Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,053,627	T→C	6.3%	intergenic (‑27/+39)	PP_1828 ← / ← PP_1829	hypothetical protein/alpha/beta family hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,053,627	0	T	C	6.3%	179.4 / 2.0	63	intergenic (‑27/+39)	PP_1828/PP_1829	hypothetical protein/alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base C (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GGTCGGCAGTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGCTCATGCCCTTGG  >  NC_002947/2053481‑2053775                                                                                                                                                    |                                                                                                                                                      ggtcggCAGTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCctgctg                                                                                                                                                      >  4:285750/1‑148 (MQ=255)    cggCTGTGCTGCCGGGCGTAGCTGCTGGAATTGTTGGGCGGTCAGGTGGGC‑CCTGGGCTGGGGGGGGGTTTCCTGGGGTTCTTGGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATc                                                                                                                                                  <  3:399199/149‑1 (MQ=255)     ggCAGTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCg                                                                                                                                                 >  3:343335/1‑149 (MQ=255)       cAGTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCgg                                                                                                                                                >  8:199409/1‑148 (MQ=255)       cAGTGCTGCCAGGCGTAGCAGCTGCAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCggc                                                                                                                                               <  2:878/149‑1 (MQ=255)         gTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTGTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCggcag                                                                                                                                             <  3:44581/149‑1 (MQ=255)            ctgcCAGGCGTAGCAGGTGGAAATGTTCGGCGGTCAGCGCGGC‑CCTGGGCAGGGGGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCggcaggc                                                                                                                                           <  1:162740/148‑1 (MQ=255)               ccAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCCGATACAGCAGGCAt                                                                                                                                         <  2:294987/147‑1 (MQ=255)                 aGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCt                                                                                                                                     >  5:270214/1‑149 (MQ=255)                     gTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTgaga                                                                                                                                 >  6:434276/1‑149 (MQ=255)                     gTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTgaga                                                                                                                                 >  6:20099/1‑149 (MQ=255)                        ccagcTGGAAATGTTCGGCGGTCAGCTCGGC‑CCCGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGCCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCt                                                                                                                              <  7:126236/148‑1 (MQ=255)                             tGGAAATGTTCGGCGGTCAGCTCGGCGCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGc                                                                                                                          >  1:4405/1‑149 (MQ=255)                                aatggtgcggcggGCCGCGCGGC‑CCAGGGCAGGGGGGGGGTTCCCGGGGTTGCTTCGGCCGGCGCGCCGGCGGTGGGGGAGGTGGTCATCGGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGt                                                                                                                      <  6:310090/142‑1 (MQ=255)                                 aaTGTTCGGCGGTCAGCTCGGC‑CCA‑GGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGttt                                                                                                                    >  6:8120/1‑149 (MQ=255)                                   tGTTGGGGGGTCTGGTGGGC‑GCTGGGCAGGGGGGGGGTTTCTTGGGGTTCTTGGGCGGGGTGGAGGGGGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGttt                                                                                                                    <  4:343335/148‑1 (MQ=255)                                    gTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTg                                                                                                                   >  2:218964/1‑148 (MQ=255)                                             tCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCCGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGcc                                                                                                          >  5:167903/1‑148 (MQ=255)                                               aGCTGGGC‑CCAGGGCAGGGCTGGTGTTTCGGGGGGTGCTTCGGCCGGCTCGAGGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGTCTGAGACCTGAGCCTGTTTGACTTACGCCGa                                                                                                        <  6:386594/148‑1 (MQ=255)                                               aGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCg                                                                                                         >  3:271080/1‑147 (MQ=255)                                                ggtCGGG‑CCCGGGCAGGGGGGGGGGTTCCGGGGGTTCTGCTGCCGGCTCGACGGCGGTGGGGGAGGTTGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAg                                                                                                      <  7:401929/147‑1 (MQ=255)                                                gcgcGGC‑CCAGGGCTGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAg                                                                                                      <  6:37757/146‑1 (MQ=255)                                                gCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                            <  2:126794/127‑1 (MQ=255)                                                gCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                            >  1:126794/1‑127 (MQ=255)                                                    ggC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCa                                                                                                   >  3:180106/1‑148 (MQ=255)                                                      c‑ccAGGGCAGGGCGGGGGTTTCGTGGGGGTCTTCGGCCGGCTCGCCGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAAt                                                                                                <  5:107938/149‑1 (MQ=255)                                                      c‑ccAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                            <  6:327273/121‑1 (MQ=255)                                                      c‑ccAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                            >  5:327273/1‑121 (MQ=255)                                                          cgggctgggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCGCGACGGGGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCt                                                                                             <  4:180106/148‑1 (MQ=255)                                                          agggcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCt                                                                                             <  2:19692/149‑1 (MQ=255)                                                            ggcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCCGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTgg                                                                                           <  6:167903/149‑1 (MQ=255)                                                                 ggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGaa                                                                                      >  4:330175/1‑149 (MQ=255)                                                                 ggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGaa                                                                                      >  5:34909/1‑149 (MQ=255)                                                                  gggggggTTTCCTGGGTGTCTTCGGCCGGCTCTACGGCGGGTGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAATGCAAATGCTGGATGAAc                                                                                     <  4:271080/147‑1 (MQ=255)                                                                           tCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAg                                                                                                      <  6:111857/123‑1 (MQ=255)                                                                           tCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAg                                                                                                      >  5:111857/1‑123 (MQ=255)                                                                            ccTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATAAGGGCTTGTCATCAGCTATGCCTCCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGAATTACGCCGATGGAAAATGATGGATGAACTTccaaatac                                                                           >  4:149177/1‑142 (MQ=255)                                                                             cTGGGGTTCTTCGGCCGGCTCGACGGGGGT‑GGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgc                                                                         <  6:40102/149‑1 (MQ=255)                                                                              tGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgc                                                                         >  4:351428/1‑149 (MQ=255)                                                                               ggggTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgct                                                                        >  6:44234/1‑149 (MQ=255)                                                                                gggTTCTTGCGCCGGCGCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgctc                                                                       <  3:149177/149‑1 (MQ=255)                                                                                gggTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTTGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgctc                                                                       <  7:143596/149‑1 (MQ=255)                                                                                gggTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgctc                                                                       <  6:270214/149‑1 (MQ=255)                                                                                     cttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGccc                                                                   <  5:434276/148‑1 (MQ=255)                                                                                     cttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGccc                                                                   <  5:20099/148‑1 (MQ=255)                                                                                                     ggcggTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCgg                                                  <  8:115868/149‑1 (MQ=255)                                                                                                        ggTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGTAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAg                                               >  4:290476/1‑149 (MQ=255)                                                                                                         gTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGc                                              >  6:57819/1‑149 (MQ=255)                                                                                                            ggggAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCg                                           <  6:34909/149‑1 (MQ=255)                                                                                                              ggAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCggg                                         <  3:330175/149‑1 (MQ=255)                                                                                                                aGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACgcgc                                                        <  3:62235/132‑1 (MQ=255)                                                                                                                aGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTAACgcgc                                                        >  4:62235/1‑132 (MQ=255)                                                                                                                    ggtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAg                                   >  1:186026/1‑149 (MQ=255)                                                                                                                          caGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCt                             >  1:267467/1‑149 (MQ=255)                                                                                                                            gggCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGt                           >  4:149137/1‑149 (MQ=255)                                                                                                                                  gTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTcgcg                       <  7:199409/147‑1 (MQ=255)                                                                                                                                  gTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgct                     >  4:37100/1‑149 (MQ=255)                                                                                                                                    ccTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctg‑c                  >  2:231805/1‑149 (MQ=255)                                                                                                                                     cTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctg‑ct                 >  6:215415/1‑149 (MQ=255)                                                                                                                                      tCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGctg‑ctg                >  8:300689/1‑149 (MQ=255)                                                                                                                                        aGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGc              >  1:74630/1‑149 (MQ=255)                                                                                                                                        aGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGc              >  5:407919/1‑149 (MQ=255)                                                                                                                                          cTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAACTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGCt             >  7:246316/1‑148 (MQ=255)                                                                                                                                               ccTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTAAGGTAGCTGTCGCGCTGTCTGGCTCATGc        >  1:368214/1‑149 (MQ=255)                                                                                                                                                    tgTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGCTCATGCCCTTgg  >  5:279129/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGTCGGCAGTGCTGCCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGC‑CCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTG‑CTGGCTCATGCCCTTGG  >  NC_002947/2053481‑2053775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑