Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,335,609 A→C 6.7% M283R (ATG→AGG) ‡ PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,335,6090AC 6.7% 267.3 / 7.6 86M283R (ATG→AGG) ‡PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (43/37);  new base C (2/4);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

CGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAACGGCTCGC  >  NC_002947/2335463‑2335756
                                                                                                                                                  |                                                                                                                                                    
cGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATg                                                                                                                                                    <  7:100647/149‑1 (MQ=255)
     atTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATgcgc                                                                                                                                                 >  7:382218/1‑147 (MQ=255)
      tttCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAg                                                                                                                                              >  2:126094/1‑149 (MQ=255)
        tCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCATGCTGCGCGaaa                                                                                                                                             <  3:213599/148‑3 (MQ=255)
        tCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCATGCTGCGCGaaa                                                                                                                                             <  2:363630/148‑3 (MQ=255)
          ggCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCggg                                                                                                                                          >  5:97442/1‑149 (MQ=255)
           gCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCATGCTGCGCGaaagcgt                                                                                                                                         <  5:251249/149‑7 (MQ=255)
              gtggtgTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGc                                                                                                                                      >  1:181176/1‑149 (MQ=255)
              gtggtgTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGc                                                                                                                                      <  5:235713/149‑1 (MQ=255)
                 gtgtACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCggg                                                                                                                                          <  6:87650/142‑1 (MQ=255)
                 gtgtACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCggg                                                                                                                                          >  5:87650/1‑142 (MQ=255)
                    tACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCaa                                                                                                                                 <  7:351616/148‑1 (MQ=255)
                       aTGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGAt                                                                                                                             <  5:105319/149‑1 (MQ=255)
                          cTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGAttt                                                                                                                           >  1:387965/1‑148 (MQ=255)
                             atgaCCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCAcg                                                                                                                       >  5:346373/1‑149 (MQ=255)
                             atgaCCGCGGCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCTCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCAc                                                                                                                        <  6:383800/148‑1 (MQ=255)
                               gaCCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCAcgcg                                                                                                                     >  4:390173/1‑149 (MQ=255)
                                aCCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCgg                                                                                                                    >  7:297351/1‑149 (MQ=255)
                                  cgcgTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTc                                                                                                                  >  8:290031/1‑149 (MQ=255)
                                  cgcgTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTc                                                                                                                  <  5:295604/149‑1 (MQ=255)
                                    cgtcgtcgtcgtCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCgg                                                                                                                <  2:424956/149‑1 (MQ=255)
                                       cgtcgtcgtcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGgtg                                                                                                             >  2:135318/1‑149 (MQ=255)
                                        gtcgtcttcAATGTCTATCATGCGCAGGTTGTCGATGGTCTGGTCGAACGACTTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGgtg                                                                                                             <  6:375036/148‑1 (MQ=255)
                                         tcgtcgtcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTa                                                                                                           <  4:101890/149‑1 (MQ=255)
                                           gtcgtcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGc                                                                                                         >  2:296913/1‑149 (MQ=255)
                                             cgtcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGAATTCACGCGGTCTGGTGTAGCCg                                                                                                       >  6:183324/1‑149 (MQ=255)
                                              gtcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGt                                                                                                      >  3:85993/1‑149 (MQ=255)
                                               tcCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTc                                                                                                     >  4:83786/1‑149 (MQ=255)
                                                 cATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTcc                                                                                                    >  8:22933/1‑148 (MQ=255)
                                                 cATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTcc                                                                                                    <  6:97442/148‑1 (MQ=255)
                                                     tCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATg                                                                                               <  4:396291/149‑1 (MQ=255)
                                                       gATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATgg                                                                                              >  4:234648/1‑148 (MQ=255)
                                                         tCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCTGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGa                                                                                            >  7:180947/1‑148 (MQ=255)
                                                           aTGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGaaa                                                                                          <  3:390173/148‑1 (MQ=255)
                                                            tGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGaaaa                                                                                         >  8:245835/1‑148 (MQ=255)
                                                               gcACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATAcc                                                                                     >  8:309974/1‑149 (MQ=255)
                                                                cACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCt                                                                                    >  2:306844/1‑149 (MQ=255)
                                                                cACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCt                                                                                    <  2:387965/149‑1 (MQ=255)
                                                                      gTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑c                                                                              <  7:290031/148‑1 (MQ=255)
                                                                       tCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAg                                                                            >  3:325639/1‑149 (MQ=255)
                                                                       tCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAg                                                                            >  4:189353/1‑149 (MQ=255)
                                                                         gATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAgg                                                                           >  2:426238/1‑148 (MQ=255)
                                                                         gATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAgg                                                                           >  3:412784/1‑148 (MQ=255)
                                                                             gtctggtcGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAAcc                                                                      >  7:360327/1‑149 (MQ=255)
                                                                                tggtcGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAAccgcc                                                                   >  8:262668/1‑149 (MQ=255)
                                                                                  gtcGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAAccgccgc                                                                 >  5:210184/1‑149 (MQ=255)
                                                                                  gtcGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAAccgccgc                                                                 >  1:239617/1‑149 (MQ=255)
                                                                                  gtcGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAAccgccgc                                                                 >  3:47837/1‑149 (MQ=255)
                                                                                     gAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGa                                                              >  5:379576/1‑149 (MQ=255)
                                                                                     gAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGa                                                              <  8:382218/149‑1 (MQ=255)
                                                                                      aaCGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGAt                                                             <  2:181176/149‑1 (MQ=255)
                                                                                       acgacgTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCAc                                                                                                                        >  7:25050/1‑90 (MQ=255)
                                                                                       acgacgTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCAc                                                                                                                        <  8:25050/90‑1 (MQ=255)
                                                                                       acgacgTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAAt                                                                                        >  2:58290/1‑122 (MQ=255)
                                                                                          acgTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGgcg                                                         <  2:239617/149‑1 (MQ=255)
                                                                                           cgTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGgcgc                                                        >  5:33019/1‑149 (MQ=255)
                                                                                               gCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGa                                                     >  3:317382/1‑148 (MQ=255)
                                                                                                cACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGaaa                                                   <  4:315035/149‑1 (MQ=255)
                                                                                                cACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGaaa                                                   <  1:426238/149‑1 (MQ=255)
                                                                                                   cGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCa                                                >  4:272130/1‑149 (MQ=255)
                                                                                                       gcgcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGaa                                            >  1:275939/1‑149 (MQ=255)
                                                                                                        cgcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAAt                                           <  4:412784/149‑1 (MQ=255)
                                                                                                        cgcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAAt                                           <  4:325639/149‑1 (MQ=255)
                                                                                                        cgcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAAt                                           <  8:297351/149‑1 (MQ=255)
                                                                                                        cgcACGCTGTAGCGGCAGATTGCTCTGACTCTTTCGCGCAGCATGCGCGAGCGGGTTGCTGCAATGATTTCACGCGTTCGGGTGTAGCCGTCCCAATTGGAAAATACCTTCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAAt                                           <  8:180947/149‑1 (MQ=255)
                                                                                                         gcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATg                                          <  1:135318/149‑1 (MQ=255)
                                                                                                         gcACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATg                                          <  4:47837/149‑1 (MQ=255)
                                                                                                               tGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACg                                    <  3:272130/149‑1 (MQ=255)
                                                                                                                      gCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACt                             <  4:85993/149‑1 (MQ=255)
                                                                                                                           aGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTc                        <  1:418666/149‑1 (MQ=255)
                                                                                                                               gcTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATc                     >  6:426244/1‑148 (MQ=255)
                                                                                                                               gcTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTCGTTCATc                     >  7:153526/1‑148 (MQ=255)
                                                                                                                                 tGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAg                  >  4:64892/1‑149 (MQ=255)
                                                                                                                                   aCGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGTCCCGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGa                   <  1:126094/147‑1 (MQ=255)
                                                                                                                                   aCGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAg                  <  2:350721/147‑1 (MQ=255)
                                                                                                                                     gCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCg              >  8:19563/1‑149 (MQ=255)
                                                                                                                                     gCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCg              >  2:340901/1‑149 (MQ=255)
                                                                                                                                     ccgctCGCGCATGCTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCg              >  7:317139/5‑149 (MQ=255)
                                                                                                                                      cgctCGCGCATGCTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCgg             >  3:252207/4‑149 (MQ=255)
                                                                                                                                      cTTTCGCGCAGCATGCTCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCgg             <  4:382947/149‑1 (MQ=255)
                                                                                                                                       tttCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCgg             >  4:73082/1‑148 (MQ=255)
                                                                                                                                          cgcgCAGCCTGCGCGAGCGGGGCGCGGCAATTATTGCACCCGGTCGGTGTTAGCGGTCCCAATGGTATAATCCGTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCTTCGAGGCCgtcaac         <  4:57749/149‑6 (MQ=255)
                                                                                                                                          cgcgCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGaa          <  7:262668/148‑1 (MQ=255)
                                                                                                                                          cgcgCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGaa          <  7:22933/148‑1 (MQ=255)
                                                                                                                                           gcgcAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAACg        <  5:183324/149‑1 (MQ=255)
                                                                                                                                           gcgcAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGGCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAAc         <  5:18820/148‑1 (MQ=255)
                                                                                                                                             gcagcaTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAACGgc      >  4:108022/1‑149 (MQ=255)
                                                                                                                                                 caTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGcc               >  4:148172/1‑136 (MQ=255)
                                                                                                                                                 caTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGcc               <  3:148172/136‑1 (MQ=255)
                                                                                                                                                 caTGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAACGgctcgc  >  6:328026/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCTTTCGCGCAGCATGCGCGAGCGGGTTGCGGCAATGATTTCACGCGGTCGGGTGTAGCCGTCCCAATGGGAAAATACCTGCGT‑CAGGTAACCGCCGCCGATGGCGCCGAAACCATGAATGCCCACGCTGAACTGGTTCATCGAGGCCGGGAACGGCTCGC  >  NC_002947/2335463‑2335756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑