Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,502,985	A→T	7.7%	S94C (AGC→TGC)	speB →	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,502,985	0	A	T	7.7%	194.6 / 4.9	65	S94C (AGC→TGC)	speB	agmatinase
Reads supporting (aligned to +/- strand):  ref base A (34/26);  new base T (3/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCCACCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGC  >  NC_002947/2502838‑2503133                                                                                                                                                    |                                                                                                                                                     gCCACCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAg                                                                                                                                                     <  2:428614/149‑1 (MQ=255)    accaGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCgg                                                                                                                                                  <  6:182206/149‑1 (MQ=255)     ccaGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGc                                                                                                                                                 >  8:289118/1‑149 (MQ=255)               ccccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGt                                                                                                                                       <  3:249135/149‑1 (MQ=255)                 ccGGCGCGCGCCTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                                     >  4:409921/1‑149 (MQ=255)                  cGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCgg                                                                                                                                    <  1:427534/149‑1 (MQ=255)                       cgcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaaggga                                                                                                                               <  5:39140/149‑7 (MQ=255)                          gcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCACTTCGACAGCCGCACGCCGCACTCTGTACCTGa                                                                                                                             >  5:117994/1‑148 (MQ=255)                            ttCGGCCCGCGGGCGATCCGTACTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAg                                                                                                                          >  5:184583/1‑149 (MQ=255)                              cGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGc                                                                                                                         >  2:37625/1‑148 (MQ=255)                                   cgcgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCCGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAg                                                                                                                   <  4:245581/149‑1 (MQ=255)                                    gcgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAgg                                                                                                                  <  7:5454/149‑1 (MQ=255)                                     cgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGc                                                                                                                 <  6:79833/149‑1 (MQ=255)                                                tgctgcCTCGGTCCAGCAGGCCTGGGGCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAg                                                                                                       >  7:117281/1‑148 (MQ=255)                                                  ctgcATCGGTACAGCAGGCCTGTGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGca                                                                                                     <  6:117994/148‑1 (MQ=255)                                                        cGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTg                                                                                              >  5:17463/1‑149 (MQ=255)                                                         ggTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGc                                                                                             <  6:184583/149‑1 (MQ=255)                                                            ccAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAgg                                                                                          >  8:309041/1‑149 (MQ=255)                                                             cagcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGc                                                                                         >  2:123178/1‑149 (MQ=255)                                                              agcagGCCTGGGCCCGCCAATGGCCGTGGGCGTTCGACCCGTTCGACAACCTGGCGGTCATCGACTATGGAGACTGTCCCTTCGAAAGCGGCACGCCGCAGTCGGTACCGGACAGAATCGAGGCACAAGCCTAGCACATTCTTAAGGCg                                                                                        >  6:227390/1‑149 (MQ=255)                                                               gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                       <  1:123178/149‑1 (MQ=255)                                                               gcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                       <  4:74327/149‑1 (MQ=255)                                                                     cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTgcg                                                                                 >  6:250427/1‑149 (MQ=255)                                                                     cTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTgcg                                                                                 >  6:314297/1‑149 (MQ=255)                                                                        ggCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCTGGTTGCGCCa                                                                              <  5:66028/149‑1 (MQ=255)                                                                             gCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                       >  6:330666/1‑135 (MQ=255)                                                                             gCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                       <  5:330666/135‑1 (MQ=255)                                                                             gCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCt                                                                          >  8:410728/1‑148 (MQ=255)                                                                                aCTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGAATGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAcc                                                                      <  4:347053/149‑1 (MQ=255)                                                                                 cTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAccc                                                                     >  7:155447/1‑149 (MQ=255)                                                                                  tGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCt                                                                    >  8:100073/1‑149 (MQ=255)                                                                                  tGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCt                                                                    >  5:403008/1‑149 (MQ=255)                                                                                    gCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtg                                                                  <  7:289118/149‑1 (MQ=255)                                                                                     ccGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                                 <  8:117281/149‑1 (MQ=255)                                                                                         gggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                                 <  4:153521/145‑1 (MQ=255)                                                                                         gggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                                 >  3:153521/1‑145 (MQ=255)                                                                                         gggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGc                                                             <  6:403008/149‑1 (MQ=255)                                                                                             gttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                          >  6:52642/1‑148 (MQ=255)                                                                                             gttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGAc                                                          >  1:249342/1‑148 (MQ=255)                                                                                              ttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCa                                                        >  3:27950/1‑149 (MQ=255)                                                                                                  acccgttcgaccACCTGGCGGTCATCGACTATGGAGACTGTCCCTTCGACAGCTGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGTCGGGTTTCGCCATGCTGACCCTTGGTGGCGACCACTtc                                                    <  5:227390/149‑1 (MQ=255)                                                                                                      gttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGTACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGa                                                           <  5:290688/138‑1 (MQ=255)                                                                                                      gttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGTACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGa                                                           >  6:290688/1‑138 (MQ=255)                                                                                                       gatgaccGCCAGGTGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAg                                               >  7:383090/4‑149 (MQ=255)                                                                                                          gaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTa                                            >  3:299323/1‑149 (MQ=255)                                                                                                             caccTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGAGCCATGCTGACCCTAGGTGGCGACAACTTCATCAGCTAcac                                         >  6:403928/1‑147 (MQ=255)                                                                                                                            gACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTc                           <  7:410728/148‑1 (MQ=255)                                                                                                                            gACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCa                          >  7:130107/1‑149 (MQ=255)                                                                                                                             aCTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAc                         >  8:179384/1‑149 (MQ=255)                                                                                                                                   gCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcgcccgc                   <  5:413237/149‑1 (MQ=255)                                                                                                                                      aCTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCcgccgc                <  4:299323/149‑1 (MQ=255)                                                                                                                                       cTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCAACCCGCTGCTCAAGGCTCACGCCCgccgcc               <  1:37625/149‑1 (MQ=255)                                                                                                                                        tGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa              >  4:271128/1‑149 (MQ=255)                                                                                                                                        tGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCa              >  5:209467/1‑149 (MQ=255)                                                                                                                                          tCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg            >  7:280469/1‑149 (MQ=255)                                                                                                                                            ccTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGc          <  5:52642/149‑1 (MQ=255)                                                                                                                                             cTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCACCGCTCACGCCCGCCGCCATGGcc         >  3:104873/1‑149 (MQ=255)                                                                                                                                              ttCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAc                                                                       <  3:59648/86‑1 (MQ=255)                                                                                                                                              ttCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAc                                                                       >  4:59648/1‑86 (MQ=255)                                                                                                                                                ccacacCGGCACGCCGCCGCCGGCACCGCACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGccc        <  3:319065/145‑1 (MQ=255)                                                                                                                                                cGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg            <  3:267124/143‑1 (MQ=255)                                                                                                                                                cGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg            >  4:267124/1‑143 (MQ=255)                                                                                                                                                cGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGc      >  4:115320/1‑149 (MQ=255)                                                                                                                                                cGACAGCGGCACGCCGCAGTCGGTACCGGACAGGATCGAGGCCCATGCCGAGCAGAGTCTGGAGGCGCGTTGCGGCGGGCTGGCCCGGGGTGGCGAGCGCGTCGTGGGCGGGCCGCTGCGGCCGGCTCAGGCCGGCCGCCAGGGcgcgg      >  8:313415/1‑148 (MQ=255)                                                                                                                                                 gACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCt     >  1:352174/1‑149 (MQ=255)                                                                                                                                                  aCAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTg    >  3:20587/1‑149 (MQ=255)                                                                                                                                                    aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgc  >  1:426811/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCCACCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGC  >  NC_002947/2502838‑2503133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑