Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,649,769 C→T 7.8% P229L (CCG→CTG)  PP_2320 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,649,7690CT 7.8% 211.6 / 6.2 77P229L (CCG→CTG) PP_2320hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/40);  new base T (3/3);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

GTTCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTGCTCAAGCGCG  >  NC_002947/2649623‑2649912
                                                                                                                                                  |                                                                                                                                               
gTTCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCgg                                                                                                                                               >  7:363243/1‑149 (MQ=255)
gTTCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCgg                                                                                                                                               >  5:263296/1‑149 (MQ=255)
gTTCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCgg                                                                                                                                               >  3:49136/1‑149 (MQ=255)
 ttCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGt                                                                                                                                              <  6:37026/149‑1 (MQ=255)
   cAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAATAAGTTCGGCATTGTCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACTTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTg                                                                                                                                             <  5:225018/148‑1 (MQ=255)
     aGTTCACCTTGGGCGTACCGGGCTTCCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCCCCAGTCACGGTTCCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTgcgc                                                                                                                                          >  5:209193/1‑149 (MQ=255)
      gTTCACCTAGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGca                                                                                                                                         >  1:98531/1‑149 (MQ=255)
       ttCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGATGATGGTGCCGGTGGGGACTCCGGTGCGcat                                                                                                                                        >  2:34942/1‑149 (MQ=255)
         cACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGcatc                                                                                                                                       <  8:395394/148‑1 (MQ=255)
          aCCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGcatcat                                                                                                                                     >  4:168047/1‑149 (MQ=255)
          aCCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGcatca                                                                                                                                      >  7:365935/1‑148 (MQ=255)
             ttGAGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCaa                                                                                                                                  >  6:316934/1‑149 (MQ=255)
               gggCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAAc                                                                                                                                 <  5:188645/148‑1 (MQ=255)
                 gCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAg                                                                                                                              >  1:371436/1‑149 (MQ=255)
                 gCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAg                                                                                                                              >  1:377134/1‑149 (MQ=255)
                 gCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAg                                                                                                                              >  1:179804/1‑149 (MQ=255)
                      ccGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTa                                                                                                                         <  5:83458/149‑1 (MQ=255)
                       cGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTa                                                                                                                         >  4:233279/1‑148 (MQ=255)
                              ccTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGAGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCg                                                                                                                 >  5:373370/1‑149 (MQ=255)
                                  aTCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCGCCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTAt                                                                                                             >  7:384636/1‑149 (MQ=255)
                                  aTCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGt                                                                                                               <  8:365935/147‑1 (MQ=255)
                                      aTGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAgc                                                                                                         >  6:202046/1‑149 (MQ=255)
                                        ggTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAgtgc                                                                                                       >  6:155264/1‑149 (MQ=255)
                                         gTTCGAACAAGAAGTTCGGCATTGGCATGGGCACGAGTGACGGTTGCTTCCGTATGTTCAAGAACAACGTTCTTGAACTGTCGAAGATGGTGCCGGTGTGGACTCCGGTGAGCATCATCAACGAGCCTTACAAGTTCGGTATCAgcgc                                                                                                       <  4:396480/148‑1 (MQ=255)
                                                  agaagTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTc                                                                                             >  3:144745/1‑149 (MQ=255)
                                                      gTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATc                                                                                         >  7:69378/1‑149 (MQ=255)
                                                       ttCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCt                                                                                        <  7:242466/149‑1 (MQ=255)
                                                           gCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGCTCGGTATCAGCGCTGGCAAGGTCTATCTTGAg                                                                                    <  2:220477/149‑1 (MQ=255)
                                                                ggCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGCGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGcac                                                                                <  6:188438/148‑1 (MQ=255)
                                                                 gCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGcaca                                                                               <  6:263296/148‑1 (MQ=255)
                                                                   aTGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCacacac                                                                            >  1:361446/1‑149 (MQ=255)
                                                                     gcgcACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGc                                                                          >  3:324251/1‑149 (MQ=255)
                                                                      cgcACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGc                                                                          <  8:363243/148‑1 (MQ=255)
                                                                      cgcACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGc                                                                          >  1:91551/1‑148 (MQ=255)
                                                                        cACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCAc                                                                       <  5:202046/149‑1 (MQ=255)
                                                                         aCCAGGCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACt                                                                      <  2:264560/149‑1 (MQ=255)
                                                                           cAGTCACGGTTGCTTCCGCATGTTCAACAACGACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATAATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTgg                                                                    <  2:84922/149‑1 (MQ=255)
                                                                           cAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTgg                                                                    <  2:179804/149‑1 (MQ=255)
                                                                           cAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTCCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTg                                                                     >  5:222158/1‑148 (MQ=255)
                                                                            aGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGa                                                                   <  2:98531/149‑1 (MQ=255)
                                                                                    ttGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAgg                                                           <  2:371436/149‑1 (MQ=255)
                                                                                    ttGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAgg                                                           <  2:91551/149‑1 (MQ=255)
                                                                                     tGCTTCCGAATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCATCGCTGGCAATGTCTATCTTGAGGCACACACGCCACTGGATGACCAgg                                                           <  2:377134/148‑1 (MQ=255)
                                                                                        ttCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTaa                                                       >  4:180580/1‑149 (MQ=255)
                                                                                           cGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAAcc                                                     <  2:143963/148‑1 (MQ=255)
                                                                                             cATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGAGCTGGCAAGGTCTATATTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGt                                                  >  8:69032/1‑149 (MQ=255)
                                                                                               tGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCg                                                <  5:316934/149‑1 (MQ=255)
                                                                                               tGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCg                                                >  8:302726/1‑149 (MQ=255)
                                                                                                 ttCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGAACACACGCCACTGGATGACCAGGGTAACCCGTCGGt                                              >  7:410503/1‑149 (MQ=255)
                                                                                                   caccaacaccGTGCTTGAACTGTCCAAGACGGTCCCGGTGGCGACTCCCGTGCGCATCAACAACGAGCCTTACCAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGtt                                             <  4:49136/145‑1 (MQ=255)
                                                                                                   caacaacaaCGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTg                                            >  2:210355/1‑149 (MQ=255)
                                                                                                     acaacaacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTc                                          <  1:370254/149‑1 (MQ=255)
                                                                                                      caacaacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTc                                          >  3:400357/1‑148 (MQ=255)
                                                                                                       aacaacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGa                                        >  4:3364/1‑149 (MQ=255)
                                                                                                       aacaacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGa                                        <  4:144745/149‑1 (MQ=255)
                                                                                                        acaacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGAc                                       <  3:233279/149‑1 (MQ=255)
                                                                                                         caacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGAc                                       >  4:306911/1‑148 (MQ=255)
                                                                                                         caacGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACa                                      <  3:180580/149‑1 (MQ=255)
                                                                                                           acGTGGTTGAACTGTCGAAGATGGTGCCTGTGGGGAGTCCGGTGCGCATCATGAACGAGCCTTACTAGTTCGGTATCAGCGCTGGCAAGTTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACaaa                                    <  6:209193/149‑1 (MQ=255)
                                                                                                           acGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCTGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACaaa                                    <  2:361446/149‑1 (MQ=255)
                                                                                                                cTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAacaca                                <  4:131213/148‑1 (MQ=255)
                                                                                                                   gAACTGTCGAAGATGTTGCCGGTGGTGACTCCGGTGCGCATCATCAACGACCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCg                             <  1:34942/148‑1 (MQ=255)
                                                                                                                   gAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCg                             <  6:331500/148‑1 (MQ=255)
                                                                                                                      cTGTCGAAGATGGTGGCGGTGGGGACTCCTGTGCGCATAATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGt                         <  3:18008/149‑1 (MQ=255)
                                                                                                                       tGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAacac                                 <  1:361757/140‑1 (MQ=255)
                                                                                                                       tGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAacac                                 >  2:361757/1‑140 (MQ=255)
                                                                                                                           gAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCa                    <  4:324251/149‑1 (MQ=255)
                                                                                                                           gAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCa                    <  7:302726/149‑1 (MQ=255)
                                                                                                                            aaGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCaa                   <  8:69378/149‑1 (MQ=255)
                                                                                                                            aaGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCATTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGAACAGGGTAACCAGTCGGTTGTCGACAAACACACCGCAGTTATCaa                   >  4:410813/1‑149 (MQ=255)
                                                                                                                                tGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGTGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGcc               <  5:155264/149‑1 (MQ=255)
                                                                                                                                   tGCCGGTGGTGACTCCGGTGCGCATCATCAACGAGCATTACAAGTTCTGTATCAGCGCTGGCAAGGTCTCTCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGGCGACAAACACACCGCAGTTATCAATGCCTTg            <  5:403303/149‑1 (MQ=255)
                                                                                                                                           gggACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTGCTCAAgc     <  4:400357/148‑1 (MQ=255)
                                                                                                                                            ggACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTg            >  2:62541/1‑140 (MQ=255)
                                                                                                                                            ggACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTg            <  1:62541/140‑1 (MQ=255)
                                                                                                                                             gACTCCGTTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTGCTCAAGcgcg  <  8:384636/149‑1 (MQ=255)
                                                                                                                                             gACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTGCTCAAGcgcg  <  1:210355/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTTCAAGTTCACCTTGGGCGTACCGGGCTACCTGATCCATGGTTCGAACAAGAAGTTCGGCATTGGCATGCGCACCAGTCACGGTTGCTTCCGCATGTTCAACAACAACGTGCTTGAACTGTCGAAGATGGTGCCGGTGGGGACTCCGGTGCGCATCATCAACGAGCCTTACAAGTTCGGTATCAGCGCTGGCAAGGTCTATCTTGAGGCACACACGCCACTGGATGACCAGGGTAACCCGTCGGTTGTCGACAAACACACCGCAGTTATCAATGCCTTGCTCAAGCGCG  >  NC_002947/2649623‑2649912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑