Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,678,020 C→T 5.6% L121L (CTG→TTG)  PP_2348 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,678,0200CT 5.6% 268.1 / 3.2 88L121L (CTG→TTG) PP_2348sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (38/45);  new base T (3/2);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

CTATCAGCGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTG  >  NC_002947/2677872‑2678166
                                                                                                                                                    |                                                                                                                                                  
cTATCAGCGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAAc                                                                                                                                                    >  4:199919/1‑149 (MQ=255)
   tCAGCGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGGCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAActg                                                                                                                                                  <  4:316451/148‑1 (MQ=255)
      gCGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTgg                                                                                                                                              <  5:285385/149‑1 (MQ=255)
       cGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGc                                                                                                                                             >  6:74509/1‑149 (MQ=255)
        ggCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGcc                                                                                                                                            <  1:87619/149‑1 (MQ=255)
         gCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGccc                                                                                                                                           >  5:331757/1‑149 (MQ=255)
           cGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTg                                                                                                                                         <  8:212711/149‑1 (MQ=255)
               ttCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGt                                                                                                                                      <  5:6507/148‑1 (MQ=255)
                  cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                   >  1:204273/1‑148 (MQ=255)
                  cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCg                                                                                                                                  >  3:365369/1‑149 (MQ=255)
                  cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCg                                                                                                                                  <  5:270740/149‑1 (MQ=38)
                  cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGCTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                   <  4:211693/148‑1 (MQ=255)
                   ggTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGa                                                                                                                                 <  3:199919/149‑1 (MQ=255)
                     ttACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGAc                                                                                                                                >  3:3429/1‑148 (MQ=255)
                       actactTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTAc                                                                                                                             >  8:391814/1‑149 (MQ=255)
                           ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                         <  5:221239/149‑1 (MQ=255)
                           ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                         <  5:74509/149‑1 (MQ=255)
                            ttCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgcg                                                                                                                        >  6:10178/1‑149 (MQ=255)
                               cGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCt                                                                                                                     >  4:100159/1‑149 (MQ=255)
                                 cATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTgg                                                                                                                   >  1:414223/1‑149 (MQ=255)
                                      aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              >  4:13344/1‑149 (MQ=255)
                                      aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              >  5:45477/1‑149 (MQ=255)
                                      aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              <  5:10178/149‑1 (MQ=255)
                                      aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              <  6:331757/149‑1 (MQ=255)
                                      aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              >  8:75375/1‑149 (MQ=255)
                                       tttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                              >  2:222147/1‑148 (MQ=255)
                                        ttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCACGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGa                                                                                                            >  8:225935/1‑149 (MQ=255)
                                              cAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACAtctc                                                                                                      >  3:398010/1‑149 (MQ=255)
                                                gggTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCg                                                                                                     >  2:211257/1‑148 (MQ=255)
                                                   tACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCa                                                                                                 <  8:204503/149‑1 (MQ=255)
                                                      cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                              >  3:76312/1‑149 (MQ=255)
                                                      cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                              >  5:44603/1‑149 (MQ=255)
                                                         gAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTg                                                                                           <  3:314940/149‑1 (MQ=255)
                                                            ggctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                        <  4:35194/147‑1 (MQ=255)
                                                            gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCt                                                                                         >  2:309993/1‑148 (MQ=255)
                                                            gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                        >  6:34767/1‑149 (MQ=255)
                                                              ctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGTCATCTCGATCAGTGTTGCTCAg                                                                                      <  7:273495/149‑1 (MQ=255)
                                                                 gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTATCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                      <  3:310109/146‑1 (MQ=255)
                                                                 gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                            >  2:280123/1‑140 (MQ=255)
                                                                 gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                            <  1:280123/140‑1 (MQ=255)
                                                                 gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                      <  2:414223/146‑1 (MQ=255)
                                                                 gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGAc                                                                                   >  3:110189/1‑149 (MQ=255)
                                                                   tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAActgct                                                                                                                                                <  1:296946/86‑1 (MQ=255)
                                                                   tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAActgct                                                                                                                                                >  2:296946/1‑86 (MQ=255)
                                                                   tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTa                                                                                 >  7:371400/1‑149 (MQ=255)
                                                                      cTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTAtt                                                                               >  2:1809/1‑148 (MQ=255)
                                                                         tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCa                                                                             <  7:391814/147‑1 (MQ=255)
                                                                         tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCAc                                                                            <  8:9227/148‑1 (MQ=255)
                                                                           ggACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCg                                                                          <  3:100159/148‑1 (MQ=255)
                                                                              ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                      <  2:204273/149‑1 (MQ=255)
                                                                              ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                      <  4:365369/149‑1 (MQ=255)
                                                                                  gACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGa                                                                  <  2:136605/149‑1 (MQ=255)
                                                                                   aCATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGaa                                                                 <  1:382482/149‑1 (MQ=255)
                                                                                        cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                    <  3:259319/125‑1 (MQ=255)
                                                                                        cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                    >  4:259319/1‑125 (MQ=255)
                                                                                         ccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                           >  4:97895/1‑149 (MQ=255)
                                                                                         ccAAGCCCGCAGCGCCCGGGCCTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                           >  3:311530/1‑149 (MQ=255)
                                                                                          cAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTcc                                                          <  8:371400/149‑1 (MQ=255)
                                                                                               ccGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGc                                                     <  1:211257/149‑1 (MQ=255)
                                                                                                 gCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCtt                                                   <  4:76312/149‑1 (MQ=255)
                                                                                                   aGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgc                                                 <  4:110189/149‑1 (MQ=255)
                                                                                                    gcgcCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgca                                                <  3:97895/149‑1 (MQ=255)
                                                                                                         cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                           <  5:277718/149‑1 (MQ=255)
                                                                                                         cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                           <  6:45477/149‑1 (MQ=255)
                                                                                                                  cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                  <  4:311530/149‑1 (MQ=255)
                                                                                                                  cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                  <  5:34767/149‑1 (MQ=255)
                                                                                                                  cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTg                                   <  4:398010/148‑1 (MQ=255)
                                                                                                                        ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                            <  6:44603/149‑1 (MQ=255)
                                                                                                                        ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                            >  1:380645/1‑149 (MQ=255)
                                                                                                                        ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                            <  7:225935/149‑1 (MQ=255)
                                                                                                                          aTGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGc                          >  4:396882/1‑149 (MQ=255)
                                                                                                                                tCGGCCCGGAAGGCCAGCAACTGCCGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                    >  4:11584/1‑149 (MQ=255)
                                                                                                                                tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGc                                <  6:410171/137‑1 (MQ=38)
                                                                                                                                tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                    >  6:5560/1‑149 (MQ=38)
                                                                                                                                tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCAGGCGCTTGCAGCAGATCGGCCTGGGc                                >  5:410171/1‑137 (MQ=37)
                                                                                                                                gcGGCCCTGAAGGCCAGCTACTGCTGGCCCTGCGGGCCGACTAGCGGCGCCTGGGCCAGGACTTCTCGATCAGTGTTGCTCAGGCCTATTCACCGGTTCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                    <  8:400796/148‑1 (MQ=255)
                                                                                                                                  ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                   <  7:75375/148‑1 (MQ=255)
                                                                                                                                  ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGgc                  >  3:363566/1‑149 (MQ=255)
                                                                                                                                  ggCCCGGAAGGACAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                   <  4:100466/148‑1 (MQ=255)
                                                                                                                                   gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                     <  5:271655/145‑1 (MQ=255)
                                                                                                                                   gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                     >  6:271655/1‑145 (MQ=255)
                                                                                                                                    cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                        >  2:305565/1‑125 (MQ=255)
                                                                                                                                    cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                        <  1:305565/125‑1 (MQ=255)
                                                                                                                                         agggCCAGCAGTTGCTGGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATc           >  8:227546/3‑149 (MQ=255)
                                                                                                                                            gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                              <  1:411227/111‑1 (MQ=255)
                                                                                                                                            gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                              >  2:411227/1‑111 (MQ=255)
                                                                                                                                              cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt      >  5:35451/1‑149 (MQ=255)
                                                                                                                                              cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt      <  1:309993/149‑1 (MQ=255)
                                                                                                                                              cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt      >  1:299019/1‑149 (MQ=255)
                                                                                                                                                gCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTc    >  7:301875/1‑149 (MQ=255)
                                                                                                                                                  aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg  >  7:314469/1‑149 (MQ=255)
                                                                                                                                                  aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg  <  8:261421/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTATCAGCGGCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTG  >  NC_002947/2677872‑2678166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑