Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,678,031	G→C	5.4%	L124L (CTG→CTC) ‡	PP_2348 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,678,031	0	G	C	5.4%	306.1 / 6.1	93	L124L (CTG→CTC) ‡	PP_2348	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (41/47);  new base C (2/3);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

CGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGCG  >  NC_002947/2677883‑2678177                                                                                                                                                     |                                                                                                                                                   cGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTg                                                                                                                                                    <  8:212711/149‑1 (MQ=255)     ttCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGt                                                                                                                                                 <  5:6507/148‑1 (MQ=255)        cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                              >  1:204273/1‑148 (MQ=255)        cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCg                                                                                                                                             >  3:365369/1‑149 (MQ=255)        cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCg                                                                                                                                             <  5:270740/149‑1 (MQ=38)        cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGCTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                              <  4:211693/148‑1 (MQ=255)         ggTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGa                                                                                                                                            <  3:199919/149‑1 (MQ=255)           ttACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGAc                                                                                                                                           >  3:3429/1‑148 (MQ=255)             actactTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTAc                                                                                                                                        >  8:391814/1‑149 (MQ=255)                 ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                    <  5:74509/149‑1 (MQ=255)                 ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                    <  5:221239/149‑1 (MQ=255)                  ttCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgcg                                                                                                                                   >  6:10178/1‑149 (MQ=255)                     cGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCt                                                                                                                                >  4:100159/1‑149 (MQ=255)                       cATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTgg                                                                                                                              >  1:414223/1‑149 (MQ=255)                            aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         >  4:13344/1‑149 (MQ=255)                            aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         <  6:331757/149‑1 (MQ=255)                            aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         >  8:75375/1‑149 (MQ=255)                            aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         <  5:10178/149‑1 (MQ=255)                            aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         >  5:45477/1‑149 (MQ=255)                             tttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                         >  2:222147/1‑148 (MQ=255)                              ttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCACGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGa                                                                                                                       >  8:225935/1‑149 (MQ=255)                                    cAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACAtctc                                                                                                                 >  3:398010/1‑149 (MQ=255)                                      gggTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCg                                                                                                                >  2:211257/1‑148 (MQ=255)                                         tACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCa                                                                                                            <  8:204503/149‑1 (MQ=255)                                            cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                         >  3:76312/1‑149 (MQ=255)                                            cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                         >  5:44603/1‑149 (MQ=255)                                               gAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTg                                                                                                      <  3:314940/149‑1 (MQ=255)                                                  ggctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                   <  4:35194/147‑1 (MQ=255)                                                  gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCt                                                                                                    >  2:309993/1‑148 (MQ=255)                                                  gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                   >  6:34767/1‑149 (MQ=255)                                                    ctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGTCATCTCGATCAGTGTTGCTCAg                                                                                                 <  7:273495/149‑1 (MQ=255)                                                       gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTATCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                 <  3:310109/146‑1 (MQ=255)                                                       gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                       <  1:280123/140‑1 (MQ=255)                                                       gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                       >  2:280123/1‑140 (MQ=255)                                                       gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                 <  2:414223/146‑1 (MQ=255)                                                       gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGAc                                                                                              >  3:110189/1‑149 (MQ=255)                                                         tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTa                                                                                            >  7:371400/1‑149 (MQ=255)                                                            cTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTAtt                                                                                          >  2:1809/1‑148 (MQ=255)                                                               tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCa                                                                                        <  7:391814/147‑1 (MQ=255)                                                               tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCAc                                                                                       <  8:9227/148‑1 (MQ=255)                                                                 ggACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCg                                                                                     <  3:100159/148‑1 (MQ=255)                                                                    ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                 <  2:204273/149‑1 (MQ=255)                                                                    ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                 <  4:365369/149‑1 (MQ=255)                                                                        gACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGa                                                                             <  2:136605/149‑1 (MQ=255)                                                                         aCATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGaa                                                                            <  1:382482/149‑1 (MQ=255)                                                                              cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                               >  4:259319/1‑125 (MQ=255)                                                                              cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                               <  3:259319/125‑1 (MQ=255)                                                                               ccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                      >  4:97895/1‑149 (MQ=255)                                                                               ccAAGCCCGCAGCGCCCGGGCCTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                      >  3:311530/1‑149 (MQ=255)                                                                                cAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTcc                                                                     <  8:371400/149‑1 (MQ=255)                                                                                     ccGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGc                                                                <  1:211257/149‑1 (MQ=255)                                                                                       gCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCtt                                                              <  4:76312/149‑1 (MQ=255)                                                                                         aGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgc                                                            <  4:110189/149‑1 (MQ=255)                                                                                          gcgcCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgca                                                           <  3:97895/149‑1 (MQ=255)                                                                                               cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                      <  6:45477/149‑1 (MQ=255)                                                                                               cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                      <  5:277718/149‑1 (MQ=255)                                                                                                        cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                             <  5:34767/149‑1 (MQ=255)                                                                                                        cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                             <  4:311530/149‑1 (MQ=255)                                                                                                        cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTg                                              <  4:398010/148‑1 (MQ=255)                                                                                                              ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                       <  7:225935/149‑1 (MQ=255)                                                                                                              ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                       <  6:44603/149‑1 (MQ=255)                                                                                                              ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                       >  1:380645/1‑149 (MQ=255)                                                                                                                aTGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGc                                     >  4:396882/1‑149 (MQ=255)                                                                                                                      tCGGCCCGGAAGGCCAGCAACTGCCGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                               >  4:11584/1‑149 (MQ=255)                                                                                                                      tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGc                                           <  6:410171/137‑1 (MQ=38)                                                                                                                      tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                               >  6:5560/1‑149 (MQ=38)                                                                                                                      tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCAGGCGCTTGCAGCAGATCGGCCTGGGc                                           >  5:410171/1‑137 (MQ=37)                                                                                                                      gcGGCCCTGAAGGCCAGCTACTGCTGGCCCTGCGGGCCGACTAGCGGCGCCTGGGCCAGGACTTCTCGATCAGTGTTGCTCAGGCCTATTCACCGGTTCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                               <  8:400796/148‑1 (MQ=255)                                                                                                                        ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                              <  7:75375/148‑1 (MQ=255)                                                                                                                        ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGgc                             >  3:363566/1‑149 (MQ=255)                                                                                                                        ggCCCGGAAGGACAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                              <  4:100466/148‑1 (MQ=255)                                                                                                                         gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                                >  6:271655/1‑145 (MQ=255)                                                                                                                         gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                                <  5:271655/145‑1 (MQ=255)                                                                                                                          cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                   >  2:305565/1‑125 (MQ=255)                                                                                                                          cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                   <  1:305565/125‑1 (MQ=255)                                                                                                                               agggCCAGCAGTTGCTGGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATc                      >  8:227546/3‑149 (MQ=255)                                                                                                                                  gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                         <  1:411227/111‑1 (MQ=255)                                                                                                                                  gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                         >  2:411227/1‑111 (MQ=255)                                                                                                                                    cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                 >  1:299019/1‑149 (MQ=255)                                                                                                                                    cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                 >  5:35451/1‑149 (MQ=255)                                                                                                                                    cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                 <  1:309993/149‑1 (MQ=255)                                                                                                                                      gCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTc               >  7:301875/1‑149 (MQ=255)                                                                                                                                        aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg             >  7:314469/1‑149 (MQ=255)                                                                                                                                        aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg             <  8:261421/149‑1 (MQ=255)                                                                                                                                         gttgctgGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTgg            <  7:227546/147‑1 (MQ=255)                                                                                                                                           tgctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg          >  4:210896/1‑149 (MQ=255)                                                                                                                                             ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                       >  8:369652/1‑118 (MQ=255)                                                                                                                                             ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                       <  7:369652/118‑1 (MQ=255)                                                                                                                                             ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg          <  4:3429/147‑1 (MQ=255)                                                                                                                                              tgGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtgctg       <  2:319088/149‑1 (MQ=255)                                                                                                                                                  ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGTGCTGATCCTGATTCTGGTGCTGCAgc   <  8:25632/149‑1 (MQ=255)                                                                                                                                                  ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCa     >  1:344274/1‑147 (MQ=255)                                                                                                                                                  ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgc   <  1:1809/149‑1 (MQ=255)                                                                                                                                                    tGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgcg  >  7:366527/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGTTTTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGCG  >  NC_002947/2677883‑2678177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑