| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,486,810 | A→G | 7.8% | intergenic (+43/‑141) | spuC‑I → / → PP_2181 | polyamine:pyruvate transaminase/Cro/CI family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,486,810 | 0 | A | G | 7.8% | 139.0 / 2.8 | 51 | intergenic (+43/‑141) | spuC‑I/PP_2181 | polyamine:pyruvate transaminase/Cro/CI family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (26/21); new base G (2/2); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
ACCATGATCATCGCGCCACCGCTGGTGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGAAAGT > NC_002947/2486663‑2486958 | acCATGATCATCGCGCCACCGCTGGTGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCaa < 5:61958/149‑1 (MQ=255) cATGATCATCGCGCCACCGCTGGTGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAg > 3:35726/1‑148 (MQ=255) gcCACCGCTGGTGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGcgcg < 5:227/149‑1 (MQ=255) gCTGTTCATGAGTATCGAAGAGAGCGACGTTCTGGGGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGATGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGATc < 8:146536/149‑1 (MQ=255) tGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGGCCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCGAGCACCGCGCAGGCGGTGCTTGATct < 3:259896/149‑2 (MQ=38) cAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTgc > 6:400229/1‑149 (MQ=255) tCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTgccgcc < 4:375795/148‑1 (MQ=255) cGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCAt > 8:353431/1‑149 (MQ=255) gagaTCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTa < 5:15985/149‑1 (MQ=255) aTCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCa < 4:350173/110‑1 (MQ=255) aTCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCa > 3:350173/1‑110 (MQ=255) cgacgaACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACAttt > 5:396651/1‑149 (MQ=255) gacgaACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTc < 3:329636/149‑1 (MQ=255) acgaACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCa < 3:197996/149‑1 (MQ=255) cgaACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCAc < 7:380855/149‑1 (MQ=255) gtggAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGCTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAgg > 4:89602/1‑148 (MQ=255) aaaGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTc > 7:387251/1‑149 (MQ=255) ccGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCGAGCACCGCGCAGGCGGTGCTTGATct < 2:390520/109‑2 (MQ=38) ccGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCa > 3:379909/1‑148 (MQ=255) ccGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACAATTCACGTCAGGCACCTTCCGCa > 5:188352/1‑148 (MQ=255) cAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGc < 6:35510/149‑1 (MQ=255) gCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCaaaat > 4:271070/1‑145 (MQ=255) gacctgacctATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCttt < 4:379909/149‑1 (MQ=255) ctgacctATGAGGCTGTTCGGTAACAGCGGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCttt > 1:46669/1‑146 (MQ=255) ctgacctATGAGGCTGTTCGGTAACAGCGGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCttt < 2:46669/146‑1 (MQ=255) gaactATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTATCACCACCTGTGAGATCAAGCACCGCATGCGCGGTGCACGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATATTCCTTTGTGa > 6:270401/4‑148 (MQ=255) acctATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCAAAATGCTTtgcgg < 3:271070/147‑4 (MQ=255) tATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCCAAGCATTTTGCTTTGTGAAATg > 5:319912/1‑148 (MQ=255) aTGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCGCCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGaa > 3:30911/1‑149 (MQ=255) tGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTa > 2:106601/1‑149 (MQ=255) tGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTa > 5:183481/1‑149 (MQ=255) cGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACttt > 2:437187/1‑149 (MQ=255) gTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTc > 8:89865/1‑149 (MQ=255) gTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTc > 4:199550/1‑149 (MQ=255) aCAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTgg < 8:387251/149‑1 (MQ=255) agcagAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTgg < 1:109670/147‑1 (MQ=255) agcagAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTgg > 2:109670/1‑147 (MQ=255) ccTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGAACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTc < 4:35726/149‑1 (MQ=255) cTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTcc < 5:400229/149‑1 (MQ=255) gTTAGCTGTTCTCAGCACCTGTGAGATCAAGCGCCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGt < 4:30911/149‑1 (MQ=255) ttCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGt > 3:162102/1‑149 (MQ=255) tctcAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTc > 5:105270/1‑149 (MQ=255) tcAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTcgc < 3:345722/149‑1 (MQ=255) tcAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTcgc > 2:367531/1‑149 (MQ=255) aGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGa > 3:275719/1‑149 (MQ=255) aCCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGAtt > 8:284208/1‑148 (MQ=255) gaTCGAGCACCGCGCAGGCGGTGCTTGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATg > 1:218696/1‑148 (MQ=255) gaTCGAGCACCGCGCAGGCGGTGCTTGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATg > 3:49874/1‑148 (MQ=255) gaTCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGa < 8:25595/149‑1 (MQ=255) tCAAGCACCGCCTGCGCTGTGCTCTATCGTTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTTTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGaaa < 3:89602/149‑1 (MQ=255) tCAACCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGaaa < 7:362240/149‑1 (MQ=255) cAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGAAAg > 1:341352/1‑149 (MQ=255) aaGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCt < 7:412246/104‑1 (MQ=255) aaGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCt > 8:412246/1‑104 (MQ=255) aaGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGAAAGt > 3:417524/1‑149 (MQ=255) | ACCATGATCATCGCGCCACCGCTGGTGATCAGTATCGAAGAGATCGACGAACTGGTGGAGAAAGCCCGCAAGTGCCTGGACCTGACCTATGAGGCTGTTCGGTAACAGCAGAGGGCCTGGTTAGCTGTTCTCAGCACCTGTGAGATCAAGCACCGCCTGCGCGGTGCTCGATCGGTGCCGCCATCGTACATTTCACGTCAGGCACCTTCCGCAAAGCATTTTGCTTTGTGAAATGAATCCTGTACTTTTCTGGGCATCCATTTCCCGTCTGAAAGTCGCGATTCAACCATGAAAGT > NC_002947/2486663‑2486958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |