Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,189,641	A→C	7.8%	N170H (AAC→CAC)	PP_1937 →	helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,189,641	0	A	C	7.8%	226.3 / 7.8	74	N170H (AAC→CAC)	PP_1937	helicase
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base C (4/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGTCACCTGGC  >  NC_002947/2189498‑2189779                                                                                                                                                |                                                                                                                                            aGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTg                                                                                                                                        <  8:78508/149‑1 (MQ=255)      aTTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTgcgc                                                                                                                                     <  7:154835/147‑1 (MQ=255)        tCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCa                                                                                                                                 >  7:331174/1‑149 (MQ=255)        tCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCa                                                                                                                                 >  2:115789/1‑149 (MQ=255)                  cACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTgggg                                                                                                                        >  5:21711/1‑148 (MQ=255)                     gTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGCACGTTCGCTTCatg                                                                                                                               <  3:169351/138‑3 (MQ=255)                     gTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGCACGTTCGCTTCatg                                                                                                                               >  4:169351/1‑136 (MQ=255)                          aaTTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGCACGTTCGCTTCatg                                                                                                                               >  6:172744/1‑131 (MQ=255)                          aaTTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGCACGTTCGCTTCatg                                                                                                                               <  5:172744/133‑3 (MQ=255)                               gTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCtt‑t                                                                                                         >  2:110825/1‑149 (MQ=255)                                   cccGTCAGTGTACAGACGGCTACATGCAAATATGTACTGCTCCGCTTGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      <  4:120766/148‑1 (MQ=255)                                   cccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      <  8:331174/148‑1 (MQ=255)                                   cccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      >  8:326439/1‑148 (MQ=255)                                   cccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      <  7:326439/148‑1 (MQ=255)                                    ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      >  2:99045/1‑147 (MQ=255)                                    ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      >  4:339345/1‑147 (MQ=255)                                    ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTga                                                                                                    <  1:99045/149‑1 (MQ=255)                                     cGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      >  8:234129/1‑146 (MQ=255)                                     cGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTgag                                                                                                   <  2:62495/149‑1 (MQ=255)                                       aCAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGAc                                                                                                 <  6:21711/149‑1 (MQ=255)                                         aGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTTACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCa                                                                                               <  5:355272/149‑1 (MQ=255)                                               cAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAAtctc                                                                                          <  1:115789/148‑1 (MQ=255)                                                aGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTcc                                                                                         >  4:388198/1‑148 (MQ=255)                                                 gACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTa                                                                                       <  4:192569/149‑1 (MQ=255)                                                  aCGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      >  3:5746/1‑133 (MQ=255)                                                  aCGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTt‑tgtt                                                                                                      <  4:5746/133‑1 (MQ=255)                                                  aCGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAg                                                                                      >  3:47588/1‑149 (MQ=255)                                                        aCATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGt                                                                                >  2:180391/1‑149 (MQ=255)                                                           tGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAgagg                                                                              >  5:59818/1‑145 (MQ=255)                                                           tGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCt                                                                             >  2:117142/1‑149 (MQ=255)                                                           tGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCt                                                                             >  7:5674/1‑149 (MQ=255)                                                             cAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGAGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCttt                                                                           >  2:113932/1‑149 (MQ=255)                                                              aaaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCtttt                                                                          >  2:78742/1‑149 (MQ=255)                                                              aaaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCtttt                                                                          >  6:299046/1‑149 (MQ=255)                                                              aaaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCtttt                                                                          >  7:122370/1‑149 (MQ=255)                                                                aaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTgg                                                                        <  5:413791/149‑1 (MQ=255)                                                                   gtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTgg                                                                        >  6:92993/4‑146 (MQ=255)                                                                   gtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTgg                                                                        <  5:92993/143‑1 (MQ=255)                                                                      aCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTg                                                                  >  6:38020/1‑149 (MQ=255)                                                                        tGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg                                                                <  8:220102/149‑1 (MQ=255)                                                                        tGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTCGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg                                                                >  2:178198/1‑149 (MQ=255)                                                                          cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTgggg                                                               <  3:339345/148‑1 (MQ=255)                                                                             cGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGgaga                                                            >  1:54315/1‑148 (MQ=255)                                                                             cGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGaa                                                           <  2:54315/149‑1 (MQ=255)                                                                             cGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGATGAGTGCTTTTGGAGCTTGGGgaga                                                            >  6:315694/1‑148 (MQ=255)                                                                                  gAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACg                                                        <  3:9/147‑1 (MQ=255)                                                                                    gCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCg                                                     >  6:392658/1‑148 (MQ=255)                                                                                       gTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGa                                                 <  5:299046/149‑1 (MQ=255)                                                                                         gACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCTCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGAc                                                <  4:38066/148‑1 (MQ=255)                                                                                          aCGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACg                                               <  1:180391/148‑1 (MQ=255)                                                                                            gCTTGGGTCCTGCAAGTACACTGGACCTGGTTTACTTACTCTGCATGATGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTTATCCCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCt                                             <  1:113932/148‑1 (MQ=255)                                                                                            gCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCtt                                            >  2:319575/1‑149 (MQ=255)                                                                                                   tCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGa                                     <  8:5674/149‑1 (MQ=255)                                                                                                     cAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAgc                                   <  4:246862/149‑1 (MQ=255)                                                                                                         aaCTACACTTGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAgctgct                               <  1:369921/149‑1 (MQ=255)                                                                                                           cTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGATCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGATAAACGCCGAAGACGCTTTCATTGAGCTGCTa                              >  7:373598/1‑148 (MQ=255)                                                                                                              cacTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTagag                           >  7:410438/1‑148 (MQ=255)                                                                                                              cacTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGagc                          <  3:388198/149‑1 (MQ=255)                                                                                                              cacTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGagc                          <  2:254943/149‑1 (MQ=255)                                                                                                                 tGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGagcagc                       >  6:59031/1‑149 (MQ=255)                                                                                                                 tGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGagcagc                       >  5:127734/1‑149 (MQ=255)                                                                                                                    aCCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTtc                    <  1:110825/149‑1 (MQ=255)                                                                                                                     ccAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTtct                   >  4:14772/1‑149 (MQ=255)                                                                                                                      cAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGTGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTtctc                  <  6:132382/149‑1 (MQ=255)                                                                                                                        ggTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTtctc                  >  2:428911/1‑147 (MQ=255)                                                                                                                        ggTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCaa                <  8:410438/149‑1 (MQ=255)                                                                                                                            tACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTGTGTTGAGACCAATCTCATAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGtt             >  1:120440/1‑149 (MQ=255)                                                                                                                             aCATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTc            <  7:234129/148‑1 (MQ=255)                                                                                                                             aCATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCg           >  4:129305/1‑149 (MQ=255)                                                                                                                              cATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGt          <  1:117142/149‑1 (MQ=255)                                                                                                                                    cAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGTCACCTg    >  6:411615/1‑149 (MQ=255)                                                                                                                                     aGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGTCACCTg    <  1:78742/148‑1 (MQ=255)                                                                                                                                        gtGAAGCGCACGTTCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGGTAGAGCAGCTTCTCAAGTTCGTCACCTGGc  >  5:179242/2‑147 (MQ=255)                                                                                                                                        gtGAAGCGCACGTTCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGTCACCTGGc  >  8:6953/2‑147 (MQ=255)                                                                                                                                                |                                                                                                                                            AGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTTACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTT‑TGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGGGAGAAACGCCGAAGACGCTTTCATTGAGCTGCTAGAGCAGCTTCTCAAGTTCGTCACCTGGC  >  NC_002947/2189498‑2189779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑