Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,057 T→A 5.6% C258S (TGC→AGC) ‡ gapB → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,0570TA 5.6% 218.8 / 2.7 71C258S (TGC→AGC) ‡gapBglyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base A (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

CCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTGC  >  NC_002947/2455917‑2456204
                                                                                                                                            |                                                                                                                                                   
ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGt                                                                                                                                             >  7:297631/1‑149 (MQ=255)
  aaCGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtgc                                                                                                                                           >  4:405503/1‑149 (MQ=255)
    cGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtgctg                                                                                                                                         >  5:15977/1‑149 (MQ=255)
     ggCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctgg                                                                                                                                        <  5:156070/149‑2 (MQ=255)
        aaCCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgccca                                                                                                                                    <  7:23447/149‑3 (MQ=255)
           cTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGt                                                                                                                                  <  1:339829/149‑1 (MQ=255)
              aTCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGAt                                                                                                                               >  8:132153/1‑149 (MQ=255)
                    gTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCAc                                                                                                                         >  5:177409/1‑149 (MQ=255)
                       aTCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCg                                                                                                                       <  6:442689/148‑1 (MQ=255)
                           aCGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGATCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCt                                                                                                                  >  5:444935/1‑149 (MQ=255)
                               cAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGc                                                                                                               >  7:267898/1‑148 (MQ=255)
                                  gagCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAgg                                                                                                           <  7:270306/149‑1 (MQ=255)
                                     cccTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACAGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcg                                                                                                        >  8:30607/1‑149 (MQ=255)
                                       cTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcgcg                                                                                                      <  6:7802/149‑1 (MQ=255)
                                          gCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTg                                                                                                   <  8:297631/149‑1 (MQ=255)
                                           cGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACCCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTga                                                                                                  <  2:111563/149‑1 (MQ=255)
                                           cGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTg                                                                                                   >  3:53025/1‑148 (MQ=255)
                                            gAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaa                                                                                                 <  7:238756/149‑1 (MQ=255)
                                                tCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaagaa                                                                                              >  3:184546/1‑148 (MQ=255)
                                                   aCTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATc                                                                                          <  1:404200/149‑1 (MQ=255)
                                                    cTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCg                                                                                         <  6:408028/149‑1 (MQ=255)
                                                        acCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCa                                                                                     >  5:179028/1‑149 (MQ=255)
                                                        acCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCa                                                                                     >  4:251878/1‑149 (MQ=255)
                                                          ccAGTACGGCATCGACAATGCGCTCATTGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACg                                                                                   >  3:293840/1‑149 (MQ=255)
                                                          ccAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACg                                                                                   <  8:231549/149‑1 (MQ=255)
                                                            aGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGt                                                                                 <  7:30607/149‑1 (MQ=255)
                                                                  gCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGTACATCGTGCACGGTATCAAc                                                                           >  6:93607/1‑149 (MQ=255)
                                                                    aTCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCa                                                                         >  5:341040/1‑149 (MQ=255)
                                                                    aTCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCATGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCAGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCa                                                                         >  1:109922/1‑149 (MQ=255)
                                                                     tCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaa                                                                                                 <  4:370450/124‑1 (MQ=255)
                                                                     tCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaa                                                                                                 >  3:370450/1‑124 (MQ=255)
                                                                       gACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACgg                                                                      <  8:267898/149‑1 (MQ=255)
                                                                       gACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACgg                                                                      >  2:230840/1‑149 (MQ=255)
                                                                         cAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCgg                                                                                                                                               <  2:226435/74‑1 (MQ=255)
                                                                         cAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCgg                                                                                                                                               >  1:226435/1‑74 (MQ=255)
                                                                          aaTGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCCCCTGGCAAGGGCGCGCTGAAGACCCTCGTGCACGGTATCAACCACGGTGa                                                                   >  8:115641/1‑149 (MQ=255)
                                                                              cgcTCATTGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGatat                                                                <  4:293840/148‑1 (MQ=255)
                                                                                   aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGc                                                          <  3:179799/149‑1 (MQ=255)
                                                                                    tcgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGcc                                                         >  7:251963/1‑149 (MQ=255)
                                                                                    tcgtcgACAACAACGGCGTATGGCGTGAAGCAGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATACTCACCGCACCTGGCAAGGGCGCTCTGAAGAACAGCGTGCACGGTAGCAACCACGGTGCTATCACTGcc                                                         >  7:251965/1‑149 (MQ=255)
                                                                                     cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGcc                                                         >  5:39563/1‑148 (MQ=255)
                                                                                     cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGcc                                                         >  3:251098/1‑148 (MQ=255)
                                                                                       tcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGAt                                                      <  3:117583/149‑1 (MQ=255)
                                                                                         gACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGa                                                    >  6:336894/1‑149 (MQ=255)
                                                                                            atctccGGCGTATGGAGTGACGCCGACGGCCTGTGCCAGCACCTGGCCTGCCCGGGTGATGCCAGCGTGATCCTCACGTCACCTTTGAAGGGAGCGCTGAATAACATCGTGCAAGGTATCAACCACGGTGATATCTCTGCCTATGa                                                    <  6:398585/142‑1 (MQ=255)
                                                                                            aacaCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGa                                                    >  5:398585/1‑146 (MQ=255)
                                                                                              cacCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGa                                               <  3:251878/149‑1 (MQ=255)
                                                                                               acCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGAc                                                   >  6:443231/1‑144 (MQ=255)
                                                                                                   gCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGatcatc                                          <  7:279267/149‑1 (MQ=255)
                                                                                                   gCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGatcatc                                          >  8:279267/1‑149 (MQ=255)
                                                                                                            gTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACTAGATCATCTCGGCGGcc                                 <  4:303234/149‑1 (MQ=255)
                                                                                                             tGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGcct                                >  5:224931/1‑149 (MQ=255)
                                                                                                               aCGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGcctcc                              <  7:152603/149‑1 (MQ=255)
                                                                                                                 gCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCCCTGCCGATGACAAGATCATCTCGGCGGCCTCCTg                            >  6:415744/1‑149 (MQ=255)
                                                                                                                  ccGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGc                           <  1:230840/149‑1 (MQ=255)
                                                                                                                   cGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATAGCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGca                          <  3:386411/149‑1 (MQ=255)
                                                                                                                       ggCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGcaccac                      <  6:341040/149‑1 (MQ=255)
                                                                                                                            gggCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACg                 >  6:190214/1‑149 (MQ=255)
                                                                                                                             ggCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAAc                  <  3:405503/147‑1 (MQ=255)
                                                                                                                             ggCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAAc                  <  4:251098/147‑1 (MQ=255)
                                                                                                                             ggCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGc                >  2:224063/1‑149 (MQ=255)
                                                                                                                              ggcAGCACCCGGGCAGGCCAGGTGCTGGCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGc                >  2:377549/3‑148 (MQ=255)
                                                                                                                              ggcAGCACCCGGGCAGGCCAGGTGCTGCCCGCGTGATCCTCACCGCCCCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGc                >  8:390720/3‑148 (MQ=255)
                                                                                                                                 aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTTCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATc            <  2:278147/149‑1 (MQ=255)
                                                                                                                                 aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATc            <  6:177409/149‑1 (MQ=255)
                                                                                                                                    aCCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTg         >  5:238723/1‑149 (MQ=255)
                                                                                                                                      cTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGcc       >  7:174512/1‑149 (MQ=255)
                                                                                                                                      cTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGcc       <  5:336894/149‑1 (MQ=255)
                                                                                                                                        ggCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCCCCACCAACGCCATCGTGCCgg     >  2:157256/1‑149 (MQ=255)
                                                                                                                                          ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCTGCCTCCTGCACCACCAACGCCATCGTGCCGGTg   <  6:444935/149‑1 (MQ=255)
                                                                                                                                           cTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTGc  >  2:232676/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTGC  >  NC_002947/2455917‑2456204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑