Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,313,687	A→T	6.2%	I134N (ATT→AAT)	benE‑I ←	benzoate transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,313,687	0	A	T	6.2%	195.9 / 2.3	64	I134N (ATT→AAT)	benE‑I	benzoate transport protein
Reads supporting (aligned to +/- strand):  ref base A (27/33);  new base T (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGCGGCTGGCGT  >  NC_002947/2313548‑2313835                                                                                                                                             |                                                                                                                                                     cACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCgg                                                                                                                                             <  2:417215/149‑1 (MQ=255) cACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCgg                                                                                                                                             >  2:166426/1‑149 (MQ=255)  aCGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCCAGCAGGCCGGCGGCAATGCCGCCgg                                                                                                                                             <  1:19732/149‑1 (MQ=255)  aCGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCggg                                                                                                                                            <  1:111910/149‑1 (MQ=255)  aCGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCggg                                                                                                                                            <  6:149899/149‑1 (MQ=255)            gggCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTAcgcg                                                                                                                                   >  1:251660/1‑148 (MQ=255)            gggCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACgcgc                                                                                                                                  <  4:401402/149‑1 (MQ=255)                        gTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCg                                                                                                                      <  7:222759/149‑1 (MQ=255)                         tttGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCg                                                                                                                      >  1:239912/1‑148 (MQ=255)                          ttGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCg                                                                                                                      >  7:393320/1‑147 (MQ=255)                             aaaGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGc                                                                                                                  <  8:393320/148‑1 (MQ=255)                              actccACAGACGCTGCAATCAGGCACCCGGCGTGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCGGGCGGCAAAGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCAc                                                                                                                <  8:399784/146‑1 (MQ=255)                              aaGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCAc                                                                                                                >  2:263069/1‑149 (MQ=255)                                     aTACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCt                                                                                                           >  5:298189/1‑147 (MQ=255)                                       aCGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGc                                                                                                       >  4:158823/1‑149 (MQ=255)                                        cGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGc                                                                                                       <  4:10686/148‑1 (MQ=255)                                         gCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGccc                                                                                                     <  2:279917/149‑1 (MQ=255)                                          cTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGccc                                                                                                     >  2:110568/1‑148 (MQ=255)                                             cAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCa                                                                                                   <  6:298189/147‑1 (MQ=255)                                             cAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCa                                                                                                   <  1:68107/147‑1 (MQ=255)                                                  aGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcaccac                                                                                            <  2:292366/149‑1 (MQ=255)                                                   gCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcaccac                                                                                            >  3:159627/1‑148 (MQ=255)                                                    cAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACg                                                                                           >  7:119561/1‑148 (MQ=255)                                                      aCCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAg                                                                                        >  4:226671/1‑149 (MQ=255)                                                       cccGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGc                                                                                       <  1:203672/149‑1 (MQ=255)                                                              aGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCggcggc                                                                                <  2:280015/149‑1 (MQ=255)                                                                 cTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGa                                                                             <  1:196639/149‑1 (MQ=255)                                                                  tGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGa                                                                             >  3:395180/1‑148 (MQ=255)                                                                    gggTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGAcag                                                                          >  3:33134/1‑149 (MQ=255)                                                                       tCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcac                                                                                               >  7:78624/1‑125 (MQ=255)                                                                       tCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcac                                                                                               <  8:78624/125‑1 (MQ=255)                                                                         gaccgacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAgg                                                                      <  1:166426/148‑1 (MQ=255)                                                                         gaccgacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAgg                                                                      >  3:52983/1‑148 (MQ=255)                                                                            cgacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGt                                                                     >  5:169975/1‑146 (MQ=255)                                                                            cgacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGt                                                                     <  6:169975/146‑1 (MQ=255)                                                                              acAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCa                                                                >  4:93866/1‑149 (MQ=255)                                                                                aGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCAcc                                                              >  4:186600/1‑149 (MQ=255)                                                                                 ggCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCAcc                                                              >  5:53416/1‑148 (MQ=255)                                                                                    tGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATg                                                          >  8:410252/1‑149 (MQ=255)                                                                                      gCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCCCGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGc                                                        >  7:268092/1‑149 (MQ=255)                                                                                            gCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCt                                                  <  1:110568/149‑1 (MQ=255)                                                                                            gCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCt                                                  >  4:45207/1‑149 (MQ=255)                                                                                             cACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTg                                                 <  2:239912/149‑1 (MQ=255)                                                                                             cACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTg                                                 <  3:45207/149‑1 (MQ=255)                                                                                               ccGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTa                                               <  6:288473/149‑1 (MQ=255)                                                                                                      cGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACg                                        <  4:159627/149‑1 (MQ=255)                                                                                                                cagGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAggg                              <  7:410252/149‑1 (MQ=255)                                                                                                                    aTGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGt                          <  1:263069/149‑1 (MQ=255)                                                                                                                       ccGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGa                        <  8:119561/148‑1 (MQ=255)                                                                                                                       ccGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGa                        <  2:251660/148‑1 (MQ=255)                                                                                                                        cGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAcc                      <  3:199132/149‑1 (MQ=255)                                                                                                                          g‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAcc                      >  3:244941/1‑147 (MQ=255)                                                                                                                            ccAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAg                   <  4:52983/149‑1 (MQ=255)                                                                                                                                cagGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGGCTCGCTGTAGGGCACGCTGGCCAg                                >  5:370339/1‑132 (MQ=255)                                                                                                                                cagGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAg                                <  6:370339/132‑1 (MQ=255)                                                                                                                                 tgctggccggcAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAAcgc              >  6:381076/8‑149 (MQ=255)                                                                                                                                 agGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAAcgc              <  3:185811/149‑1 (MQ=255)                                                                                                                                   gCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGaa                                                                                                                    <  1:55874/45‑1 (MQ=255)                                                                                                                                   gCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGaa                                                                                                                    >  2:55874/1‑45 (MQ=255)                                                                                                                                   cccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGaa                 <  7:11321/143‑1 (MQ=255)                                                                                                                                   cccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGaa                 >  8:11321/2‑144 (MQ=255)                                                                                                                                    ccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGc                             >  1:209264/1‑131 (MQ=255)                                                                                                                                    ccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGc                             <  2:209264/131‑1 (MQ=255)                                                                                                                                          gcAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGCGGCTgg     >  2:258775/1‑149 (MQ=255)                                                                                                                                            aaTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGCGGCTGGCg   <  5:381076/149‑1 (MQ=255)                                                                                                                                             aTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGCGGCTGGCGt  <  4:33134/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                     CACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGG‑CCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGCGGCTGGCGT  >  NC_002947/2313548‑2313835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑