Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,695 G→C 9.2% T1067T (ACG→ACC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,6950GC 9.2% 291.1 / 10.8 96T1067T (ACG→ACC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (40/47);  new base C (6/3);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCC  >  NC_002947/197547‑197839
                                                                                                                                                       |                                                                                                                                                 
aaaCCCTTACCGGTGAAGCCGGCAAGACCCAGCGCAGCGTCGATTACCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAATTCGAGCAACTGACCCCGAACCCGA‑‑Cg                                                                                                                                                   <  8:145257/149‑1 (MQ=11)
 aaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGc                                                                                                                                                  <  6:369080/149‑1 (MQ=35)
   ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCg                                                                                                                                                <  5:134715/149‑1 (MQ=37)
    cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCgg                                                                                                                                               <  8:69885/149‑1 (MQ=37)
    cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCgg                                                                                                                                               >  8:346787/1‑149 (MQ=37)
    cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCgg                                                                                                                                               >  1:421247/1‑149 (MQ=37)
     aTTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGc                                                                                                                                              <  2:334666/149‑1 (MQ=37)
      ttACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCt                                                                                                                                             <  2:58168/149‑1 (MQ=12)
          cGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCAGa                                                                                                                                         >  3:212398/1‑149 (MQ=12)
           gTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGAc                                                                                                                                        >  6:189011/1‑149 (MQ=37)
            ttGAAGGCGTGAAAACCCAGGTGAGCGTCGACTGCCAGACCGCGGCGAATGACGTGTACA‑ACAACGGTTGGACTGTCAGCGTCAACATAGAGAACGCCACAGGCGGTAATTTCTATTAACTGACCCCGTACCCGT‑‑CCCCGGCTCAGAcc                                                                                                                                       <  8:302796/149‑1 (MQ=11)
            ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACACGA‑‑CGACGGCTCAGAcc                                                                                                                                       >  5:333512/1‑149 (MQ=255)
                aGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTACGACTGTCAGCCTCACCATCGAGAACGCCACACGCGCTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCAcaccacca                                                                                                                                   >  1:87932/1‑147 (MQ=11)
                 gccgccAAGACCCATGGCAGCTGCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGAt                                                                                                                                  <  8:156621/149‑1 (MQ=255)
                 gCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCAGACCACGa                                                                                                                                   >  2:407883/1‑148 (MQ=12)
                    ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCAc                                                                                                                               <  5:189011/149‑1 (MQ=38)
                     gCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACACCGAACCCGA‑‑CCCCGGCTCAGACACCGATCaac                                                                                                                              >  8:287451/1‑147 (MQ=11)
                       aaaaCCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAGACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCAGACCACGATCaac                                                                                                                              >  1:102667/4‑146 (MQ=11)
                       aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGa                                                                                                                            <  3:348061/149‑1 (MQ=39)
                              aGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTc                                                                                                                     <  6:60245/149‑1 (MQ=39)
                                ggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCg                                                                                                                    >  6:137184/1‑148 (MQ=39)
                                ggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCg                                                                                                                    <  5:137184/148‑1 (MQ=39)
                                     cGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGAcacca                                                                                                              >  8:353365/1‑149 (MQ=39)
                                      gTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGAcacca‑c                                                                                                            <  3:415834/149‑1 (MQ=39)
                                           tttCCAGACCCCGGCGAATGACGTCTACA‑ACTACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGAAATTTCGATCATCTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTg                                                                                                       <  4:281093/149‑1 (MQ=11)
                                            ttCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCa                                                                                                                                           >  7:282041/1‑113 (MQ=17)
                                            ttCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CCCCGGCTCa                                                                                                                                           <  8:282041/113‑1 (MQ=17)
                                             tcaaGACCCCTGCGAATGACGTTTACT‑ACTACGGTTCGACTGTCAGCGTCACGATCGAGAACGCGACAGGCGGTAATTTCGTGCATCTGTCCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGc                                                                                                      <  6:79898/145‑1 (MQ=14)
                                              ccAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGa                                                                                                    >  4:363621/1‑149 (MQ=255)
                                                aGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGAcc                                                                                                  <  1:29181/149‑1 (MQ=255)
                                                aGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGAcc                                                                                                  >  1:182978/1‑149 (MQ=255)
                                                 gACCCCGGCGAATGACGTCTACA‑ACAACGGCTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGAccc                                                                                                 <  8:175567/149‑1 (MQ=255)
                                                    cccGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCATGTGGTTTGAAACCTTATGCGCCGGCTCAGACCACGATCACCGACTCGGTCGACCCCA‑CTACTGCGAcac                                                                                                 >  1:358892/1‑146 (MQ=14)
                                                           atggtcGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGTGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCT‑CTACTGCGACCCTGACCGCCTc                                                                                       <  5:175158/144‑1 (MQ=255)
                                                             tGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGc                                                                                     >  7:262448/1‑149 (MQ=255)
                                                              gACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGc                                                                                     >  3:367456/1‑148 (MQ=255)
                                                               aCGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGcc                                                                                    >  7:322202/1‑148 (MQ=255)
                                                                 gTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAg                                                                                 >  6:240709/1‑149 (MQ=255)
                                                                  tCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGc                                                                                <  8:262448/149‑1 (MQ=255)
                                                                   cTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCg                                                                               >  3:11439/1‑149 (MQ=255)
                                                                   cTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACACCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCg                                                                               >  3:5497/1‑149 (MQ=255)
                                                                     aca‑acaACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCAAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGt                                                                              >  3:132238/1‑148 (MQ=255)
                                                                       a‑acaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGAc                                                                           >  7:247620/1‑149 (MQ=255)
                                                                         acaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGAcc                                                                          >  6:387576/1‑149 (MQ=255)
                                                                          caacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGAcc                                                                          >  8:61914/1‑148 (MQ=255)
                                                                              ggTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAgg                                                                     >  8:363580/1‑149 (MQ=255)
                                                                                ttCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcg                                                                   <  2:421247/149‑1 (MQ=255)
                                                                                   gACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcggc                                                                 <  7:346787/148‑1 (MQ=255)
                                                                                   gACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcggc                                                                 <  3:363621/148‑1 (MQ=255)
                                                                                   gACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGgcggcg                                                                >  2:75106/1‑149 (MQ=255)
                                                                                    aCTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACCTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGgcggcg                                                                <  1:90790/149‑1 (MQ=255)
                                                                                    aCTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCAAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGt                                                               <  4:132238/149‑1 (MQ=255)
                                                                                       gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGAt                                                            >  7:119864/1‑149 (MQ=255)
                                                                                        tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGAt                                                            >  1:1801/1‑148 (MQ=255)
                                                                                          aGCGGCACCTTCGAGTAAGCCAGAGGCTGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGCTCACCGACTCGGTCGACACGA‑CTACTGCGACCCTGACCGCCTCGCCATGCGTGACCGAAGGCGGCGTGATCAc                                                         <  8:311853/149‑1 (MQ=17)
                                                                                           gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAcc                                                        >  2:7153/1‑149 (MQ=255)
                                                                                            cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCt                                                       <  4:126724/149‑1 (MQ=255)
                                                                                               cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTaca                                                    >  6:156802/1‑149 (MQ=255)
                                                                                                 ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGCCCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACAGCCTCGCCAATCGTGACCGAAGGCGGCGTGATGACCTACAcc                                                  >  5:235808/1‑149 (MQ=255)
                                                                                                 ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACAcc                                                  >  1:317721/1‑149 (MQ=255)
                                                                                                  cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCg                                                 >  8:244357/1‑149 (MQ=255)
                                                                                                  cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCg                                                 >  4:304229/1‑149 (MQ=255)
                                                                                                    tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTg                                               <  7:164331/149‑1 (MQ=255)
                                                                                                      gagaACGCCACAGTCGGTAATTGCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACGGAAGGCGGCGTGATCACCTACACCGTGa                                              <  6:235808/148‑1 (MQ=255)
                                                                                                        gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGAccc                                           >  6:64522/1‑149 (MQ=255)
                                                                                                           cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa                                        <  5:373848/149‑1 (MQ=255)
                                                                                                           cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa                                        <  7:244357/149‑1 (MQ=255)
                                                                                                           cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa                                        <  8:322202/149‑1 (MQ=255)
                                                                                                           cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa                                        <  5:240709/149‑1 (MQ=255)
                                                                                                            gCCACAGGCTGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg                                       <  4:11439/149‑1 (MQ=255)
                                                                                                            gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg                                       >  8:208761/1‑149 (MQ=255)
                                                                                                            gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg                                       <  2:370019/149‑1 (MQ=255)
                                                                                                              cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCTGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg                                       <  4:5497/147‑1 (MQ=255)
                                                                                                              cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGc                                      >  8:264611/1‑148 (MQ=255)
                                                                                                                caGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAAt                                   <  2:1801/149‑1 (MQ=255)
                                                                                                                   gCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcggc                                                                 <  3:324979/116‑1 (MQ=255)
                                                                                                                   gCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcggc                                                                 >  4:324979/1‑116 (MQ=255)
                                                                                                                     ggTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGc                              <  7:353365/149‑1 (MQ=255)
                                                                                                                       tAATTTCGAGCAACTGACACCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGAGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc                             >  2:134170/1‑148 (MQ=255)
                                                                                                                          tttCGAGCAACTGACCCCGAACCAGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCATAGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACAACGTGCCCCTGAGCAATCATGCCCAGa                         >  4:275947/1‑149 (MQ=17)
                                                                                                                              gAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                     <  2:182978/149‑1 (MQ=255)
                                                                                                                              gAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                     >  1:22362/1‑149 (MQ=255)
                                                                                                                               aGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg                    <  2:358892/149‑1 (MQ=255)
                                                                                                                               aGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg                    <  3:304229/149‑1 (MQ=255)
                                                                                                                                 cAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt                  <  4:367456/149‑1 (MQ=255)
                                                                                                                                  aaCTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt                  <  7:61914/148‑1 (MQ=255)
                                                                                                                                    cTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAAc               >  2:395739/1‑149 (MQ=255)
                                                                                                                                       aCCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACAcc                                                  <  1:6372/111‑1 (MQ=255)
                                                                                                                                       aCCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACAcc                                                  >  2:6372/1‑111 (MQ=255)
                                                                                                                                        ccccGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTg           <  7:363580/149‑1 (MQ=255)
                                                                                                                                         cccGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGa          <  2:22284/149‑1 (MQ=255)
                                                                                                                                           cGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAcc        <  8:247620/149‑1 (MQ=255)
                                                                                                                                               cccGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGt    <  5:64522/149‑1 (MQ=255)
                                                                                                                                               cccGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGt    <  7:264611/149‑1 (MQ=255)
                                                                                                                                                ccGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGt    >  8:313058/1‑148 (MQ=255)
                                                                                                                                                 cGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTcc  <  7:313058/149‑1 (MQ=255)
                                                                                                                                                  gA‑‑CGCCTGCTCAGACCACGATCACCGACTCGGTCGACACCC‑CTACTGCGACCCTGACCCCCTCCCCAAGCGTGACCCAAGGCGGCGTGATCACCTCCACCGTGACCCTCAGCAATCCTGCCCCGACGCCGGTAACCGTGACCTTGTcc  >  6:354430/1‑148 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACA‑ACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGA‑‑CGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCA‑CTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCC  >  NC_002947/197547‑197839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑