Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,776,841	T→G	5.0%	V396V (GTT→GTG) ‡	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,776,841	0	T	G	5.0%	252.2 / 3.8	80	V396V (GTT→GTG) ‡	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (35/41);  new base G (2/2);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAA  >  NC_002947/2776695‑2776988                                                                                                                                                   |                                                                                                                                                    gtgGCCATGGCGCTGTTCTCGGGTTTCTACAACATCGGCATTGGCGGTTGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTg                                                                                                                                                    <  7:169981/148‑1 (MQ=255)  tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTTGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGcc                                                                                                                                                  <  6:75491/149‑1 (MQ=255)   ggCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATAGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                   >  1:349485/1‑147 (MQ=255)    gCCTTGGCGCTGTTCTCGGGTATCTACATCATCGGCTTTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGGCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                                 <  2:431864/148‑1 (MQ=255)    gCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                                 <  8:349221/148‑1 (MQ=255)    gCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAg                                                                                                                                                >  2:21519/1‑149 (MQ=255)    gCCATGGCGCTGTTCTCGGGTATCTACAACATAGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAg                                                                                                                                                >  1:266950/1‑149 (MQ=255)      cATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTa                                                                                                                                              <  7:398270/149‑1 (MQ=255)      cATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTa                                                                                                                                              <  3:113504/149‑1 (MQ=255)          gcgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCg                                                                                                                                          >  2:56302/1‑149 (MQ=255)          gcgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAcca                                                                                                                                           <  6:35276/148‑2 (MQ=255)          gcgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAcca                                                                                                                                           <  7:129319/148‑2 (MQ=255)          gcgcTGTTCTCGGGTATCTACAACAGCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCg                                                                                                                                          <  2:181240/149‑1 (MQ=255)           cgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAcccag                                                                                                                                         <  3:250399/149‑3 (MQ=255)           cgcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCgg                                                                                                                                         <  8:44607/149‑1 (MQ=255)            gcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                        <  4:86544/149‑1 (MQ=255)                     gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTTGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGtt                                                                                                                                                     <  3:17226/127‑1 (MQ=255)                     gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGtt                                                                                                                                                     >  4:17226/1‑127 (MQ=255)                      ggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                                >  7:334767/1‑147 (MQ=255)                        tATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAgg                                                                                                                            <  4:214916/149‑1 (MQ=255)                            tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCATTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                        >  8:145684/1‑149 (MQ=255)                            tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                        >  4:216377/1‑149 (MQ=255)                            tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                        >  5:334771/1‑149 (MQ=255)                             acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCATTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                       <  7:145684/149‑1 (MQ=255)                             acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAccc                                                                                                                                           >  8:377826/1‑129 (MQ=255)                             acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAccc                                                                                                                                           <  7:377826/129‑1 (MQ=255)                              caacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                        <  6:365220/147‑1 (MQ=255)                                      gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGa                                                                                                              <  7:249595/149‑1 (MQ=255)                                       cATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                             >  3:345433/1‑149 (MQ=255)                                        aTTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGc                                                                                                            <  1:21519/149‑1 (MQ=255)                                            gCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCTGTGGTCGGGCTGGTACTGGCCGTTGCCAGTCCGCGGCGGTTTAGCGAGGAGTTGAACCGTTGAGCCGat                                                                                                        >  8:373745/1‑148 (MQ=255)                                            gCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGGTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAg                                                                                                        <  3:58436/149‑1 (MQ=255)                                             cGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAgg                                                                                                       <  7:394136/149‑1 (MQ=255)                                              ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGacac                                                                                                                   >  8:317540/1‑136 (MQ=255)                                              ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGacac                                                                                                                   <  7:317540/136‑1 (MQ=255)                                              ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGc                                                                                                      <  6:334771/149‑1 (MQ=255)                                                tggtgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                         >  6:165213/1‑144 (MQ=255)                                                tggtgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                         <  5:165213/144‑1 (MQ=255)                                                   tgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTc                                                                                                 <  2:349485/149‑1 (MQ=255)                                                       ctgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGc                                                                                             <  8:334767/149‑1 (MQ=255)                                                            gggCAGTATCGTCAGCAGCAATATGGATGTGGCGGATATCGGCCTGGTGGGAGGCAGCGTGGCAGTGGTCGGGCTGTTACTGGCCGTTGCAAGTACCCGGCGATTTCGCGAGGCTTTGAAACGTAGAGCCGAAGCAGCTCATGCCCCtt                                                                                        >  8:385142/1‑149 (MQ=255)                                                             ggCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTTCTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTg                                                                                       >  1:140160/1‑149 (MQ=255)                                                                      gTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                              >  6:282676/1‑149 (MQ=255)                                                                       tCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                              >  5:162179/1‑148 (MQ=255)                                                                       tCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                              >  4:295585/1‑148 (MQ=255)                                                                       tCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                              >  5:155206/1‑148 (MQ=255)                                                                          gcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                          <  6:155206/149‑1 (MQ=255)                                                                          gcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                          <  6:162179/149‑1 (MQ=255)                                                                           cagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTagag                                                                         <  3:335878/149‑1 (MQ=255)                                                                           cagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTagag                                                                         <  6:63742/149‑1 (MQ=255)                                                                            agcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAgg                                                                        >  4:11396/1‑149 (MQ=255)                                                                                      atgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCaa                                                              <  3:295585/149‑1 (MQ=255)                                                                                      atgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCaa                                                              >  2:362662/1‑149 (MQ=255)                                                                                          ggCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACt                                                          >  4:76517/1‑149 (MQ=255)                                                                                           gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCTGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                         <  3:11396/149‑1 (MQ=255)                                                                                           gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAg                                                                   >  7:40472/1‑139 (MQ=255)                                                                                           gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAg                                                                   <  8:40472/139‑1 (MQ=255)                                                                                           gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                         <  5:282676/149‑1 (MQ=255)                                                                                            cGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                         >  6:236291/1‑148 (MQ=255)                                                                                            cGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                         >  6:84459/1‑148 (MQ=255)                                                                                             ggATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCa                                                       >  4:236223/1‑149 (MQ=255)                                                                                              gATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAg                                                      <  1:362662/149‑1 (MQ=255)                                                                                                 accGGCCTTGGGCGCGGCATCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGACGCGTTGACACCTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGccc                                                   <  7:373745/147‑1 (MQ=255)                                                                                                   cGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCAt                                                 >  5:320622/1‑149 (MQ=255)                                                                                                     gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                               <  7:307627/149‑1 (MQ=255)                                                                                                     gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                               >  5:47353/1‑149 (MQ=255)                                                                                                          gtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGata                                          <  2:140160/149‑1 (MQ=255)                                                                                                          gtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGat                                           <  2:266950/148‑1 (MQ=255)                                                                                                            gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGatat                                         >  5:144330/1‑148 (MQ=255)                                                                                                             ggcggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCaa                                                                               <  6:140139/109‑1 (MQ=255)                                                                                                             ggcggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCaa                                                                               >  5:140139/1‑109 (MQ=255)                                                                                                              gcggcAGCGTGGCAGTGGTCCGGCTGGTACTCGCCCTTGCCAGTACCCGGAGCTTTCGCGAGGCTTTGACACGTTGAGCCGAGGCCGCTAAGCCCCCTTGTCGGTCAAGTAGAGGCTCAGGCAAATACTGCAGCCCATGCCGATATaaa                                      >  8:387147/1‑149 (MQ=255)                                                                                                                       tGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAcccc                             <  6:320622/149‑1 (MQ=255)                                                                                                                         gCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCg                           >  2:95511/1‑149 (MQ=255)                                                                                                                                 gcgccgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACt                   >  7:134142/7‑149 (MQ=255)                                                                                                                                    gCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAaca                     >  6:64287/1‑144 (MQ=255)                                                                                                                                    gCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAaca                     <  5:64287/144‑1 (MQ=255)                                                                                                                                     cgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt               >  6:232035/3‑149 (MQ=255)                                                                                                                                     cTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt               >  4:214822/1‑149 (MQ=255)                                                                                                                                            tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac        >  8:202518/1‑149 (MQ=255)                                                                                                                                              gCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac        <  3:214822/147‑1 (MQ=255)                                                                                                                                               ccGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGGCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCg     <  3:236223/149‑1 (MQ=255)                                                                                                                                                  ttGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCaa  <  1:95511/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAA  >  NC_002947/2776695‑2776988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑