Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,678,029	C→T	7.8%	L124L (CTG→TTG) ‡	PP_2348 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,678,029	0	C	T	7.8%	259.2 / 8.3	90	L124L (CTG→TTG) ‡	PP_2348	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (38/45);  new base T (3/4);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGC  >  NC_002947/2677881‑2678176                                                                                                                                                     |                                                                                                                                                    gCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGccc                                                                                                                                                     >  5:331757/1‑149 (MQ=255)   cGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTg                                                                                                                                                   <  8:212711/149‑1 (MQ=255)       ttCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGt                                                                                                                                                <  5:6507/148‑1 (MQ=255)          cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                             >  1:204273/1‑148 (MQ=255)          cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCg                                                                                                                                            >  3:365369/1‑149 (MQ=255)          cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCg                                                                                                                                            <  5:270740/149‑1 (MQ=38)          cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGCTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                             <  4:211693/148‑1 (MQ=255)           ggTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGa                                                                                                                                           <  3:199919/149‑1 (MQ=255)             ttACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGAc                                                                                                                                          >  3:3429/1‑148 (MQ=255)               actactTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTAc                                                                                                                                       >  8:391814/1‑149 (MQ=255)                   ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                   <  5:74509/149‑1 (MQ=255)                   ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                   <  5:221239/149‑1 (MQ=255)                    ttCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgcg                                                                                                                                  >  6:10178/1‑149 (MQ=255)                       cGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCt                                                                                                                               >  4:100159/1‑149 (MQ=255)                         cATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTgg                                                                                                                             >  1:414223/1‑149 (MQ=255)                              aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        >  4:13344/1‑149 (MQ=255)                              aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        <  6:331757/149‑1 (MQ=255)                              aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        >  8:75375/1‑149 (MQ=255)                              aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        <  5:10178/149‑1 (MQ=255)                              aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        >  5:45477/1‑149 (MQ=255)                               tttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                        >  2:222147/1‑148 (MQ=255)                                ttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCACGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGa                                                                                                                      >  8:225935/1‑149 (MQ=255)                                      cAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACAtctc                                                                                                                >  3:398010/1‑149 (MQ=255)                                        gggTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCg                                                                                                               >  2:211257/1‑148 (MQ=255)                                           tACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCa                                                                                                           <  8:204503/149‑1 (MQ=255)                                              cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                        >  5:44603/1‑149 (MQ=255)                                              cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                        >  3:76312/1‑149 (MQ=255)                                                 gAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTg                                                                                                     <  3:314940/149‑1 (MQ=255)                                                    ggctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                  <  4:35194/147‑1 (MQ=255)                                                    gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCt                                                                                                   >  2:309993/1‑148 (MQ=255)                                                    gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                  >  6:34767/1‑149 (MQ=255)                                                      ctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGTCATCTCGATCAGTGTTGCTCAg                                                                                                <  7:273495/149‑1 (MQ=255)                                                         gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTATCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                <  3:310109/146‑1 (MQ=255)                                                         gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                      <  1:280123/140‑1 (MQ=255)                                                         gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                      >  2:280123/1‑140 (MQ=255)                                                         gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                <  2:414223/146‑1 (MQ=255)                                                         gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGAc                                                                                             >  3:110189/1‑149 (MQ=255)                                                           tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTa                                                                                           >  7:371400/1‑149 (MQ=255)                                                              cTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTAtt                                                                                         >  2:1809/1‑148 (MQ=255)                                                                 tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCa                                                                                       <  7:391814/147‑1 (MQ=255)                                                                 tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCAc                                                                                      <  8:9227/148‑1 (MQ=255)                                                                   ggACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCg                                                                                    <  3:100159/148‑1 (MQ=255)                                                                      ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                <  2:204273/149‑1 (MQ=255)                                                                      ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                <  4:365369/149‑1 (MQ=255)                                                                          gACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGa                                                                            <  2:136605/149‑1 (MQ=255)                                                                           aCATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGaa                                                                           <  1:382482/149‑1 (MQ=255)                                                                                cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                              >  4:259319/1‑125 (MQ=255)                                                                                cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                              <  3:259319/125‑1 (MQ=255)                                                                                 ccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                     >  4:97895/1‑149 (MQ=255)                                                                                 ccAAGCCCGCAGCGCCCGGGCCTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                     >  3:311530/1‑149 (MQ=255)                                                                                  cAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTcc                                                                    <  8:371400/149‑1 (MQ=255)                                                                                       ccGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGc                                                               <  1:211257/149‑1 (MQ=255)                                                                                         gCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCtt                                                             <  4:76312/149‑1 (MQ=255)                                                                                           aGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgc                                                           <  4:110189/149‑1 (MQ=255)                                                                                            gcgcCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgca                                                          <  3:97895/149‑1 (MQ=255)                                                                                                 cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                     <  5:277718/149‑1 (MQ=255)                                                                                                 cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                     <  6:45477/149‑1 (MQ=255)                                                                                                          cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                            <  5:34767/149‑1 (MQ=255)                                                                                                          cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                            <  4:311530/149‑1 (MQ=255)                                                                                                          cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTg                                             <  4:398010/148‑1 (MQ=255)                                                                                                                ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                      >  1:380645/1‑149 (MQ=255)                                                                                                                ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                      <  7:225935/149‑1 (MQ=255)                                                                                                                ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                      <  6:44603/149‑1 (MQ=255)                                                                                                                  aTGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGc                                    >  4:396882/1‑149 (MQ=255)                                                                                                                        tCGGCCCGGAAGGCCAGCAACTGCCGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                              >  4:11584/1‑149 (MQ=255)                                                                                                                        tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGc                                          <  6:410171/137‑1 (MQ=38)                                                                                                                        tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                              >  6:5560/1‑149 (MQ=38)                                                                                                                        tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCAGGCGCTTGCAGCAGATCGGCCTGGGc                                          >  5:410171/1‑137 (MQ=37)                                                                                                                        gcGGCCCTGAAGGCCAGCTACTGCTGGCCCTGCGGGCCGACTAGCGGCGCCTGGGCCAGGACTTCTCGATCAGTGTTGCTCAGGCCTATTCACCGGTTCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                              <  8:400796/148‑1 (MQ=255)                                                                                                                          ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                             <  7:75375/148‑1 (MQ=255)                                                                                                                          ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGgc                            >  3:363566/1‑149 (MQ=255)                                                                                                                          ggCCCGGAAGGACAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                             <  4:100466/148‑1 (MQ=255)                                                                                                                           gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                               >  6:271655/1‑145 (MQ=255)                                                                                                                           gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                               <  5:271655/145‑1 (MQ=255)                                                                                                                            cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                  >  2:305565/1‑125 (MQ=255)                                                                                                                            cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                  <  1:305565/125‑1 (MQ=255)                                                                                                                                 agggCCAGCAGTTGCTGGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATc                     >  8:227546/3‑149 (MQ=255)                                                                                                                                    gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                        <  1:411227/111‑1 (MQ=255)                                                                                                                                    gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                        >  2:411227/1‑111 (MQ=255)                                                                                                                                      cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                >  5:35451/1‑149 (MQ=255)                                                                                                                                      cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                >  1:299019/1‑149 (MQ=255)                                                                                                                                      cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                <  1:309993/149‑1 (MQ=255)                                                                                                                                        gCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTc              >  7:301875/1‑149 (MQ=255)                                                                                                                                          aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg            >  7:314469/1‑149 (MQ=255)                                                                                                                                          aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg            <  8:261421/149‑1 (MQ=255)                                                                                                                                           gttgctgGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTgg           <  7:227546/147‑1 (MQ=255)                                                                                                                                             tgctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg         >  4:210896/1‑149 (MQ=255)                                                                                                                                               ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                      >  8:369652/1‑118 (MQ=255)                                                                                                                                               ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                      <  7:369652/118‑1 (MQ=255)                                                                                                                                               ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg         <  4:3429/147‑1 (MQ=255)                                                                                                                                                tgGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtgctg      <  2:319088/149‑1 (MQ=255)                                                                                                                                                    ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGTGCTGATCCTGATTCTGGTGCTGCAgc  <  8:25632/149‑1 (MQ=255)                                                                                                                                                    ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCa    >  1:344274/1‑147 (MQ=255)                                                                                                                                                    ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgc  <  1:1809/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCCGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGC  >  NC_002947/2677881‑2678176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑