Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,699,833 G→C 5.7% P226P (CCC→CCG) ‡ fsr‑II ← fosmidomycin efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,699,8330GC 5.7% 223.5 / 2.6 70P226P (CCC→CCG) ‡fsr‑IIfosmidomycin efflux system
Reads supporting (aligned to +/- strand):  ref base G (33/33);  new base C (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ACAGTTGGCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGCGGAATGATAATGG  >  NC_002947/2699685‑2699978
                                                                                                                                                    |                                                                                                                                                 
aCAGTTGGCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCg                                                                                                                                                   >  6:444560/1‑149 (MQ=255)
 cAGTTGGCTTTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCg                                                                                                                                                   <  7:70861/148‑1 (MQ=255)
   gTTGGCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCgggg                                                                                                                                                <  6:429558/149‑1 (MQ=255)
    ttGGCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCggggg                                                                                                                                               >  3:295718/1‑149 (MQ=255)
       gCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCt                                                                                                                                            <  8:16467/149‑1 (MQ=255)
       gCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGACACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCt                                                                                                                                            >  7:179642/1‑149 (MQ=255)
            gctgcACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGcc                                                                                                                                             >  4:101449/1‑143 (MQ=255)
            gctgcACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGcc                                                                                                                                             <  3:101449/143‑1 (MQ=255)
            gctgcACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTgg                                                                                                                                       >  3:303318/1‑149 (MQ=255)
               gcACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGCTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCgg                                                                                                                                                  >  4:158934/1‑135 (MQ=255)
               gcACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGCTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCgg                                                                                                                                                  <  3:158934/135‑1 (MQ=255)
                     tCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAg                                                                                                                              >  5:138494/1‑149 (MQ=255)
                     tCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAg                                                                                                                              >  1:346730/1‑149 (MQ=255)
                           cGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCAGGGGGCCTGTTGGCCCGCGAAGGTCtt                                                                                                                         >  8:391436/1‑148 (MQ=255)
                             aaaTGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAg                                                                                                                      <  7:359048/149‑1 (MQ=255)
                                tGCTCGCTCATGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTCGGCCCGCGAAGGTCTTGAGCCg                                                                                                                   <  7:273332/149‑1 (MQ=255)
                                  cTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCggg                                                                                                                 <  6:232306/149‑1 (MQ=255)
                                     gATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCt                                                                                                              >  2:153754/1‑149 (MQ=255)
                                      aTCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGACCTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTg                                                                                                             <  4:74917/149‑1 (MQ=255)
                                           gTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTgg                                                                                                            >  6:326941/1‑145 (MQ=255)
                                           gTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTgg                                                                                                            <  5:326941/145‑1 (MQ=255)
                                           gTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCCCCGGCCTGtcgca                                                                                                                                      <  6:106090/119‑5 (MQ=255)
                                           gTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCCCCGGCCTGtcgca                                                                                                                                      >  5:106090/1‑115 (MQ=255)
                                              gAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGgc                                                                                                     <  8:179642/149‑1 (MQ=255)
                                                aGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGgcg                                                                                                    >  3:79359/1‑148 (MQ=255)
                                                 gttGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCa                                                                                                  <  5:284606/147‑1 (MQ=255)
                                                     aagaagTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCt                                                                                              >  2:41602/1‑149 (MQ=255)
                                                       gaagTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTcc                                                                                            >  5:9203/1‑149 (MQ=255)
                                                                   cGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCAccc                                                                                >  7:354530/1‑149 (MQ=255)
                                                                   cGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCAccc                                                                                >  4:196164/1‑149 (MQ=255)
                                                                      aGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCa                                                                             <  5:102361/149‑1 (MQ=255)
                                                                        cGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGt                                                                           <  8:363714/149‑1 (MQ=255)
                                                                            gTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCg                                                                                      <  7:115503/134‑1 (MQ=255)
                                                                            gTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCg                                                                                      >  8:115503/1‑134 (MQ=255)
                                                                             tAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCGGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCa                                                                       <  1:307785/148‑1 (MQ=255)
                                                                             tAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCNCACGCTCCAGCCCGTCACCCGCAGTAGCAc                                                                      <  1:41602/149‑1 (MQ=255)
                                                                             tAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCa                                                                       <  4:20314/148‑1 (MQ=255)
                                                                               gACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCAc                                                                      <  2:346730/147‑1 (MQ=255)
                                                                                aCGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCAc                                                                      >  1:414996/1‑146 (MQ=255)
                                                                                aCGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCAc                                                                      >  2:129722/1‑146 (MQ=255)
                                                                                aCGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAg                                                                   <  5:444560/149‑1 (MQ=255)
                                                                                    aCTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAAca                                                               <  4:295718/149‑1 (MQ=255)
                                                                                    aCTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAAca                                                               <  1:436280/149‑1 (MQ=255)
                                                                                     cTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCCCCCGCAGTAGCCCCAGAAcag                                                              >  7:358828/1‑149 (MQ=255)
                                                                                        ggCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAAcagc                                                             <  3:196164/147‑1 (MQ=255)
                                                                                                tCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGCCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCAGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCcagcgcagcg                                                   >  3:306105/1‑149 (MQ=255)
                                                                                                   gCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGcc                                                >  7:220726/1‑149 (MQ=255)
                                                                                                   gCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGc                                                 >  4:22470/1‑148 (MQ=255)
                                                                                                    cACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCa                                               <  2:79179/149‑1 (MQ=255)
                                                                                                    cACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCACAGTAGCACCAGAAcagc                                                             >  8:219702/1‑135 (MQ=255)
                                                                                                    cACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCACAGTAGCACCAGAAcagc                                                             <  7:219702/135‑1 (MQ=255)
                                                                                                      cGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAg                                              >  3:333278/1‑148 (MQ=255)
                                                                                                       ggCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCa                                            <  7:391436/149‑1 (MQ=255)
                                                                                                         cgctCACAGCCACAGCGCGCCAGATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGGCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATg                                          <  6:279123/146‑1 (MQ=255)
                                                                                                            tCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAAc                                       >  1:291971/1‑149 (MQ=255)
                                                                                                               ccGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAg                                    >  6:416897/1‑149 (MQ=255)
                                                                                                                cGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAgg                                   <  4:303318/149‑1 (MQ=255)
                                                                                                                     caGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATg                              <  4:79359/149‑1 (MQ=255)
                                                                                                                       gcgcgcCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGc                            <  6:9203/149‑1 (MQ=255)
                                                                                                                       gcgcgcCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGc                            <  8:354530/149‑1 (MQ=255)
                                                                                                                            ccACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGtt                       <  5:101350/149‑1 (MQ=255)
                                                                                                                              acaTGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTgg                     >  2:240817/1‑149 (MQ=255)
                                                                                                                              acaTGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTgg                     >  4:360131/1‑149 (MQ=255)
                                                                                                                              acaTGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTgg                     >  3:279023/1‑149 (MQ=255)
                                                                                                                               caTGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGc                    >  4:271768/1‑149 (MQ=255)
                                                                                                                                aTGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGcc                   >  8:4376/1‑149 (MQ=255)
                                                                                                                                  ggCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGc                            <  3:86892/138‑1 (MQ=255)
                                                                                                                                  ggCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGc                            >  4:86892/1‑138 (MQ=255)
                                                                                                                                    cACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGTAGGTCTTGAGGCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGc               <  4:306105/149‑1 (MQ=255)
                                                                                                                                    cACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGc               >  7:412053/1‑149 (MQ=255)
                                                                                                                                         gccaaCAGGCCCCCGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGtt                       <  2:99439/132‑1 (MQ=255)
                                                                                                                                         gccaaCAGGCCCCCGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGtt                       >  1:99439/5‑136 (MQ=255)
                                                                                                                                                 gCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCATCCCGTCACCCGCAGTAGCACCAGACCAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGCGGAATGATAATgg  <  7:19209/149‑1 (MQ=255)
                                                                                                                                                 gCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGCGGAATGATAATgg  >  1:259771/1‑149 (MQ=255)
                                                                                                                                                   cGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGcc                   <  2:52682/130‑1 (MQ=255)
                                                                                                                                                   cGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGcc                   >  1:52682/1‑130 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAGTTGGCTGTGCTGCACACTCAGGCCGAAATGCTCGATCAGGTAGAAGGTGAAGAAGTTGGTGAACGAAGCGATGTAGACGAACTTGGCGAACATCAGCACGGCGATCACCGCCACAGCGCGCCACATGGCACCCTGCGACAGGCCGGGGGCCTGTTGGCCCGCGAAGGTCTTGAGCCGGGCCTGGCCGTGGCGCACGCTCCAGCCCGTCACCCGCAGTAGCACCAGAACAGCCAGCGCAGCGGCCAGCATGAACCAGGCAATGGCCGGTTGGCCGTGCGGAATGATAATGG  >  NC_002947/2699685‑2699978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑