Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,744,519 C→T 6.2% Q20* (CAG→TAG)  PP_2399 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,744,5190CT 6.2% 193.0 / 2.9 64Q20* (CAG→TAG) PP_2399hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base T (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGATTTCAACTATCCGCCCTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAGTAC  >  NC_002947/2744376‑2744666
                                                                                                                                               |                                                                                                                                                    
tGATTTCAACTATCCGCCCTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAgcgc                                                                                                                                                 <  8:241562/149‑1 (MQ=255)
        aCTATCCGCCCTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGTGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGcccc                                                                                                                                         <  3:313239/149‑1 (MQ=255)
               gCCCTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGccc                                                                                                                                 <  4:167992/149‑1 (MQ=255)
                  cTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCATAGCGCCATGCCCCG‑CCGCCCGGc                                                                                                                              >  3:337715/1‑149 (MQ=255)
                  cTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCATAGCGCCATGCCCCG‑CCGCCCGGc                                                                                                                              >  3:343481/1‑149 (MQ=255)
                      gCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTc                                                                                                                           <  4:386126/148‑1 (MQ=255)
                         gAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAgg                                                                                                                       <  5:344459/149‑1 (MQ=255)
                          aaCTGTTGGCCGAGACGGTGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGc                                                                                                                      >  1:254968/1‑149 (MQ=255)
                             tGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCg                                                                                                                   >  8:223754/1‑149 (MQ=255)
                               ttGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCgg                                                                                                                  <  8:81789/148‑1 (MQ=255)
                                   ccGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCt                                                                                                             <  2:68909/149‑1 (MQ=255)
                                   ccGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCt                                                                                                             >  3:79679/1‑149 (MQ=255)
                                    cGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCt                                                                                                             >  8:335516/1‑148 (MQ=255)
                                       gaCGGCGCGTATGCGTGCCGTGCAACGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAg                                                                                                         <  7:123441/149‑1 (MQ=255)
                                              cgTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTAcc                                                                                                  <  2:218103/149‑1 (MQ=255)
                                                 aTGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGt                                                                                                >  2:297528/1‑148 (MQ=255)
                                                  tGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTTGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGa                                                                                              <  3:297211/149‑1 (MQ=255)
                                                  tGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCGCCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGt                                                                                                <  1:297528/148‑1 (MQ=255)
                                                     gtgccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgtt                                                                                           >  4:54846/1‑149 (MQ=255)
                                                     gtgccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgtt                                                                                           >  2:210291/1‑149 (MQ=255)
                                                       gccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGa                                                                                                                         <  8:384252/117‑1 (MQ=255)
                                                       gccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGa                                                                                                                         >  7:384252/1‑117 (MQ=255)
                                                        ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                        <  3:106292/149‑1 (MQ=255)
                                                        ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                        <  4:79679/149‑1 (MQ=255)
                                                        ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                        <  7:223754/149‑1 (MQ=255)
                                                        ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgt                                                                                         >  2:254403/1‑148 (MQ=255)
                                                             cAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATg                                                                                   >  2:278793/1‑149 (MQ=255)
                                                                        cGGAGTGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGaa                                                                        <  2:135851/149‑1 (MQ=255)
                                                                        cGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGaa                                                                        <  7:185933/149‑1 (MQ=255)
                                                                          gAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCATAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAgg                                                                      <  4:343481/149‑1 (MQ=255)
                                                                          gAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCATAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAgg                                                                      <  4:337715/149‑1 (MQ=255)
                                                                            gggCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCt                                                                    >  6:136538/1‑149 (MQ=255)
                                                                              gCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATTGCGACATCGAAGCCTTg                                                                  >  8:214609/1‑149 (MQ=255)
                                                                               cAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgc                                                                 <  6:108731/149‑1 (MQ=255)
                                                                                aaGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgct                                                                >  5:434127/1‑149 (MQ=255)
                                                                                  gCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctgg                                                              <  3:54846/149‑1 (MQ=255)
                                                                                  gCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctgg                                                              >  6:171229/1‑149 (MQ=255)
                                                                                   cAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctggc                                                             <  5:136538/149‑1 (MQ=255)
                                                                                    aGATGTATGAGACCCTGCGTTCGCATCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAATGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctggct                                                            <  7:214609/149‑1 (MQ=255)
                                                                                           tgaGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCt                                                     >  4:33575/1‑149 (MQ=255)
                                                                                           tgaGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCt                                                     >  4:27951/1‑149 (MQ=255)
                                                                                                 ccTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCgg                                               <  2:429077/149‑1 (MQ=255)
                                                                                                 ccTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCgg                                               <  5:254642/149‑1 (MQ=255)
                                                                                                   tgcgtgcgCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGt                                              >  8:99031/1‑148 (MQ=255)
                                                                                                       tgcgCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATgc                                         >  5:18998/1‑149 (MQ=255)
                                                                                                          gcagcaGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATgcgc                                       >  6:8336/1‑148 (MQ=255)
                                                                                                                 tATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGtt                               >  5:81104/1‑149 (MQ=255)
                                                                                                                    gCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTggg                            >  2:297337/1‑149 (MQ=255)
                                                                                                                     ccTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTggg                            <  3:282430/148‑1 (MQ=255)
                                                                                                                     ccTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGc                           <  3:33575/149‑1 (MQ=255)
                                                                                                                     ccTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGc                           <  3:27951/149‑1 (MQ=255)
                                                                                                                        tGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGTGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGc                           >  8:268569/1‑146 (MQ=255)
                                                                                                                          gCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGagc                       <  4:84416/148‑1 (MQ=255)
                                                                                                                           cccGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGagcag                     >  1:11059/1‑149 (MQ=255)
                                                                                                                             cGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAgcg                   >  6:13126/1‑149 (MQ=255)
                                                                                                                                 aCCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTgg               >  4:73600/1‑149 (MQ=255)
                                                                                                                                  ccTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTgg               >  2:247459/1‑148 (MQ=255)
                                                                                                                                      cgcgACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGc          >  7:153797/1‑149 (MQ=255)
                                                                                                                                          aCCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAg                                                                       <  7:247233/84‑1 (MQ=255)
                                                                                                                                          aCCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTCGCATGccc        >  8:3708/1‑147 (MQ=255)
                                                                                                                                          aCCCACAGCGCCATGCCCCG‑CCGCACGGCGTCCAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAg                                                                       >  8:247233/1‑84 (MQ=255)
                                                                                                                                            ccACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTGTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCACGGTGCTGCGCCAGGCGTTGGGCGAGCCGCGCTGGCATGCCCGAg     >  4:283814/1‑148 (MQ=255)
                                                                                                                                            ccACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAg     >  7:345105/1‑148 (MQ=255)
                                                                                                                                            ccACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTGCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAg     >  3:381635/1‑148 (MQ=255)
                                                                                                                                              acaGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGTGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAGTAc  <  7:268569/149‑1 (MQ=255)
                                                                                                                                              acaGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGGCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAg     <  3:136124/146‑1 (MQ=255)
                                                                                                                                              acaGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAg     >  4:136124/1‑146 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGATTTCAACTATCCGCCCTTGGCCGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCCATGCCCCG‑CCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCGCTGGCATGCCCGAGTAC  >  NC_002947/2744376‑2744666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑