Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,678,034	T→G	6.1%	R125R (CGT→CGG)	PP_2348 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,678,034	0	T	G	6.1%	288.2 / 6.7	93	R125R (CGT→CGG)	PP_2348	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (41/46);  new base G (2/4);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGCGC  >  NC_002947/2677887‑2678178                                                                                                                                                    |                                                                                                                                                 ttCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGt                                                                                                                                                  <  5:6507/148‑1 (MQ=255)    cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                               >  1:204273/1‑148 (MQ=255)    cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCg                                                                                                                                              >  3:365369/1‑149 (MQ=255)    cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCg                                                                                                                                              <  5:270740/149‑1 (MQ=38)    cGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGCTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGcc                                                                                                                                               <  4:211693/148‑1 (MQ=255)     ggTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGa                                                                                                                                             <  3:199919/149‑1 (MQ=255)       ttACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGAc                                                                                                                                            >  3:3429/1‑148 (MQ=255)         actactTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTAc                                                                                                                                         >  8:391814/1‑149 (MQ=255)             ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                     <  5:221239/149‑1 (MQ=255)             ctTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgc                                                                                                                                     <  5:74509/149‑1 (MQ=255)              ttCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGgcg                                                                                                                                    >  6:10178/1‑149 (MQ=255)                 cGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCt                                                                                                                                 >  4:100159/1‑149 (MQ=255)                   cATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTgg                                                                                                                               >  1:414223/1‑149 (MQ=255)                        aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          >  4:13344/1‑149 (MQ=255)                        aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          <  5:10178/149‑1 (MQ=255)                        aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          >  5:45477/1‑149 (MQ=255)                        aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          <  6:331757/149‑1 (MQ=255)                        aTTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          >  8:75375/1‑149 (MQ=255)                         tttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAg                                                                                                                          >  2:222147/1‑148 (MQ=255)                          ttCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCACGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGa                                                                                                                        >  8:225935/1‑149 (MQ=255)                                cAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACAtctc                                                                                                                  >  3:398010/1‑149 (MQ=255)                                  gggTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCg                                                                                                                 >  2:211257/1‑148 (MQ=255)                                     tACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCa                                                                                                             <  8:204503/149‑1 (MQ=255)                                        cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                          >  5:44603/1‑149 (MQ=255)                                        cTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAgtg                                                                                                          >  3:76312/1‑149 (MQ=255)                                           gAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTg                                                                                                       <  3:314940/149‑1 (MQ=255)                                              ggctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                    <  4:35194/147‑1 (MQ=255)                                              gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCt                                                                                                     >  2:309993/1‑148 (MQ=255)                                              gTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTc                                                                                                    >  6:34767/1‑149 (MQ=255)                                                ctcGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGTCATCTCGATCAGTGTTGCTCAg                                                                                                  <  7:273495/149‑1 (MQ=255)                                                   gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTATCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                  <  3:310109/146‑1 (MQ=255)                                                   gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                        <  1:280123/140‑1 (MQ=255)                                                   gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGtt                                                                                                        >  2:280123/1‑140 (MQ=255)                                                   gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAg                                                                                                  <  2:414223/146‑1 (MQ=255)                                                   gTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGAc                                                                                               >  3:110189/1‑149 (MQ=255)                                                     tCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTa                                                                                             >  7:371400/1‑149 (MQ=255)                                                        cTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTAtt                                                                                           >  2:1809/1‑148 (MQ=255)                                                           tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCa                                                                                         <  7:391814/147‑1 (MQ=255)                                                           tgGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCAc                                                                                        <  8:9227/148‑1 (MQ=255)                                                             ggACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCg                                                                                      <  3:100159/148‑1 (MQ=255)                                                                ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                  <  2:204273/149‑1 (MQ=255)                                                                ccTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTgc                                                                                  <  4:365369/149‑1 (MQ=255)                                                                    gACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGa                                                                              <  2:136605/149‑1 (MQ=255)                                                                     aCATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGaa                                                                             <  1:382482/149‑1 (MQ=255)                                                                          cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                                >  4:259319/1‑125 (MQ=255)                                                                          cccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGa                                                                                                <  3:259319/125‑1 (MQ=255)                                                                           ccAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                       >  4:97895/1‑149 (MQ=255)                                                                           ccAAGCCCGCAGCGCCCGGGCCTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTc                                                                       >  3:311530/1‑149 (MQ=255)                                                                            cAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTcc                                                                      <  8:371400/149‑1 (MQ=255)                                                                                 ccGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGc                                                                 <  1:211257/149‑1 (MQ=255)                                                                                   gCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCtt                                                               <  4:76312/149‑1 (MQ=255)                                                                                     aGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgc                                                             <  4:110189/149‑1 (MQ=255)                                                                                      gcgcCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgca                                                            <  3:97895/149‑1 (MQ=255)                                                                                           cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                       <  5:277718/149‑1 (MQ=255)                                                                                           cGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGa                                                       <  6:45477/149‑1 (MQ=255)                                                                                                    cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                              <  5:34767/149‑1 (MQ=255)                                                                                                    cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTgg                                              <  4:311530/149‑1 (MQ=255)                                                                                                    cGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTg                                               <  4:398010/148‑1 (MQ=255)                                                                                                          ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                        <  6:44603/149‑1 (MQ=255)                                                                                                          ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                        >  1:380645/1‑149 (MQ=255)                                                                                                          ccATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                        <  7:225935/149‑1 (MQ=255)                                                                                                            aTGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGc                                      >  4:396882/1‑149 (MQ=255)                                                                                                                  tCGGCCCGGAAGGCCAGCAACTGCCGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                                >  4:11584/1‑149 (MQ=255)                                                                                                                  tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGc                                            <  6:410171/137‑1 (MQ=38)                                                                                                                  tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                                >  6:5560/1‑149 (MQ=38)                                                                                                                  tCGGCACGCAGGGCCAGCAGTTGCTGGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCAGGCGCTTGCAGCAGATCGGCCTGGGc                                            >  5:410171/1‑137 (MQ=37)                                                                                                                  gcGGCCCTGAAGGCCAGCTACTGCTGGCCCTGCGGGCCGACTAGCGGCGCCTGGGCCAGGACTTCTCGATCAGTGTTGCTCAGGCCTATTCACCGGTTCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtg                                <  8:400796/148‑1 (MQ=255)                                                                                                                    ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                               <  7:75375/148‑1 (MQ=255)                                                                                                                    ggCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGgc                              >  3:363566/1‑149 (MQ=255)                                                                                                                    ggCCCGGAAGGACAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggtgg                               <  4:100466/148‑1 (MQ=255)                                                                                                                     gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                                 >  6:271655/1‑145 (MQ=255)                                                                                                                     gCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCtggt                                 <  5:271655/145‑1 (MQ=255)                                                                                                                      cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                    >  2:305565/1‑125 (MQ=255)                                                                                                                      cccGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCg                                                    <  1:305565/125‑1 (MQ=255)                                                                                                                           agggCCAGCAGTTGCTGGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATc                       >  8:227546/3‑149 (MQ=255)                                                                                                                              gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                          >  2:411227/1‑111 (MQ=255)                                                                                                                              gCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTgcagc                                                          <  1:411227/111‑1 (MQ=255)                                                                                                                                cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                  >  5:35451/1‑149 (MQ=255)                                                                                                                                cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                  <  1:309993/149‑1 (MQ=255)                                                                                                                                cAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGAt                  >  1:299019/1‑149 (MQ=255)                                                                                                                                  gCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTc                >  7:301875/1‑149 (MQ=255)                                                                                                                                    aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg              >  7:314469/1‑149 (MQ=255)                                                                                                                                    aaCTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTg              <  8:261421/149‑1 (MQ=255)                                                                                                                                     gttgctgGCCTTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTgg             <  7:227546/147‑1 (MQ=255)                                                                                                                                       tgctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg           >  4:210896/1‑149 (MQ=255)                                                                                                                                         ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                        <  7:369652/118‑1 (MQ=255)                                                                                                                                         ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATgg                                        >  8:369652/1‑118 (MQ=255)                                                                                                                                         ctgGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtg           <  4:3429/147‑1 (MQ=255)                                                                                                                                          tgGCCTTCCGGGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGtgctg        <  2:319088/149‑1 (MQ=255)                                                                                                                                              ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGTGCTGATCCTGATTCTGGTGCTGCAgc    <  8:25632/149‑1 (MQ=255)                                                                                                                                              ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCa      >  1:344274/1‑147 (MQ=255)                                                                                                                                              ccTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgc    <  1:1809/149‑1 (MQ=255)                                                                                                                                                tGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgcg   >  7:366527/1‑148 (MQ=255)                                                                                                                                                  cGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAgcgc  <  4:81702/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TTCCGGTTACTACTTCCGCATCGATTTCGACCAGGGTACCTGGAGGTCTCGTTCGCTGTGGGACCTGGACATGCCCAAGCCCGCAGCGCCCGGGCTTTCCGATAGCCATGAACTCGGCCCGGAAGGCCAGCAACTGCTGGCCCTGCGTGCCGACTACCGGCGCCTGGGCCAGGACATCTCGATCAGTGTTGCTCAGGACTATTCACCGGTGCGCGAAGGTTTCCGACGCTTGCAGCAGATCGGCCTGGGCATGGGCCTGGTGGCGCTGATCCTGATTCTGGTGCTGCAGCGC  >  NC_002947/2677887‑2678178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑