Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,865,333 G→C 8.6% A17G (GCC→GGC)  gllP ← porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,865,3330GC 8.6% 176.9 / 7.4 60A17G (GCC→GGC) gllPporin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (2/3);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

GAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTGGGGGTGGCGGCGC  >  NC_002947/2865188‑2865476
                                                                                                                                                 |                                                                                                                                               
gAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGgctggctg                                                                                                                                              >  3:339471/1‑149 (MQ=255)
   gATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGTCAGGGCTTGCAGTGGCAGGCTGGCTGCCa                                                                                                                                           <  7:16911/149‑1 (MQ=255)
     tGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGt                                                                                                                                         >  7:69002/1‑149 (MQ=255)
     tGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGCCAGCCTGCCact                                                                                                                                         <  8:394926/149‑3 (MQ=255)
       aaaCTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGc                                                                                                                                       >  7:106283/1‑149 (MQ=255)
       aaaCTTTGCGTCCATTCCTCGGCATTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTGCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGc                                                                                                                                       <  3:279864/149‑1 (MQ=255)
        aaCTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCACTGGCAGCCAGCCTGCCACTGc                                                                                                                                       <  6:142615/148‑1 (MQ=255)
         aCTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGa                                                                                                                                     <  8:69002/149‑1 (MQ=255)
           tttGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAAt                                                                                                                                   >  3:421126/1‑149 (MQ=255)
             tGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAAt                                                                                                                                   <  5:284607/147‑1 (MQ=255)
                    aTTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACg                                                                                                                          >  3:23090/1‑149 (MQ=255)
                       ccTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAg                                                                                                                        <  2:292840/148‑1 (MQ=255)
                        cTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCg                                                                                                                      >  6:139093/1‑149 (MQ=255)
                            gCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAAt                                                                                                                                   <  3:115889/132‑1 (MQ=255)
                            gCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAAt                                                                                                                                   >  4:115889/1‑132 (MQ=255)
                                        cTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCa                                                                                                      >  2:61059/1‑149 (MQ=255)
                                            ccGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCAttt                                                                                                   <  2:418601/148‑1 (MQ=255)
                                               tGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGTCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCatt                                                                                               <  4:421126/149‑1 (MQ=255)
                                                gCCCGTGTAGTCGCGGTTCAGGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCatta                                                                                              >  3:223220/1‑149 (MQ=255)
                                                  ccGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCattatt                                                                                            >  3:106724/1‑149 (MQ=255)
                                                    gtgtAGTCGCGGTGCATGTAGAAGCTACGCAACGTCAGGCGTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGGGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTAtttt                                                                                          <  5:397831/149‑1 (MQ=255)
                                                      gtAGTCGCGGTTCATGTAGAAGGTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTa                                                                                        <  6:330265/149‑1 (MQ=255)
                                                           cgcgGTTGATGTCTAAGTTAGGGAACGTCAGGGTTGCATTGGCATCGTCGACGAAGCGTTCGGCGAGTGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGATCGGGTACAGGGGGCGGCATTTCATTATTTTTATtct                                                                                    <  5:281088/148‑1 (MQ=255)
                                                             cgGTTCATGTATAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCa                                                                                 <  7:360815/149‑1 (MQ=255)
                                                             cgGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCa                                                                                 <  4:23090/149‑1 (MQ=255)
                                                                   aTGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGAt                                                                            <  4:339471/148‑1 (MQ=255)
                                                                     gTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGTTACAGGGTGCGGCATTTCATTAttttt                                                                                         <  3:295624/133‑1 (MQ=255)
                                                                     gTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGTTACAGGGTGCGGCATTTCATTAttttt                                                                                         >  4:295624/1‑133 (MQ=255)
                                                                      tAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCa                                                                                 <  6:91519/140‑1 (MQ=255)
                                                                      tAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCa                                                                                 >  5:91519/1‑140 (MQ=255)
                                                                      tAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCATGGATggg                                                                         <  4:166240/148‑1 (MQ=255)
                                                                        gAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCa                                                                       >  6:235428/1‑148 (MQ=255)
                                                                                      tCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGc                                                          >  6:89701/1‑147 (MQ=255)
                                                                                          gCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGACAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAgcgg                                                    >  7:227644/1‑149 (MQ=255)
                                                                                          gCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAAGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAgcgg                                                    >  4:194732/1‑149 (MQ=255)
                                                                                           cTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAgcggc                                                   >  5:270633/1‑149 (MQ=255)
                                                                                                 ttGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGACTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCg                                             >  7:129911/1‑149 (MQ=255)
                                                                                                    gCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGgc                                           <  4:223220/148‑1 (MQ=255)
                                                                                                       tcgtcgACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAAGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTg                                       <  3:194732/149‑1 (MQ=255)
                                                                                                          tcgACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTggg                                     >  7:335759/1‑148 (MQ=255)
                                                                                                          tcgACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTggg                                     >  8:11346/1‑148 (MQ=255)
                                                                                                          tcgACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGc                                    <  8:335759/149‑1 (MQ=255)
                                                                                                           cgacgaAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTggg                                     >  8:315327/1‑147 (MQ=255)
                                                                                                             acgaAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAgg                                 <  7:2117/149‑1 (MQ=255)
                                                                                                               gaAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGtt                               <  1:61059/149‑1 (MQ=255)
                                                                                                                 aGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGa                             <  5:235428/149‑1 (MQ=255)
                                                                                                                  gCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGaa                            <  6:270633/149‑1 (MQ=255)
                                                                                                                  gCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGaa                            <  8:106283/149‑1 (MQ=255)
                                                                                                                   gcTTCGCCCAGGGCTTGCAGTGGCAGGCTAGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATg                                                                           <  6:420138/100‑1 (MQ=255)
                                                                                                                    cTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTgg                                      <  6:158075/137‑1 (MQ=255)
                                                                                                                    cTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTgg                                      >  5:158075/1‑137 (MQ=255)
                                                                                                                     ttCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAAt                           >  1:334534/1‑147 (MQ=255)
                                                                                                                     ttCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACAAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAAt                           >  1:6123/1‑147 (MQ=255)
                                                                                                                        ggCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAgg                       <  5:139093/148‑1 (MQ=255)
                                                                                                                           cAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGc                   >  5:49220/1‑149 (MQ=255)
                                                                                                                               gCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTGATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTg               >  3:235906/1‑149 (MQ=255)
                                                                                                                               attcgCACTGGCAGCCAGCCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTg               >  5:295106/5‑149 (MQ=255)
                                                                                                                               attcgCACTGGCAGCCAGCCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTg               <  6:295106/145‑1 (MQ=255)
                                                                                                                                    ctgTGGCAGGGTGAGTGCCAGTGCTAATGGGGGGACGAGGGGGTACAGGGTGCGGCTTTTAATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTTAATCAGGGGGCGGTGGGGGt          <  8:129911/147‑1 (MQ=255)
                                                                                                                                    cactGGCAGCCAGCCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTGGGGGt          >  3:31909/4‑149 (MQ=255)
                                                                                                                                         gCAGGCTGGCTGCCAGTGCGAATGTGGGTACGAGCGCGTACAGGGTGCGTCATTTCATAATTTTTAATCTACAGGGATAGGCAAAGCGCTGCCCGCAAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTGGGGGTggcgg     >  5:310153/1‑149 (MQ=255)
                                                                                                                                             gctggctgCCTGTGCGATGGTGGGTCCGCGGGGGTACTGGTTGCGCCATTTCATTTTTTTTTTTCTCCTGGGATGGGCAACGCGGTGCCCGCCAGCGGGGCATGGGCGCTGGGCTGGTTTAATCAGGGGGCGGTGGGGGTGGCGgcgc  <  4:106724/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGGCTTGCCTTGGCATCGTCGACGAAGCCTTCGGCCAGGGCTTGCAGTGGCAGGCTGGCTGCCAGTGCGAATGTGGGGACGAGCGGGTACAGGGTGCGGCATTTCATTATTTTTATTCTCCAGGGATGGGCAAAGCGCTGCCCGCCAGCGGCGCATCGGCGCTGGGCAGGTTGAATCAGGGGGCGGTGGGGGTGGCGGCGC  >  NC_002947/2865188‑2865476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑