Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,224,834 A→G 6.0% I600T (ATT→ACT)  PP_1963 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,224,8340AG 6.0% 189.8 / 2.5 66I600T (ATT→ACT) PP_1963hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/34);  new base G (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

CGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTTTC  >  NC_002947/2224689‑2224982
                                                                                                                                                 |                                                                                                                                                    
cGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAatat                                                                                                                                                   <  5:20988/149‑1 (MQ=255)
  gggCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGa                                                                                                                                                 <  4:22704/149‑1 (MQ=255)
      gTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCtg                                                                                                                                             <  3:344611/149‑2 (MQ=255)
       ttCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCtgg                                                                                                                                            <  5:212927/149‑3 (MQ=255)
        tCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGAGCTGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGa                                                                                                                                           >  3:21935/1‑149 (MQ=255)
              gTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTcc                                                                                                                                     <  5:271920/149‑1 (MQ=255)
                   tatCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCa                                                                                                                                 >  4:241179/1‑148 (MQ=255)
                   tatCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCa                                                                                                                                 >  2:116780/1‑148 (MQ=255)
                     tCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCaaa                                                                                                                               >  7:68166/1‑148 (MQ=255)
                       gcgAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATa                                                                                                                             <  6:287261/148‑1 (MQ=255)
                         gAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAggg                                                                                                                          <  5:169268/149‑1 (MQ=255)
                           aGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTa                                                                                                                        >  5:204717/1‑149 (MQ=255)
                             ccTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                      <  1:227286/149‑1 (MQ=255)
                               tGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGa                                                                                                                    >  3:157574/1‑149 (MQ=255)
                                 cTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGa                                                                                                                    <  2:291870/147‑1 (MQ=255)
                                    gATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCtt                                                                                                               >  2:262709/1‑149 (MQ=255)
                                     aTGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTc                                                                                                              <  5:388832/149‑1 (MQ=255)
                                        gtgtCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGt                                                                                                            <  5:123636/148‑1 (MQ=255)
                                               catcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAAtt                                                                                                       <  4:317591/146‑1 (MQ=255)
                                               catcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTc                                                                                                      <  1:116780/147‑1 (MQ=255)
                                               catcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCg                                                                                                     <  6:163845/148‑1 (MQ=255)
                                                atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGaa                                                                                                   >  5:95232/1‑149 (MQ=255)
                                                 tcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAAc                                                                                                  >  7:198171/1‑149 (MQ=255)
                                                 taatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTAAGAGTACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGAAGCTCAATCAAATAGGGTATTGAGTATTCGTAAATTCGaa                                                                                                   >  7:203709/3‑148 (MQ=255)
                                                  catGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAAc                                                                                                  >  2:384039/1‑148 (MQ=255)
                                                     gATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                              >  7:61481/1‑149 (MQ=255)
                                                      aTTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                              >  1:226399/1‑148 (MQ=255)
                                                       ttGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                            <  4:157574/149‑1 (MQ=255)
                                                       ttGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGATCCATCAAATAGCGTAGTGAGTCTTCGTAAATTCGAACCTgg                                                                                              >  8:266466/1‑147 (MQ=255)
                                                          tCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGagca                                                                                         <  6:204717/149‑1 (MQ=255)
                                                            gTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGagcagc                                                                                       >  3:173367/1‑149 (MQ=255)
                                                            gTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGagcagc                                                                                       <  1:262709/149‑1 (MQ=255)
                                                                 tgaGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATg                                                                                  <  8:61481/149‑1 (MQ=255)
                                                                  gagaAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATg                                                                                  >  8:389668/1‑148 (MQ=255)
                                                                     aaaCTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACt                                                                              <  1:30171/149‑1 (MQ=255)
                                                                        cTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGt                                                                           <  2:115664/149‑1 (MQ=255)
                                                                          ccAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                      <  5:259877/104‑1 (MQ=255)
                                                                          ccAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                      >  6:259877/1‑104 (MQ=255)
                                                                           cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTa                                                                        >  3:31091/1‑149 (MQ=255)
                                                                            aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTAt                                                                       >  2:207865/1‑149 (MQ=255)
                                                                                   tACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTa                                                                 <  2:226399/148‑1 (MQ=255)
                                                                                       ttCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAtt                                                            >  4:397794/1‑149 (MQ=255)
                                                                                         cccAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAttt                                                           <  3:397794/148‑1 (MQ=255)
                                                                                             gcgcAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAtttctttc                                                      <  1:44167/149‑1 (MQ=255)
                                                                                              cgcAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTcc                                                     <  1:384039/149‑1 (MQ=255)
                                                                                                 aGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTATTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCAtt                                                  <  7:389668/149‑1 (MQ=255)
                                                                                                  ggTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTa                                                 <  8:68166/149‑1 (MQ=255)
                                                                                                  ggTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTa                                                 <  4:166130/149‑1 (MQ=255)
                                                                                                       gagGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAAc                                            <  6:95232/149‑1 (MQ=255)
                                                                                                       gagGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAAc                                            >  8:176278/1‑149 (MQ=255)
                                                                                                        agGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAAc                                            <  1:122232/148‑1 (MQ=255)
                                                                                                        agGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAAc                                            >  2:122232/1‑148 (MQ=255)
                                                                                                          gACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGa                                         <  6:274446/149‑1 (MQ=255)
                                                                                                              gTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGc                                     >  5:215367/1‑149 (MQ=255)
                                                                                                                 cACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGCGCTAGACGCTCCATCAACTAGGGTAGTGAGGCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTc                                   <  4:21935/148‑1 (MQ=255)
                                                                                                                 cACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTc                                   <  3:241179/148‑1 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCAt                                                   <  4:56033/131‑1 (MQ=255)
                                                                                                                  aCCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCAt                                                   >  3:56033/1‑131 (MQ=255)
                                                                                                                    cTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAAt                               <  8:198171/149‑1 (MQ=255)
                                                                                                                    cTTCGCTTTCATTTTCCAGCTCATAATCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGGCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAAt                               <  8:203709/149‑1 (MQ=255)
                                                                                                                           ttCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTc                         >  1:394064/1‑148 (MQ=255)
                                                                                                                                     aGCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAGCCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTTGGAACCGATTGCTCGAATCCCTTCCAGTCTGGtt               >  8:380735/1‑148 (MQ=255)
                                                                                                                                      gCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGa             >  1:107216/1‑149 (MQ=255)
                                                                                                                                       cTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGa             >  1:235466/1‑148 (MQ=255)
                                                                                                                                          aTACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATAc          <  7:176278/148‑1 (MQ=255)
                                                                                                                                           tACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTAAGCTGTAGCATTTCTTTCGATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGATGATACt         >  3:425938/1‑148 (MQ=255)
                                                                                                                                             cTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAt      >  4:322761/1‑149 (MQ=255)
                                                                                                                                                aaTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGAAGACGATGCACTTGTATATGCTGTACCATTTCTTTCCATTAGGAACCGAATGCTCGAATCCCTTCAAGTCTGCTTGATACTGAtttt   >  5:180572/1‑149 (MQ=255)
                                                                                                                                                 atatGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTTTc  <  6:215367/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTTTC  >  NC_002947/2224689‑2224982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑