Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,290,396 C→T 8.1% intergenic (+21/‑254) pepN → / → PP_2018 aminopeptidase N/BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,290,3960CT 8.1% 138.2 / 2.1 49intergenic (+21/‑254)pepN/PP_2018aminopeptidase N/BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CTGACACGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGACCCACCA  >  NC_002947/2290250‑2290542
                                                                                                                                                  |                                                                                                                                                  
cTGACACGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCa                                                                                                                                                  <  1:121289/149‑1 (MQ=255)
   acacGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCg                                                                                                                                               <  4:98998/149‑1 (MQ=255)
   acacGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCg                                                                                                                                               <  2:367927/149‑1 (MQ=255)
     acGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGc                                                                                                                                             >  1:128420/1‑149 (MQ=255)
          ggCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCTGGGCGGccccaa                                                                                                                                        <  7:215139/149‑3 (MQ=255)
            cgcAAGTATGACGATGCGCGTCAGGCGCTGCTGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCt                                                                                                                                      <  8:402838/149‑1 (MQ=255)
               aaGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTc                                                                                                                                   >  7:163111/1‑149 (MQ=255)
                    tGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCgg                                                                                                                              <  1:393421/149‑1 (MQ=255)
                     gACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTcc                                                                                                                                  <  3:158980/144‑1 (MQ=255)
                     gACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTcc                                                                                                                                  >  4:158980/1‑144 (MQ=255)
                      aCGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTCTGTTTGAGTTGTATTGTTATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGc                                                                                                                             <  1:142534/148‑1 (MQ=255)
                        gATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGa                                                                                                                          <  8:232417/149‑1 (MQ=255)
                              cgTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGAcaacaa                                                                                                                    <  4:394382/149‑1 (MQ=255)
                                tCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGTCCCCGGCTTTCCCCGGCCGACAACaaaa                                                                                                                  <  2:169001/149‑1 (MQ=255)
                                         gatgaAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGTCCCGGCTTTCCCCGGCCGACAACAAAACATAACAcc                                                                                                         >  5:164300/1‑149 (MQ=255)
                                                      cTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACa                                                                                                                >  6:348552/1‑129 (MQ=255)
                                                      cTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACa                                                                                                                <  5:348552/129‑1 (MQ=255)
                                                        ggAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTc                                                                                          >  2:224785/1‑149 (MQ=255)
                                                         gtccgaaTTGTGGCTTGGTGTGAGTTGTCCATTCTTGGTTATGAGGTTGTTTGCAAGAGTCGGGGGTAATTCTGGGTATTTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTc                                                                                          <  4:22030/142‑1 (MQ=255)
                                                                ttCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCaaa                                                                                   >  6:7564/1‑148 (MQ=255)
                                                                   tGGGTTCGGGTGAGTTGTCCAGGGATGGGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATc                                                                               <  8:163111/149‑1 (MQ=255)
                                                                                 tgggccAGTGATGTGTATGAGGTCGTGAGCAAGAGTCTGGCGTAATTCTGCTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGAtctc                                                                 <  4:83144/145‑1 (MQ=255)
                                                                                   gTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATctcc                                                                <  5:7564/148‑1 (MQ=255)
                                                                                       aGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGTCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAg                                                           <  6:164300/149‑1 (MQ=255)
                                                                                             gtgtATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTg                                                     >  8:66709/1‑149 (MQ=255)
                                                                                                  tGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGc                                                <  2:128420/149‑1 (MQ=255)
                                                                                                  tGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGATACGATCTCCTCAAGCTATTGCAGGc                                                >  2:186226/1‑149 (MQ=255)
                                                                                                   gAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGcc                                               >  7:208143/1‑149 (MQ=255)
                                                                                                   gAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGc                                                <  8:94671/148‑1 (MQ=255)
                                                                                                      gTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCctc                                             >  8:389323/1‑148 (MQ=255)
                                                                                                      gTTGTGAGCAAGAGTCTGGCGTAATTCTAGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCGGGCctc                                             >  7:334959/1‑148 (MQ=255)
                                                                                                         gtgAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCctc                                             >  3:169158/1‑145 (MQ=255)
                                                                                                           gAGCAATTGTCTGGCGTAATTCTGGTGATTTGTGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAATCATAACACCCCGCCCGTTGTACTCGCGCAATCATCCCCGCTACGATCTCCTCAAGCTATTg                                                     <  4:31443/135‑1 (MQ=255)
                                                                                                            aGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCa                                      <  5:264264/149‑1 (MQ=255)
                                                                                                             gCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATcc                                                                              >  4:24377/1‑108 (MQ=255)
                                                                                                             gCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATcc                                                                              <  3:24377/108‑1 (MQ=255)
                                                                                                                   gTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTc                               <  2:39813/149‑1 (MQ=255)
                                                                                                                    tCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCt                              >  2:112786/1‑149 (MQ=255)
                                                                                                                          gTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAg                        >  2:274422/1‑149 (MQ=255)
                                                                                                                                 tGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCaa                 >  4:130380/1‑149 (MQ=255)
                                                                                                                                    tGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATg               >  2:397865/1‑148 (MQ=255)
                                                                                                                                      aaTTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCa             <  1:229665/148‑1 (MQ=255)
                                                                                                                                        ttGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCAtt           >  3:239153/1‑148 (MQ=255)
                                                                                                                                        ttGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCAtt           >  5:65364/1‑148 (MQ=255)
                                                                                                                                          ggggCCGCTGGGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGc              >  1:304085/1‑143 (MQ=255)
                                                                                                                                          ggggCCGCTGGGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGc              <  2:304085/143‑1 (MQ=255)
                                                                                                                                          ggggCCGCTGGGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGAc        >  7:340357/1‑149 (MQ=255)
                                                                                                                                          ggggCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGa         <  4:203432/148‑1 (MQ=255)
                                                                                                                                          ggggCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTACATCAGGCTTTCTGCAAAGCCTGCAATGCATTGa         <  1:224785/148‑1 (MQ=255)
                                                                                                                                              ccgccCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCGGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGACccac    <  8:334959/149‑1 (MQ=255)
                                                                                                                                                 cccAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGACccacca  >  7:57950/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGACACGCTGGCGCAAGTATGACGATGCGCGTCAGGCGCTGATGAAGGGTGAGCTGGAGCGCATTCTGGCTTCGGGTGAGTTGTCCAGTGATGTGTATGAGGTTGTGAGCAAGAGTCTGGCGTAATTCTGGTGAATTGGGGCCGCCCAGCGGCCCCGGCTTTCCCCGGCCGACAACAAAACATAACACCCCGCCCGTTGTACTCCCGCAAACATCCCCGCTACGATCTCCTCAAGCTATTGCAGGCCTCTAGATCAGGCTTTCTGCAAAGCCTGCAATGCATTGACCCACCA  >  NC_002947/2290250‑2290542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑