BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,670,775	A→T	7.5%	S515T (TCC→ACC)	acnB ←	aconitate hydratase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,670,775	0	A	T	7.5%	293.3 / 5.7	100	S515T (TCC→ACC)	acnB	aconitate hydratase B
Reads supporting (aligned to +/- strand): ref base A (45/47); new base T (5/3); total (50/50)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CCTTCTGGATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATGAA  >  NC_002947/2670628‑2670922

ccTTCTGGATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGaa                                                                                                                                                      <  3:253538/149‑1 (MQ=255)

ccTTCTGGATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGaa                                                                                                                                                      <  4:281243/149‑1 (MQ=255)

 cTTCTGGATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAAc                                                                                                                                                     <  6:4749/149‑1 (MQ=255)

       gATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGATcc‑ggccg                                                                                                                                              <  3:200345/149‑1 (MQ=255)

       gATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAAcc‑ggccg                                                                                                                                              >  7:113982/1‑149 (MQ=255)

       gATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAAcc‑ggccg                                                                                                                                              <  4:101607/149‑1 (MQ=255)

         tGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCAACCAGGCCGGAAcc‑ggccgg                                                                                                                                             >  1:87320/1‑148 (MQ=255)

            cGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AAc                                                                                                                                        >  1:349773/1‑149 (MQ=255)

                 taAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGAt                                                                                                                                   >  5:209492/1‑149 (MQ=255)

                  aaGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACga                                                                                                                                      >  7:370420/1‑145 (MQ=255)

                  aaGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACga                                                                                                                                      <  8:370420/145‑1 (MQ=255)

                      gATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGa                                                                                                                              <  8:113982/149‑1 (MQ=255)

                        tGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGTATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATc                                                                                                                            <  5:313952/149‑1 (MQ=255)

                            aTGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCggg                                                                                                                         >  4:356820/1‑148 (MQ=255)

                            aTGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCggg                                                                                                                         <  3:356820/148‑1 (MQ=255)

                            aTGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCggg                                                                                                                         >  1:190322/1‑148 (MQ=255)

                              gCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCCGGCCGGTTCC‑GGCCtggtggc                                                                                                                                        <  1:312604/132‑5 (MQ=255)

                              gCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCCGGCCGGTTCC‑GGCCtggtggc                                                                                                                                        >  2:312604/1‑128 (MQ=255)

                              gCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGACCC‑GGCCGGG‑AACGAGATGCCGATCGGGGAg                                                                                                                      >  3:44109/1‑149 (MQ=255)

                               cACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGc                                                                                                                     >  6:323241/1‑149 (MQ=255)

                                aCCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCg                                                                                                                    >  7:114611/1‑149 (MQ=255)

                                 ccAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCgg                                                                                                                   <  1:432786/149‑1 (MQ=255)

                                 ccAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCgg                                                                                                                   >  5:285335/1‑149 (MQ=255)

                                     gTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGgtg                                                                                                                <  8:114611/148‑1 (MQ=255)

                                        aCGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGT‑AACGAGATGCCGATCTGGAAGCGGGTGTGc                                                                                                             <  2:87320/148‑1 (MQ=255)

                                        aCGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGc                                                                                                             >  4:4904/1‑148 (MQ=255)

                                          gCAAGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAg                                                                                                          >  1:421913/1‑149 (MQ=255)

                                                 gATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCAc                                                                                                   <  6:415228/149‑1 (MQ=255)

                                                   tGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCg                                                                                                 >  5:96719/1‑149 (MQ=255)

                                                    gCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCgg                                                                                                >  2:165743/1‑149 (MQ=255)

                                                      cTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTa                                                                                              <  6:55526/149‑1 (MQ=255)

                                                      cTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTa                                                                                              >  3:8116/1‑149 (MQ=255)

                                                      cTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACCCCGGTGGCGGCAGCGAAGGCCCCCAGGCCGGAAAC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTa                                                                                              >  8:278971/1‑149 (MQ=255)

                                                        ggCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAcc                                                                                            >  6:202402/1‑149 (MQ=255)

                                                         gCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGGCAACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAc                                                                                             <  1:95704/148‑1 (MQ=255)

                                                         gCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAcc                                                                                            >  4:53433/1‑148 (MQ=255)

                                                         gCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAcc                                                                                            >  3:386914/1‑148 (MQ=255)

                                                         gCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAc                                                                                             <  3:28208/147‑1 (MQ=255)

                                                         gCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTAc                                                                                             <  8:34149/147‑1 (MQ=255)

                                                          cTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTa                                                                                          >  6:69219/1‑149 (MQ=255)

                                                           tGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTAc                                                                                         >  1:56486/1‑149 (MQ=255)

                                                           tGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTAc                                                                                         >  7:406176/1‑149 (MQ=255)

                                                             cAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTAc                                                                                         <  1:394523/147‑1 (MQ=255)

                                                             cAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACg                                                                                        <  6:209492/148‑1 (MQ=255)

                                                             cAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACg                                                                                        <  8:151933/148‑1 (MQ=255)

                                                               gTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGgtg                                                                                     >  2:434580/1‑149 (MQ=255)

                                                                   gCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGTCGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCa                                                                                  <  6:385612/148‑1 (MQ=255)

                                                                   gCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCa                                                                                  <  6:96719/148‑1 (MQ=255)

                                                                   gCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCa                                                                                  <  5:69219/148‑1 (MQ=255)

                                                                    cccTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAgg                                                                                <  5:392901/149‑1 (MQ=255)

                                                                      cTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCa                                                                              >  4:344770/1‑149 (MQ=255)

                                                                         gAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCAt                                                                             >  2:96124/1‑147 (MQ=255)

                                                                         gAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATc                                                                            <  2:421913/148‑1 (MQ=255)

                                                                          aaGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCa                                                                           <  2:56486/148‑1 (MQ=255)

                                                                          aaGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAg                                                                          <  4:191530/149‑1 (MQ=255)

                                                                           aGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGc                                                                         <  3:344770/149‑1 (MQ=255)

                                                                            gcgcACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCa                                                                        >  8:182255/1‑149 (MQ=255)

                                                                                 ccAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCgg                                                                   <  1:434580/149‑1 (MQ=255)

                                                                                 ccAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGAACCTACGGTGTCAGGCATCAGCATGCgg                                                                   <  1:244909/149‑1 (MQ=255)

                                                                                      aTCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTcag                                                              >  1:100012/1‑149 (MQ=255)

                                                                                       tCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTcagc                                                             <  5:202402/149‑1 (MQ=255)

                                                                                          aTTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGa                                                                                                           <  2:303663/100‑1 (MQ=255)

                                                                                          aTTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGa                                                                                                           >  1:303663/1‑100 (MQ=255)

                                                                                          aTTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTcagccag                                                          >  5:194414/1‑149 (MQ=255)

                                                                                           ttCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTcagccagc                                                         >  6:425619/1‑149 (MQ=255)

                                                                                                gCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCtgtg                                                     <  5:323241/148‑1 (MQ=255)

                                                                                                 cATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGga                                                   <  1:165743/149‑1 (MQ=255)

                                                                                                   tGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACCGGGCCGGG‑AACGCCATGCCGATCGGGAAGCGGGTGTCCGAGGCCCCACCGGTACGAACGGTGTCAGGCATCAGCATGCGGTTCAGCCTGCGGTGgat                                                  >  1:331117/1‑149 (MQ=255)

                                                                                                   tGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGgatg                                                 <  3:53433/149‑1 (MQ=255)

                                                                                                      ccAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCgg                                                                   >  4:216293/1‑128 (MQ=255)

                                                                                                      ccAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGatgatg                                              >  2:144633/1‑149 (MQ=255)

                                                                                                        aGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGcc                                            <  2:190322/149‑1 (MQ=255)

                                                                                                          tGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGt                                          >  8:379595/1‑149 (MQ=255)

                                                                                                          tGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGt                                          <  2:349773/149‑1 (MQ=255)

                                                                                                            gCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCg                                        >  2:190008/1‑149 (MQ=255)

                                                                                                             cATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGc                                       <  6:208885/149‑1 (MQ=255)

                                                                                                                  cGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCggg                                  >  1:247095/1‑149 (MQ=255)

                                                                                                                  cGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCggg                                  <  3:115362/149‑1 (MQ=255)

                                                                                                                     cGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGgcg                                >  4:309717/1‑148 (MQ=255)

                                                                                                                           cggcAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGa                          >  3:144241/1‑148 (MQ=255)

                                                                                                                               aGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCtgt                                                      <  1:118474/116‑1 (MQ=255)

                                                                                                                               aGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCtgt                                                      >  2:118474/1‑116 (MQ=255)

                                                                                                                               aGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACAcgc                     >  4:228536/1‑149 (MQ=255)

                                                                                                                                  gAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGga                                                   <  5:274447/116‑1 (MQ=255)

                                                                                                                                  gAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGga                                                   >  6:274447/1‑116 (MQ=255)

                                                                                                                                       gttcccggCCGGTTCC‑GGCCTGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACgg             >  7:189141/7‑149 (MQ=255)

                                                                                                                                        ttcccggCCGGTTCC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACggg            >  3:110808/6‑149 (MQ=255)

                                                                                                                                        ttcccggCCGGTTCC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACggg            >  1:219543/6‑149 (MQ=255)

                                                                                                                                        ttcccggCCGGTTCC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCCGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGCCACGCCGCCACggg            >  7:28833/6‑149 (MQ=255)

                                                                                                                                         accaGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGt           >  3:239900/1‑149 (MQ=255)

                                                                                                                                         accaGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGt           >  4:293477/1‑149 (MQ=255)

                                                                                                                                           ccggCCGGTTCC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGc         <  2:219543/147‑1 (MQ=255)

                                                                                                                                             ggCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCgg       <  7:182255/149‑1 (MQ=255)

                                                                                                                                              gCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGa      <  1:190008/149‑1 (MQ=255)

                                                                                                                                               ccGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGAt     >  4:220509/1‑149 (MQ=255)

                                                                                                                                               ccGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGAt     <  1:145595/149‑1 (MQ=255)

                                                                                                                                                cGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATg    <  3:293477/149‑1 (MQ=255)

                                                                                                                                                 ggAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATGa   <  3:4904/149‑1 (MQ=255)

                                                                                                                                                  gAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATGaa  <  8:145754/149‑1 (MQ=255)

                                                                                                                                                  gAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATGaa  <  4:404005/149‑1 (MQ=255)

CCTTCTGGATGGCGTAGTAAGGGATGGCATGCACCAGGTCACGCAGGGTGATGCCTGGCTGCAGTTTGCCCTTGAAGCGCACCAGGATCGATTCTGGCATGTCCAGTGGCATGACGCCGGTGGCGGCAGCGAAGGCCACCAGGCCGGAACC‑GGCCGGG‑AACGAGATGCCGATCGGGAAGCGGGTGTGCGAGTCGCCACCGGTACCTACGGTGTCAGGCATCAGCATGCGGTTCAGCCAGCTGTGGATGATGCCGTCGCCCGGGCGCAGCGACACGCCGCCACGGGTGCGGATGAA  >  NC_002947/2670628‑2670922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`