Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 233,832 A→T 7.6% intergenic (+20/+43) PP_0180 → / ← PP_0181 cytochrome c family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947233,8320AT 7.6% 167.6 / 5.6 66intergenic (+20/+43)PP_0180/PP_0181cytochrome c family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/33);  new base T (2/3);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

GTTCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGC  >  NC_002947/233686‑233980
                                                                                                                                                  |                                                                                                                                                    
gTTCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCCGTCCcaca                                                                                                                                                    >  3:91545/1‑149 (MQ=255)
   cGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAgg                                                                                                                                                  <  7:144120/148‑1 (MQ=255)
         gctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACcgcg                                                                                                                                           <  7:384775/149‑1 (MQ=255)
          ctggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCTGTGGGACCgcgc                                                                                                                                          <  7:163968/149‑1 (MQ=255)
           tggGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTACGCGGTCCCTGTGGGACCGCGCa                                                                                                                                         <  8:361594/149‑1 (MQ=255)
             gggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGGGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCaa                                                                                                                                        <  4:330478/148‑1 (MQ=255)
                   cATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtctc                                                                                                                                  <  6:175988/148‑1 (MQ=255)
                      gCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCca                                                                                                                                                      >  6:353337/1‑125 (MQ=255)
                      gCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCca                                                                                                                                                      <  5:353337/125‑1 (MQ=255)
                       ccGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTGCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCAGAGGGACCGCGCAAGGTCTCAGAt                                                                                                                              <  6:12493/148‑1 (MQ=255)
                         gATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCtt                                                                                                                           <  4:27268/149‑1 (MQ=255)
                           tGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgc                                                                                                                         <  8:284817/149‑1 (MQ=255)
                             ccTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgcg                                                                                                                        <  4:91545/148‑1 (MQ=255)
                             ccTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgcg                                                                                                                        >  1:360943/1‑148 (MQ=255)
                              cTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgcgc                                                                                                                       >  4:379308/1‑148 (MQ=255)
                               tACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgc                                                                                                                         <  4:364470/145‑1 (MQ=255)
                               tACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTgc                                                                                                                         >  3:364470/1‑145 (MQ=255)
                                 cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTg                                                                                                                          <  6:187459/142‑1 (MQ=255)
                                 cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTg                                                                                                                          >  5:187459/1‑142 (MQ=255)
                                       gTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGc                                                                                                                   >  5:430174/1‑143 (MQ=255)
                                       gTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGc                                                                                                                   <  6:430174/143‑1 (MQ=255)
                                        tCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATg                                                                                                             <  1:110465/148‑1 (MQ=255)
                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCCCACGGACCCCCCAAGGTCTCAGATCTTTCGCAAGCCAGagcat                                                                                                           >  6:27369/1‑144 (MQ=255)
                                          ggCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGagcatc                                                                                                          <  5:27369/149‑6 (MQ=255)
                                           gcgcAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATATTgcg                                                                                                                        >  8:89477/1‑134 (MQ=255)
                                           gcgcAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATATTgcg                                                                                                                        <  7:89477/134‑1 (MQ=255)
                                               aaGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAggct                                                                                                     <  8:311885/149‑1 (MQ=255)
                                               aaGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCCGTCCCACAGGGAACCCGCAAGGTCTCCGATATTGCGCAAGCCTGATGcaaacgct                                                                                                     >  4:248043/1‑142 (MQ=255)
                                                         cTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTTCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGgcg                                                                                           >  6:127108/1‑149 (MQ=255)
                                                         cTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCGGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGAGCATCAggcttgcgcaagat                                                                                           <  7:398253/149‑8 (MQ=255)
                                                             cTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCtt                                                                                       <  3:379308/149‑1 (MQ=255)
                                                             aaggCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCtt                                                                                       <  4:116257/147‑1 (MQ=255)
                                                              tGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTACCCCAGGGGCCGCGCAAGGTGTCAGATCTTTCGCAAGCCTGATGCTCAGGCTGGCTGTGgcggcgtg                                                                                      >  4:253720/1‑144 (MQ=255)
                                                                  cATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCACAGAACTTGCGCAAGCCTGATGCTAAGGCTGGCTGTGGCGCCTTGATaa                                                                                  >  6:418141/1‑149 (MQ=255)
                                                                      gtgtTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGtt                                                                              <  8:363894/149‑1 (MQ=255)
                                                                         ttCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTg                                                                           <  2:234273/149‑1 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGATAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTa                                                                         <  1:388699/148‑1 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAggctgg                                                                                                   >  5:366421/1‑122 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAggctgg                                                                                                   <  6:366421/122‑1 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTa                                                                         <  3:107143/148‑1 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTa                                                                         <  4:153185/148‑1 (MQ=255)
                                                                            cTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAg                                                                        <  7:144175/149‑1 (MQ=255)
                                                                                   gCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTGCCACAGGGCCCGCGCAAGGTCTCAGATCTTGCGCAAGCCGGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTGTGTAGCAAtttg                                                                 >  3:242766/1‑147 (MQ=255)
                                                                                        tCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCCCGCAAGGTCTCAGATCTTGCGCAATCCAGAAGCTCAGGCTGGCGGTGGCGCCTTGAGAATTTTGTGTAGGAATTGGAAACt                                                            >  2:290629/1‑149 (MQ=255)
                                                                                            ggctggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCCCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTc                                                         >  8:212865/1‑148 (MQ=255)
                                                                                                 gcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACg                                                     <  7:212865/147‑1 (MQ=255)
                                                                                                  ccGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACCGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCg                                                  >  3:142381/1‑149 (MQ=255)
                                                                                                     aaaaGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCa                                               <  2:181569/149‑1 (MQ=255)
                                                                                                        aGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGa                                            <  2:138156/149‑1 (MQ=255)
                                                                                                                   gcGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTg                                                                           <  1:317092/107‑1 (MQ=255)
                                                                                                                   gcGGCCAACTGACTGGAGCCTGCGCGGTACCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCAGTTGAGCCATGAGAATGTTATg                                                                           >  2:317092/1‑107 (MQ=255)
                                                                                                                      gCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGGGCAAGGGCTCAGATCTTGCGCAAGCCTGATGCTCAGGCGGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAg                              <  1:290629/149‑1 (MQ=255)
                                                                                                                      gCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAg                              <  3:113188/149‑1 (MQ=255)
                                                                                                                           ctgactgGAGCCTGCGCGGTTCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgc                         <  5:127108/149‑1 (MQ=255)
                                                                                                                              actgCAGCCTGCGCGGTCCCACAGGGACCGCGCAATGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgcc                        <  8:317183/147‑1 (MQ=255)
                                                                                                                                    gCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGGTGTTGCGCCTTGTGAATGTTCTGTAGCAATTGGACACTTTAAAAGGCGTCATGACACCGGCTGGTCAGGTATCCGCCATCCg                >  4:417271/1‑149 (MQ=255)
                                                                                                                                    gCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACACAGGCTGGTCAGGTAGCCGCCATCCg                >  8:282521/1‑149 (MQ=255)
                                                                                                                                     ccTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCCGGCTGGTCAGGTAGCCGCCATCCgg               >  1:393220/1‑149 (MQ=255)
                                                                                                                                      ttGCGCGGTCCCTGTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGc              >  7:342477/2‑149 (MQ=255)
                                                                                                                                          gcgGTCCCTGTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGt                                 <  6:412666/126‑1 (MQ=255)
                                                                                                                                          gcgGTCCCTGTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGt                                 >  5:412666/1‑126 (MQ=255)
                                                                                                                                          gcgGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATACTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGAt          >  1:100981/1‑149 (MQ=255)
                                                                                                                                            ggTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCg        >  1:243676/1‑149 (MQ=255)
                                                                                                                                              tCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt      >  4:138125/1‑149 (MQ=255)
                                                                                                                                                ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt      >  7:294293/1‑147 (MQ=255)
                                                                                                                                                ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt      >  1:410376/1‑147 (MQ=255)
                                                                                                                                                ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt      >  6:274791/1‑147 (MQ=255)
                                                                                                                                                ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGt      >  6:18337/1‑147 (MQ=255)
                                                                                                                                                ccACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCa    >  6:445371/1‑149 (MQ=255)
                                                                                                                                                 cacaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAg   >  3:4059/1‑149 (MQ=255)
                                                                                                                                                  acaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAg   >  6:310391/1‑148 (MQ=255)
                                                                                                                                                  acaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGc  >  7:185854/1‑149 (MQ=255)
                                                                                                                                                  acaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGc  >  6:220841/1‑149 (MQ=255)
                                                                                                                                                  acaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGc  >  4:167702/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTCGACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGC  >  NC_002947/233686‑233980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑