Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,703,428 T→G 7.0% A195A (GCT→GCG)  PP_2369 → leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,703,4280TG 7.0% 188.6 / 2.6 65A195A (GCT→GCG) PP_2369leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (35/24);  new base G (3/2);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTAAGTGCGCAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCG  >  NC_002947/2703280‑2703576
                                                                                                                                                     |                                                                                                                                                    
cGCTAAGTGCGCAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTtgct                                                                                                                                                      <  1:411377/149‑1 (MQ=255)
 gCTAAGTGCGCAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTtgctg                                                                                                                                                     <  6:436731/149‑1 (MQ=255)
       tGCGCAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcg                                                                                                                                               >  3:399523/1‑149 (MQ=255)
          gcAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcgccg                                                                                                                                            >  6:257963/1‑149 (MQ=255)
           cAAGGCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTTAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGTGGGCCAACCTGGCGCTTGCTGCAATcgccgc                                                                                                                                           <  8:261065/149‑1 (MQ=255)
           cAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTTGATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcgccg                                                                                                                                            >  1:315142/1‑149 (MQ=255)
           cAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATAAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcgccg                                                                                                                                            >  1:34869/1‑148 (MQ=255)
            aaGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcgccgc                                                                                                                                           >  4:139506/1‑148 (MQ=255)
            aaGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGA‑GGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATcgccgc                                                                                                                                           >  6:114610/1‑147 (MQ=255)
              gCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGcaag                                                                                                                                        <  3:207195/149‑1 (MQ=255)
              gCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGATTGAAGGCGAGGGGGGGGCCGGCGAGAGAACCGTGGAGGAGGCGTTGAGGGCAAACCTGCCGATTGATGCAATCGCCGcaag                                                                                                                                        >  2:214137/1‑149 (MQ=255)
                cTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGcaagga                                                                                                                                      >  8:289507/1‑149 (MQ=255)
                 tttGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATAGCAGcaaggat                                                                                                                                     >  5:151287/1‑149 (MQ=255)
                       cGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGcaaggatcaagga                                                                                                                               <  2:315142/149‑1 (MQ=255)
                        gCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGcaaggatcaaggat                                                                                                                              >  1:63518/1‑149 (MQ=255)
                                 gagaCATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGAt                                                                                                                     <  4:399523/149‑1 (MQ=255)
                                     cATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAg                                                                                                                  <  4:106088/148‑1 (MQ=255)
                                          atatTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGAcc                                                                                                            >  8:45968/1‑149 (MQ=255)
                                           tatTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGAcat                                                                                                           >  5:351623/1‑147 (MQ=255)
                                           tatTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCt                                                                                                           >  6:200500/1‑149 (MQ=255)
                                           tatTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCt                                                                                                           >  7:8522/1‑149 (MQ=255)
                                             ttGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAAAAACCTTGAGGAGGCGTTTAGGGCCAACCTTGCCCTTGCTTCAATCGCCGCAAGGATCAAGGATTAAATAGAAGAGCAGACCTGt                                                                                                         >  1:347547/1‑149 (MQ=255)
                                                      gTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGAt                                                                                                 >  1:227728/1‑148 (MQ=255)
                                                          aTCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAAc                                                                                             >  5:195871/1‑149 (MQ=255)
                                                          aTCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAAc                                                                                             >  6:114069/1‑149 (MQ=255)
                                                            cAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAAc                                                                                             >  8:27614/1‑147 (MQ=255)
                                                              aaGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAgg                                                                                         >  5:262289/1‑149 (MQ=255)
                                                               aGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGc                                                                                        >  5:32546/1‑149 (MQ=255)
                                                                        cAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGAc                                                                                                             <  6:389581/119‑1 (MQ=255)
                                                                        cAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGAc                                                                                                             >  5:389581/1‑119 (MQ=255)
                                                                              aaGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTc                                                                         >  7:125562/1‑149 (MQ=255)
                                                                                  ttGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTc                                                                     >  2:410523/1‑149 (MQ=255)
                                                                                    gAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTcc                                                                    <  5:257963/148‑1 (MQ=255)
                                                                                     aaGGCGATGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCt                                                                   <  7:367582/148‑1 (MQ=255)
                                                                                     aaGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCt                                                                   <  3:139506/148‑1 (MQ=255)
                                                                                     aaGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTg                                                                  >  2:202442/1‑149 (MQ=255)
                                                                                           aGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGcc                                                            >  1:282465/1‑149 (MQ=255)
                                                                                                   gCCGTCCAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTCCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACACCGTCTTTCCTGCCAGCCGACAACGc                                                    >  8:44560/1‑149 (MQ=255)
                                                                                                      gTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCgt                                                 >  3:273369/1‑149 (MQ=255)
                                                                                                         gagaGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCgtgt                                               <  7:375442/148‑1 (MQ=255)
                                                                                                              cACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCgt                                                 <  6:225233/141‑1 (MQ=255)
                                                                                                              cACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCgt                                                 >  5:225233/1‑141 (MQ=255)
                                                                                                                cccTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAgtgt                                       <  5:114069/149‑1 (MQ=255)
                                                                                                                 ccTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCTACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTc                                      <  5:114610/149‑1 (MQ=255)
                                                                                                                  cTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTca                                     <  7:45968/149‑1 (MQ=255)
                                                                                                                     gaggagGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCacac                                  >  1:88838/1‑149 (MQ=255)
                                                                                                                           gCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCg                            >  1:314945/1‑149 (MQ=255)
                                                                                                                           gCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCg                            <  1:202442/149‑1 (MQ=255)
                                                                                                                            cGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGGCGTGTTGAAGTGTCACACCCTGCgg                           >  6:50854/1‑149 (MQ=255)
                                                                                                                              ttGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCt                         >  6:78613/1‑149 (MQ=255)
                                                                                                                                  gggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTg                     <  2:358674/149‑1 (MQ=255)
                                                                                                                                   ggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTgg                    <  8:125562/149‑1 (MQ=255)
                                                                                                                                   ggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTgg                    <  8:8522/149‑1 (MQ=255)
                                                                                                                                   ggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTgg                    <  6:262289/149‑1 (MQ=255)
                                                                                                                                   gaccAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTcac                                    <  6:45092/131‑1 (MQ=255)
                                                                                                                                   gaccAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTcac                                    >  5:45092/3‑133 (MQ=255)
                                                                                                                                    gCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTggg                   <  2:63518/149‑1 (MQ=255)
                                                                                                                                      cAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGa                 <  6:195871/149‑1 (MQ=255)
                                                                                                                                        ttgcggCGATTGCAGCAAGCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAag               >  7:240956/5‑149 (MQ=255)
                                                                                                                                             gcgcTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAaggag            <  1:214137/147‑1 (MQ=255)
                                                                                                                                              tccTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGt          >  4:264141/3‑148 (MQ=255)
                                                                                                                                              cgcTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTc         <  6:32546/149‑1 (MQ=255)
                                                                                                                                               gcTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCa        >  1:404042/1‑149 (MQ=255)
                                                                                                                                                cTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAaggag            <  6:413891/144‑1 (MQ=255)
                                                                                                                                                cTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAaggag            >  5:413891/1‑144 (MQ=255)
                                                                                                                                                 ttGCGGCGATTGCAGCAAGGATCAAGTATGAAATCGATGAGCAGAGCTGTCAGTGTATCAAGCAGGCGGTTGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACTCCCTGCg                            <  4:14069/127‑1 (MQ=255)
                                                                                                                                                 ttGCGGCGATTGCAGCAAGGATCAACGATGAAATCGATGAGCAGACCTGTCAGTGGAACAACCAGGCCGTCGGCACAGCCTCTTTCCTGCCAGCCGACAACGCCCTGTTGAAGTGTCACACACTGCg                            >  3:14069/1‑127 (MQ=255)
                                                                                                                                                   gctgcAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGAACTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTg     <  6:151287/148‑1 (MQ=255)
                                                                                                                                                     tgcAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGGGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGgcg  >  4:362828/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTAAGTGCGCAAGCCTTTGTCCGCCTTTGCAGAGACATTGATATTGGCAGGCGTT‑ATCAAAGCCATTTGCAATCGAAGCTTGAAGGCGAGGGGGGGGCCGTCGAGAGCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCG  >  NC_002947/2703280‑2703576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑