| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,273,080 | C→T | 13.4% | intergenic (+45/+246) | PP_2003 → / ← PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,273,080 | 0 | C | T | 13.4% | 127.0 / 9.6 | 52 | intergenic (+45/+246) | PP_2003/PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (20/25); new base T (3/4); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
ATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCA > NC_002947/2272932‑2273226 | aTGACTGGCAGGGTTGGGAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGc < 6:268121/149‑1 (MQ=255)aTGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGc < 5:13874/149‑1 (MQ=255)aTGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGc > 3:156454/1‑149 (MQ=255) gCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTc < 3:100211/149‑1 (MQ=255) aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAgg < 4:156454/149‑1 (MQ=255) aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAgg < 2:114079/149‑1 (MQ=255) gtgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATcccc > 7:213487/1‑149 (MQ=255) tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCt < 3:315733/149‑1 (MQ=255) cgCACCCATTCCGGGGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGAt < 2:299715/148‑1 (MQ=255) cACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGTCAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATa < 1:342245/149‑1 (MQ=255) ccATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCACTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTAATGCTGGAAACAACCCCAGTTACAGACGCCCCGCGATAACtt > 2:327743/1‑149 (MQ=255) cAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTg < 5:353131/148‑1 (MQ=255) aGGTTCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGCGGCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGTCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGaa < 5:316633/149‑1 (MQ=255) aGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCATTCGCCCG‑CAGCCC‑GCCAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACATTCTGaa > 5:344205/1‑149 (MQ=255) ggTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAac < 1:425891/149‑1 (MQ=255) agGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTa > 3:53466/1‑149 (MQ=255) gATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAAGCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACg < 4:360009/149‑1 (MQ=255) ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc > 5:185566/1‑149 (MQ=255) cAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCcgc < 7:344550/118‑1 (MQ=255) ccggccgAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACt < 4:3162/149‑1 (MQ=255) ccgAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCCCCCCTTTCGCCGG‑CCCCCC‑GCGAAAGGGCCCGTTCAGGGGGTTAACATCCCCAGTTACACACGCCCCGCCATAACAGTAAGAACACCAGGAACCTTTTCCACACAt > 7:204916/1‑148 (MQ=255) gggCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTTGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 5:176465/148‑1 (MQ=255) aGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 5:116623/149‑1 (MQ=255) gccgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCa > 4:99934/1‑142 (MQ=255) gccgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCa < 3:99934/142‑1 (MQ=255) cctccctTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTAATAAGCTTGTcataata > 5:237966/1‑147 (MQ=255) cctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATcc < 4:53466/149‑1 (MQ=255) tccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt > 1:329685/1‑149 (MQ=255) tCGCCTGCACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGc < 6:344205/149‑1 (MQ=255) ccTGAACCGACCCTTTCGCCGG‑CAGCCCGGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATc > 1:26772/1‑139 (MQ=255) ccTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCTAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTa < 8:213487/149‑1 (MQ=255) ccTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATc < 2:26772/138‑1 (MQ=255) cTGAACCGCCCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg > 5:444755/1‑149 (MQ=255) aaCCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAgg < 2:414645/147‑1 (MQ=255) aaCCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCGTAGGCt > 3:271852/1‑149 (MQ=255) ccGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg > 8:298030/1‑148 (MQ=255) cggcccTTTCGCGGG‑CTGCCG‑GCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg > 6:220352/4‑147 (MQ=39) cggcccTTTCGCGGG‑CTGCCG‑GCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg > 6:252082/4‑147 (MQ=39) gACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt < 4:319256/125‑1 (MQ=255) gACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt > 3:319256/1‑125 (MQ=255) gACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg > 3:362307/1‑149 (MQ=255) gcccTTTCGCGGG‑CTGCCG‑GCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAgg > 2:232209/2‑149 (MQ=255) cTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCg > 1:318809/1‑148 (MQ=255) tttCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa > 3:259030/1‑148 (MQ=255) gCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCt < 4:259030/149‑1 (MQ=255) cggg‑CTGCCG‑GCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTa < 5:220352/147‑1 (MQ=255) gCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAgg < 4:271852/149‑1 (MQ=255) ccggcgAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAg < 5:252082/144‑1 (MQ=255) cc‑GCGAAAGGGCAGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGc < 1:327743/148‑1 (MQ=255) gcgAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCaa > 2:105535/1‑148 (MQ=255) gcgAAAGGGCCGGTTCAGGCAGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCaaa < 6:45504/149‑1 (MQ=255) aaGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGa < 1:232209/149‑1 (MQ=255) ggCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc > 7:156149/1‑84 (MQ=255) ggCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 8:156149/84‑1 (MQ=255) gCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCa > 6:130987/1‑148 (MQ=255) | ATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGG‑CAGCCC‑GCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCA > NC_002947/2272932‑2273226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |