Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,098,760 A→T 5.0% I377N (ATT→AAT)  PP_1875 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,098,7600AT 5.0% 248.3 / 2.2 79I377N (ATT→AAT) PP_1875two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (37/38);  new base T (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

TGACGATCTGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAGGGCGA  >  NC_002947/2098614‑2098907
                                                                                                                                                   |                                                                                                                                                     
tGACGATCTGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCATTg                                                                                                                                                     >  3:340599/1‑149 (MQ=255)
   cGATCTGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGt                                                                                                                                                  <  5:240496/149‑1 (MQ=255)
    gATCTGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGtt                                                                                                                                                 >  8:23300/1‑149 (MQ=255)
     atatGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTc                                                                                                                                                >  3:73875/4‑149 (MQ=255)
         gCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGc                                                                                                                                            >  2:421351/1‑149 (MQ=255)
          ccGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCATTGCGTTcgac                                                                                                                                             <  4:340599/147‑4 (MQ=255)
          ccGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCATTGCGTTcga                                                                                                                                              <  1:134585/146‑3 (MQ=255)
            gCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGct‑‑ct                                                                                                                                       <  2:102195/149‑1 (MQ=255)
            gCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGct‑‑c                                                                                                                                        >  4:420172/1‑148 (MQ=255)
                    gTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGcc                                                                                                                               >  7:307158/1‑149 (MQ=255)
                         gTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACTCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCAt                                                                                                                          <  4:73875/149‑1 (MQ=255)
                            gCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCg                                                                                                                        >  1:398423/1‑148 (MQ=255)
                             ccGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGaa                                                                                                                      <  5:212162/149‑1 (MQ=255)
                                 gCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTg                                                                                                                  <  5:247549/149‑1 (MQ=255)
                                 gCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTg                                                                                                                  <  3:429540/149‑1 (MQ=255)
                                  cACGCTGCTGGGGAAGTCACTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTg                                                                                                                  >  1:123577/1‑148 (MQ=255)
                                       tgctgGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCGACGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACaa                                                                                                             >  1:102984/1‑149 (MQ=255)
                                           ggggAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTc                                                                                                          >  3:338192/1‑147 (MQ=255)
                                              gAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGt                                                                                                           <  4:420119/143‑1 (MQ=255)
                                              gAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGt                                                                                                           >  3:420119/1‑143 (MQ=255)
                                              gAAGGCGCTGGCGATCAGGCAGGTCAGCTACACTCTTTGCG‑CTTTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGaa                                                                                                     <  4:271770/149‑1 (MQ=255)
                                                    gCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCg                                                                                                <  3:164231/148‑1 (MQ=255)
                                                     cTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGtt                                                                                              <  2:123577/149‑1 (MQ=255)
                                                        gCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTc                                                                                             <  6:191547/147‑1 (MQ=255)
                                                         cGATCAGGCAGGGCAGCGCCACACTGTGCG‑CGGTGTTCTGCGTCAGCAGGTTGGCGGTGTCTTCCCCCATCGAGGCCCTGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGGCAAGAATATCGTTcagc                                                                                          <  2:405342/149‑1 (MQ=255)
                                                          gATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTcag                                                                                           >  2:205652/1‑147 (MQ=255)
                                                          gATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTcag                                                                                           >  6:325706/1‑147 (MQ=255)
                                                           aTCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTcagcag                                                                                        <  2:398423/149‑1 (MQ=255)
                                                            tCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCagcagc                                                                                       >  8:46441/1‑149 (MQ=255)
                                                                 caggTCAGCTCCACACTTTGCG‑CTGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTAc                                                                                  <  6:116117/149‑1 (MQ=255)
                                                                  aggTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTAcc                                                                                 <  1:205652/149‑1 (MQ=255)
                                                                     tCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGc                                                                              <  6:388214/149‑1 (MQ=255)
                                                                       aGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGTTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCAc                                                                            >  2:140749/1‑149 (MQ=255)
                                                                         cTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCt                                                                          >  7:73433/1‑149 (MQ=255)
                                                                          tCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCAATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCtt                                                                         >  2:276456/1‑149 (MQ=255)
                                                                            cacaCTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCtt                                                                         <  8:307158/147‑1 (MQ=255)
                                                                             acacTTTGCG‑CTGTGTTCTGCGACAGCAGGTTGGCTGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTTGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGcc                                                                      <  7:118493/149‑1 (MQ=255)
                                                                              cacTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGccc                                                                     <  8:369970/149‑1 (MQ=255)
                                                                              cacTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGccc                                                                     <  8:300557/149‑1 (MQ=255)
                                                                                 tttGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAg                                                                  >  8:345572/1‑149 (MQ=255)
                                                                                 attGCG‑CGCTGTTCTGCGACACCAGGTTGGCGGTCTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCACCA‑‑CAAGCTGCCCGGCATCGAACTTGGACAACTCAAGAATAACCTTCACCAGCTCAACCAGCACCTAGCCCGAg                                                                  >  6:136706/2‑149 (MQ=255)
                                                                                     cg‑cgGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGTTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCAt                                                              <  1:140749/149‑1 (MQ=255)
                                                                                        cgGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATgg                                                            <  7:291644/149‑1 (MQ=255)
                                                                                        cgGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATgg                                                            >  5:221033/1‑149 (MQ=255)
                                                                                        cgGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATgg                                                            <  1:421351/149‑1 (MQ=255)
                                                                                        cgGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATgg                                                            >  2:47392/1‑149 (MQ=255)
                                                                                         ggTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATggg                                                           <  3:219839/149‑1 (MQ=255)
                                                                                         ggTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATggg                                                           <  1:47392/149‑1 (MQ=255)
                                                                                          gtgtTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGc                                                          >  8:402118/1‑149 (MQ=255)
                                                                                               cTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATa                                                      <  2:296053/148‑1 (MQ=255)
                                                                                               cTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAg                                                     >  2:182659/1‑149 (MQ=255)
                                                                                                tGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCTGGATCGCTGGCCGGCAGGGAAATGGGAAAATTAAAGAATAAGTTACAGCAGATATACCAGCCCATTGCCCGGGTCATGGGCGATa                                                      >  1:348167/1‑149 (MQ=255)
                                                                                                tGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATa                                                      <  5:98955/147‑1 (MQ=255)
                                                                                                tGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATa                                                      >  6:98955/1‑147 (MQ=255)
                                                                                                tGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGc                                                    >  8:218683/1‑149 (MQ=255)
                                                                                                  cGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAAGTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGc                                                    >  8:122608/1‑147 (MQ=255)
                                                                                                   gACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAg                                                 >  1:273362/1‑149 (MQ=255)
                                                                                                              ggCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGt                                       <  7:23300/148‑1 (MQ=255)
                                                                                                                cGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATTCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGc                                    <  1:276456/149‑1 (MQ=255)
                                                                                                                       ttCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATa                                                      <  2:429128/124‑1 (MQ=255)
                                                                                                                       ttCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATa                                                      >  1:429128/1‑124 (MQ=255)
                                                                                                                       ttCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTgg                             >  4:191373/1‑149 (MQ=255)
                                                                                                                         ccaccaTCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTGAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCCTTGCTCGCTggg                            >  6:291232/1‑148 (MQ=255)
                                                                                                                         ccaccaTCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGTGTTGCTCGCTgg                             <  5:325706/147‑1 (MQ=255)
                                                                                                                         ccaccaTCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTg                              <  7:244286/146‑1 (MQ=255)
                                                                                                                         ccaccaTCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAACAATATAGTTCAGCAGATCTACCAGCACATTGCCCGAGTCATGGGCGATAGCAAGTTGCTGGCGTTGCTCGCTggg                            >  1:61191/1‑148 (MQ=255)
                                                                                                                         ccaccaTCGAGCCCATGGCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTg                              <  7:92649/146‑1 (MQ=255)
                                                                                                                            ccaTCGAGCCCATGTCGAACGCAATGCGTTCGTGCT‑‑CTAGCTGGCCGGCATCGTAGTTGGACAAGTCATGAATCTCGTGCAGCAGCTCTACCAGCAGGTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGc                        <  5:136706/149‑1 (MQ=255)
                                                                                                                             caTCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCg                       >  4:129405/1‑149 (MQ=255)
                                                                                                                                      cATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTc                >  3:181141/1‑147 (MQ=255)
                                                                                                                                      cATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCa              <  7:345572/149‑1 (MQ=255)
                                                                                                                                       aTGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAg             >  8:295724/1‑149 (MQ=255)
                                                                                                                                         ctggAACGCATTGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCCGCGGGTTGTCCAgcg           >  4:39788/4‑149 (MQ=255)
                                                                                                                                          tCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAgcgc          >  6:125899/1‑149 (MQ=255)
                                                                                                                                             aaCGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAg       >  7:219616/1‑149 (MQ=255)
                                                                                                                                             aaCGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAg       >  7:40558/1‑149 (MQ=255)
                                                                                                                                              aCGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAgg      <  7:295724/149‑1 (MQ=255)
                                                                                                                                               cGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCCGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAggg     <  8:102056/149‑1 (MQ=255)
                                                                                                                                                 cAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAGGGCg   <  2:273362/149‑1 (MQ=255)
                                                                                                                                                  aaTGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAGGGCGa  >  6:134517/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGACGATCTGCCGCACACGCGTGGGGTCGCCGAGCACGCTGCTGGGGAAGTCGCTGGCGATCAGGCAGGTCAGCTCCACACTTTGCG‑CGGTGTTCTGCGACAGCAGGTTGGCGGTGTCTTCCACCATCGAGCCCATGTCGAACGCAATGCGTTCCAGCT‑‑CTAGCTGGCCGGCATCGAACTTGGACAAGTCAAGAATATCGTTCAGCAGCTCTACCAGCACCTTGCCCGAGTCATGGGCGATAGCCAGTTGCTGGCGTTGCTCGCTGGGCAGCGGGTTGTCCAGCGCCAGGGCGA  >  NC_002947/2098614‑2098907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑