Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,796,627 A→C 7.2% intergenic (+60/‑113) PP_t42 → / → PP_t43 tRNA‑Val/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,796,6270AC 7.2% 169.1 / 4.8 56intergenic (+60/‑113)PP_t42/PP_t43tRNA‑Val/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base C (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

GCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTT  >  NC_002947/2796479‑2796773
                                                                                                                                                    |                                                                                                                                                  
gcACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGa                                                                                                                                                    <  6:246538/149‑1 (MQ=255)
    cacaCGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTATTTCATTGTCCATTGCCGCGCGAGCgg                                                                                                                                                >  7:265792/1‑149 (MQ=255)
     acacGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCATTTGTGCCTACCAAATCGAAAAAGGGGTGATCCTAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCggg                                                                                                                                               <  4:359557/149‑1 (MQ=255)
           cAGGCACATAGCTCAGGTGGTTAGATCACCACCTTTACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTCCCGCTCGCGCGGCAAtgga                                                                                                                                         <  6:337318/149‑4 (MQ=18)
             ggCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgc                                                                                                                                       <  5:65583/149‑1 (MQ=255)
             ggCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgc                                                                                                                                       <  8:265792/149‑1 (MQ=255)
              gCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgcg                                                                                                                                      >  8:163257/1‑149 (MQ=255)
              gCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgcg                                                                                                                                      <  7:88986/149‑1 (MQ=255)
              gCACATAGCTCAGTTGCGTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgcg                                                                                                                                      <  4:62275/149‑1 (MQ=255)
               cacaTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCgg                                                                                                                                     <  7:163257/149‑1 (MQ=255)
               cacaTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTCCCGCTCGCGCGGCAAtggacaat                                                                                                                                     <  2:152735/149‑8 (MQ=18)
                  aTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCAt                                                                                                                                  >  1:345934/1‑149 (MQ=255)
                           ttGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCa                                                                                                                           >  6:442170/1‑147 (MQ=255)
                           ttGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCa                                                                                                                           >  7:236797/1‑147 (MQ=255)
                               ttAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAgcgc                                                                                                                     <  1:72078/149‑1 (MQ=255)
                                   agCACCACCTTGACATGGTGGGGGTCGTTGGTACGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCttt                                                                                                                  >  8:12289/1‑148 (MQ=255)
                                       ccaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTc                                                                                                             <  1:156570/149‑1 (MQ=255)
                                       ccaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTc                                                                                                             >  7:393660/1‑149 (MQ=255)
                                       ccaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTc                                                                                                             <  8:174322/149‑1 (MQ=255)
                                        caccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCg                                                                                                            <  8:236797/149‑1 (MQ=255)
                                          ccTTGACATGGTGGTGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGaa                                                                                                          <  6:23816/149‑1 (MQ=255)
                                           cTTGACATGGTGGGGGTGGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGAGGCAAAGCGCTTTCATTCGaaa                                                                                                         <  6:366343/149‑1 (MQ=255)
                                                cATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGtttt                                                                                                    >  3:394582/1‑149 (MQ=255)
                                                 aTGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGttttt                                                                                                   <  4:266313/149‑1 (MQ=255)
                                                          gTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTtct                                                                                          <  8:393660/149‑1 (MQ=255)
                                                                 gTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCaaaaaa                                                                                   <  2:426744/149‑1 (MQ=255)
                                                                  ttCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTATCaaaa                                                                                     >  3:121868/1‑146 (MQ=255)
                                                                     gAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGc                                                                               <  3:61832/149‑1 (MQ=255)
                                                                                tgCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTAc                                                                    >  6:44367/1‑149 (MQ=255)
                                                                                tgCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTAc                                                                    <  2:345934/149‑1 (MQ=255)
                                                                                  ccTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACg                                                                   >  8:349842/1‑148 (MQ=255)
                                                                                   cTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCa                                                                 <  8:118483/149‑1 (MQ=255)
                                                                                       cAAATCGAAAAAGGGGTGATCCGAAAGGGTCCCCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCgg                                                             >  5:232582/1‑149 (MQ=255)
                                                                                       cAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCgg                                                             <  5:379206/149‑1 (MQ=255)
                                                                                       cAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCgg                                                             <  5:372343/149‑1 (MQ=255)
                                                                                               aaaaGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGCAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTa                                                      >  2:81554/1‑148 (MQ=255)
                                                                                                 aaGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAata                                                   <  1:257706/149‑1 (MQ=255)
                                                                                                      gTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAg                                                                        <  6:300959/123‑1 (MQ=255)
                                                                                                      gTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGAGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAg                                                                        >  5:300959/1‑123 (MQ=255)
                                                                                                             gAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTTATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCacac                                       <  5:442170/149‑1 (MQ=255)
                                                                                                                  ggTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAg                                  >  3:361409/1‑149 (MQ=255)
                                                                                                                   gTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAgg                                 >  6:202689/1‑149 (MQ=255)
                                                                                                                       ccccTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACGGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGcaca                             >  6:77885/1‑149 (MQ=255)
                                                                                                                       ccccTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGcaca                             <  7:12289/149‑1 (MQ=255)
                                                                                                                          cTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAg                          <  5:44367/149‑1 (MQ=255)
                                                                                                                                tCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGt                    >  6:147925/1‑149 (MQ=255)
                                                                                                                                  aTTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTg                  >  1:133354/1‑149 (MQ=255)
                                                                                                                                   ttGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGGCGATAGATCAGTTgt                 >  6:302698/1‑148 (MQ=255)
                                                                                                                                      tCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTa              <  7:349842/149‑1 (MQ=255)
                                                                                                                                       gtaTTCCCGCTCGCGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGt                >  8:391227/3‑146 (MQ=255)
                                                                                                                                       gtaTTCCCGCTCGCGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTag             >  6:277981/3‑149 (MQ=255)
                                                                                                                                       ccATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGACACTAATATGCGCAACACACGACAGTCACAAAGATAAGTTgtt                >  5:111693/1‑144 (MQ=255)
                                                                                                                                         aTTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCCCATAGCTCAGTTGGTTagag           >  6:168461/1‑149 (MQ=255)
                                                                                                                                         aTTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTagag           >  4:274564/1‑149 (MQ=255)
                                                                                                                                          ttGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGCGCTTTACAGGTACGCATCGGGCCACTAATATGCGCAGCACACGACAGGCACATAGCTCAGTTGGTTagag           >  5:338078/1‑148 (MQ=255)
                                                                                                                                                   gAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATgc                                                <  4:344245/102‑1 (MQ=255)
                                                                                                                                                   gAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCtt  >  3:192332/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTT  >  NC_002947/2796479‑2796773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑