Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,038,564 G→A 5.8% L306L (CTG→TTG)  rffE ← UDP‑N‑acetylglucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,038,5640GA 5.8% 209.3 / 2.1 70L306L (CTG→TTG) rffEUDP‑N‑acetylglucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (34/31);  new base A (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATCCCCCAACAG  >  NC_002947/2038419‑2038705
                                                                                                                                                 |                                                                                                                                             
tGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCAc                                                                                                                                            >  3:28653/1‑149 (MQ=255)
 gggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAACAcc                                                                                                                                           >  5:193079/1‑149 (MQ=255)
   gCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCgg                                                                                                                                         >  5:185038/1‑149 (MQ=255)
    cGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGt                                                                                                                                        <  6:325922/149‑1 (MQ=255)
    cGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGt                                                                                                                                        <  7:147008/149‑1 (MQ=255)
        gCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGc                                                                                                                                    >  2:406764/1‑149 (MQ=255)
            aTTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGccc                                                                                                                                  <  4:282458/147‑1 (MQ=255)
             tttCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAgg                                                                                                                               <  6:386739/149‑1 (MQ=255)
                cgcgATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGcc                                                                                                                            >  4:319905/1‑149 (MQ=255)
                 gcgATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCg                                                                                                                           >  1:290940/1‑149 (MQ=255)
                  cgATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCgg                                                                                                                          <  7:31317/149‑1 (MQ=255)
                   gATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGgc                                                                                                                         >  1:188211/1‑149 (MQ=255)
                   gATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGgc                                                                                                                         <  3:359087/149‑1 (MQ=255)
                   gATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTCCCCCCACGGCTTCTGGTCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCgcc                                                                                                                         >  3:76921/1‑147 (MQ=255)
                       cgccgcCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAACACCGGTTTGCCCAGGGCCGGCGCtt                                                                                                                     <  6:193079/149‑1 (MQ=255)
                               cATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCgg                                                                                                                          <  3:403627/136‑1 (MQ=255)
                               cATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCgg                                                                                                                          >  4:403627/1‑136 (MQ=255)
                                 tCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAAt                                                                                                           <  1:406764/149‑1 (MQ=255)
                                    gTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGc                                                                                                         >  2:230186/1‑148 (MQ=255)
                                     tCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCa                                                                                                       >  7:176407/1‑149 (MQ=255)
                                     tCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAACACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCa                                                                                                       >  6:194793/1‑149 (MQ=255)
                                            ggTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGt                                                                                                >  6:391403/1‑149 (MQ=255)
                                                cTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCgg                                                                                             <  2:188211/148‑1 (MQ=255)
                                                cTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCgg                                                                                             <  3:319905/148‑1 (MQ=255)
                                                   aaCGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGt                                                                                            >  3:148202/1‑146 (MQ=255)
                                                   aaCGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGt                                                                                            >  3:425060/1‑146 (MQ=255)
                                                    aCGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGa                                                                                        <  3:125625/149‑1 (MQ=255)
                                                    aCGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGa                                                                                        <  4:148202/149‑1 (MQ=255)
                                                      gCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAAt                                                                                      >  1:207957/1‑149 (MQ=255)
                                                        ccGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATc                                                                                     >  3:160913/1‑148 (MQ=255)
                                                                   aTGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCgg                                                                         >  7:262666/1‑149 (MQ=255)
                                                                   aTGCTACTTCGGGGCCCCCCAGCTGGACCGTACCTTCCGCCCCGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTAGGTCAGAATCACATAGTCCCgg                                                                         <  7:306582/149‑1 (MQ=255)
                                                                    tGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCggg                                                                        >  4:137851/1‑149 (MQ=255)
                                                                    tGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCggg                                                                        >  5:237585/1‑149 (MQ=255)
                                                                         gttCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATg                                                                   <  4:76921/148‑1 (MQ=255)
                                                                         cTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCAt                                                                    >  1:222512/1‑148 (MQ=255)
                                                                         cTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATg                                                                   <  2:290940/149‑1 (MQ=255)
                                                                             gttGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAgg                                                                <  7:401052/146‑1 (MQ=255)
                                                                             ggTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAgg                                                                <  1:230186/148‑1 (MQ=255)
                                                                               tGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTaa                                                             <  8:262666/149‑1 (MQ=255)
                                                                                 cccACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTaaa                                                            <  4:28653/148‑1 (MQ=255)
                                                                                   caccaGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTcctcct                                                                                                               >  2:351817/1‑95 (MQ=255)
                                                                                   caccaGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAaacg                                                          >  4:404677/1‑148 (MQ=255)
                                                                                    accaGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGcc                                                                            >  1:198658/1‑129 (MQ=255)
                                                                                    accaGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGcc                                                                            <  2:198658/129‑1 (MQ=255)
                                                                                       aGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACgg                                                     <  4:384419/149‑1 (MQ=255)
                                                                                                gTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATc                                            <  6:237585/149‑1 (MQ=255)
                                                                                                 tACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATc                                            >  6:376799/1‑148 (MQ=255)
                                                                                                 tACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCAGGAGGTAAACGAACGGCAGGTAATc                                            >  7:401362/1‑148 (MQ=255)
                                                                                                   ccTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAg                                         <  3:137851/149‑1 (MQ=255)
                                                                                                       ccgccACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCg                                       <  8:176407/147‑1 (MQ=255)
                                                                                                            aCGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGa                                 >  8:117364/1‑148 (MQ=255)
                                                                                                                      ggCGCTCGGTAGTGTCGCGCATCACCATCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTgg                         <  3:93590/146‑1 (MQ=255)
                                                                                                                      ggCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATg                      >  5:299388/1‑149 (MQ=255)
                                                                                                                      ggCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATg                      <  6:185038/149‑1 (MQ=255)
                                                                                                                       gcgcTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGAc                                <  1:214469/138‑1 (MQ=255)
                                                                                                                       gcgcTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGAc                                >  2:214469/1‑138 (MQ=255)
                                                                                                                             ggTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTca               >  4:141142/1‑149 (MQ=255)
                                                                                                                             ggTAGTGTCGCGCATCACCAACACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTc                <  5:194793/148‑1 (MQ=255)
                                                                                                                                 gtgtCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATc           >  1:70319/1‑149 (MQ=255)
                                                                                                                                 gtgtCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATc           >  1:76545/1‑149 (MQ=255)
                                                                                                                                    gcgcgCATCACCAGCACCGGATTGCCCACGGCCGGCGCTTCCTCCTGAATGCCACCCGAGGCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTGCGACAAGGTGGATGTTGCTCACATcccc        <  4:88621/148‑1 (MQ=255)
                                                                                                                                      gcgcATCACCAGCACCGGTTTTCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCGGAGTCGGTCATAATCACATAGGCCCTGGTCATGTGGTAAACTAAGTTCAGGTAATTCAGCGGTTCGACAATGTTGATGATGCTCACATccccc       <  6:331052/148‑1 (MQ=255)
                                                                                                                                      gcgcATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATccccc       <  2:222512/148‑1 (MQ=255)
                                                                                                                                      gcgcATCACCAGCACCGGTTTGCACAGGGACGGCGCTTCATCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGACCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGATGCTCACATccccc       >  5:331052/1‑148 (MQ=255)
                                                                                                                                       cgcATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATCCCCCaa     <  3:141142/149‑1 (MQ=255)
                                                                                                                                        gcATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTACCTCCTGAATGCCACCCGAGTCGGTCAGAAACACCAAGGCCCGGGACATGAGGTAAGCGAACGCCACGGCATCCCGCGGTTCGACAAGGTGGATGTTGCTCACATCCCCCAAc    >  6:368672/1‑149 (MQ=255)
                                                                                                                                          aTCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATCCCCCAACAg  >  7:294721/1‑149 (MQ=255)
                                                                                                                                            cACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACAt            <  1:333090/137‑1 (MQ=255)
                                                                                                                                            cACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACAt            >  2:333090/1‑137 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGGTAAACGAACGGCAGGTAATCCAGCGGTTCGACAAGGTGGATGTTGCTCACATCCCCCAACAG  >  NC_002947/2038419‑2038705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑