                                     aaacGTCTGCCGGTAGTGAACATCGGCGGTGGCTACCTCTAA‑GCCCAGCTCAAGCAGCAGGATTC‑TGATCTCAGGCTTGACAATTTTCAGTTCCTGGGCAGCCTCGCAGACGATGACAGAGCGGCGCTGCTGGAGCAGAGTTATgaga                                                           >  8:397888/4‑145 (MQ=255)                                                                                     aaCCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGTAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCAtt                                                          <  2:135384/149‑1 (MQ=255)                                                                                     aaCCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCAtt                                                          >  5:95816/1‑149 (MQ=255)                                                                                     aaCCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCAtt                                                          >  1:375998/1‑149 (MQ=255)                                                                                      aCCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTc                                                         <  1:408063/149‑1 (MQ=255)                                                                                        cGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCg                                                        >  6:210964/1‑148 (MQ=255)                                                                                                ggTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTccat                                               <  5:423275/149‑1 (MQ=255)                                                                                                 gTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTccatc                                              <  7:368051/149‑1 (MQ=255)                                                                                                       atcatcGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTT‑CATCCCATCTg                                       >  7:338896/1‑149 (MQ=255)                                                                                                        tcatcGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTg                                       <  1:281217/149‑1 (MQ=255)                                                                                                         catcGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGc                                      >  8:230284/1‑149 (MQ=255)                                                                                                           tcGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGt                                    <  4:94678/149‑1 (MQ=255)                                                                                                              gcggTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTcc                                 >  7:22987/1‑149 (MQ=255)                                                                                                              gcggTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTcc                                 <  2:297086/149‑1 (MQ=255)                                                                                                                ggTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGATTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGa                               <  1:257227/149‑1 (MQ=255)                                                                                                                 gTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAg                              <  2:375998/149‑1 (MQ=255)                                                                                                                      ttCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTc                                                         >  6:284736/1‑117 (MQ=255)                                                                                                                      ttCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTc                                                         <  5:284736/117‑1 (MQ=255)                                                                                                                      ttCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGtt                         >  1:170454/1‑149 (MQ=255)                                                                                                                      ttCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGtt                         >  8:268085/1‑149 (MQ=255)                                                                                                                         cTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg                      >  1:30240/1‑149 (MQ=255)                                                                                                                          tCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg                      >  1:318388/1‑148 (MQ=255)                                                                                                                          tCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg                      >  2:268236/1‑148 (MQ=255)                                                                                                                          tCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg                      >  7:38744/1‑148 (MQ=255)                                                                                                                          tCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGGGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg                      >  6:115105/1‑148 (MQ=255)                                                                                                                           cGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCa                    >  5:146757/1‑149 (MQ=255)                                                                                                                            gAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGc                     <  7:55274/147‑1 (MQ=255)                                                                                                                              a‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTTGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAttt                 <  6:146757/149‑1 (MQ=255)                                                                                                                              a‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAttt                 <  6:412391/149‑1 (MQ=255)                                                                                                                               gcctgaGCTC‑AGCAGCCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCAttt                 >  6:135615/6‑149 (MQ=255)                                                                                                                                 cgcAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTtct               >  1:275063/1‑149 (MQ=255)                                                                                                                                    aGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctg            <  3:128789/149‑1 (MQ=255)                                                                                                                                    aGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctg            <  2:170454/149‑1 (MQ=255)                                                                                                                                     gCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgc           >  3:3498/1‑149 (MQ=255)                                                                                                                                     gCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgc           >  8:411851/1‑149 (MQ=255)                                                                                                                                         aaGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgctg         <  2:30240/147‑1 (MQ=255)                                                                                                                                            cagcagGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGc    <  1:419837/149‑1 (MQ=255)                                                                                                                                             agcagGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCg   <  7:268085/149‑1 (MQ=255)                                                                                                                                               cagGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCgg  >  6:64585/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CGCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGTGGCTTCCTCGAA‑GCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCGG  >  NC_002947/2025571‑2025859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑