| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 274,145 | A→T | 5.9% | L142Q (CTG→CAG) | PP_0221 ← | methionine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 274,145 | 0 | A | T | 5.9% | 208.3 / 2.7 | 68 | L142Q (CTG→CAG) | PP_0221 | methionine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (30/33); new base T (2/2); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
CACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCC > NC_002947/274000‑274293 | cACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagca < 4:194925/149‑1 (MQ=255)cACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagca < 4:201672/149‑1 (MQ=255)cACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagc < 4:311486/148‑1 (MQ=255) ggTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCTCCTCGACCTGAAGGATCTTAAGCTTTTTAGGGTTGGCGAGTATGTCAGCCTAGGTGTACTTGTAGCACACGCCGGGATTGATGG‑TGATCAAC‑CCGGCCTTGGCcagcagcag > 8:203036/1‑148 (MQ=255) tCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagcagcagcag < 3:68953/149‑1 (MQ=255) ggCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGc > 8:329053/1‑149 (MQ=255) gtcgtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGATGTATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCaa < 2:314053/149‑2 (MQ=255) gtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagcagca < 3:219749/137‑1 (MQ=255) gtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCcagcagca > 4:219749/1‑137 (MQ=255) gtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGc > 4:413188/1‑149 (MQ=255) ggCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAACAGCGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCggg < 6:182733/148‑3 (MQ=37) ggCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑t > 2:270691/1‑149 (MQ=255) gcgcGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑tt > 3:267145/1‑149 (MQ=255) tACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAACAGCGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCG‑GG‑TTGATca < 8:242476/149‑2 (MQ=25) ccAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCggg > 2:270644/1‑149 (MQ=255) cACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTgg < 3:77184/149‑1 (MQ=255) cACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCCGGTCGTTgg > 7:228909/1‑149 (MQ=255) gCCTCGACCTGAAGGATCATCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGAt < 5:12758/149‑1 (MQ=255) tCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGc > 5:241528/1‑149 (MQ=255) cGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGc > 2:25797/1‑148 (MQ=255) gACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGc < 1:270691/147‑1 (MQ=255) ccgAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATc < 1:332645/125‑1 (MQ=255) ccgAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATc > 2:332645/3‑127 (MQ=255) aaGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTgg > 7:391253/1‑149 (MQ=255) aaGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTgg > 1:48334/1‑149 (MQ=255) aGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGgc < 2:135035/149‑1 (MQ=255) tCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCt > 6:139130/1‑149 (MQ=255) ttCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCAGGGCTTGAGGG‑TGATCAAC‑ACGGCCTTGGCCAGCATCAGCAGGCCACGGCAGATG‑TTGATCTGGTCGTTGGCGATGGCCACCCTGGCGCCtt > 5:338347/1‑148 (MQ=255) tCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCtt > 4:240069/1‑147 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGTGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGTCGCCtt < 7:203036/146‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGATTAAAAAA‑CCCGCCTTTGGCAAGAGGAGCCCGCCACGGGCGGGGTTTGAACGGTTCTTTGGCGATGGCCACGATgccccat > 1:131063/1‑141 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGcc < 6:199104/144‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGcc < 4:267145/144‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCtt < 4:205913/146‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCt < 7:163102/145‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTc < 3:426178/147‑1 (MQ=255) cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCa < 3:240069/148‑1 (MQ=255) aGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAAGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAg < 2:423271/148‑1 (MQ=255) gCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCATTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAA‑CCGGCCTTGGCCAGCAGAAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAgg > 7:293551/1‑148 (MQ=255) gggTTGGCGAGGATGTCAGCCTCGGTGGGCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTCGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCgg < 7:80512/148‑1 (MQ=255) ggTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGc > 2:348727/1‑148 (MQ=255) ttGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCa > 4:62431/1‑147 (MQ=255) aGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCtttt < 5:127518/149‑1 (MQ=255) ggATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTg < 3:399424/149‑1 (MQ=255) tggcAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTAc < 3:246977/146‑1 (MQ=255) aGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACtttt < 7:329053/149‑1 (MQ=255) ccTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTc > 7:290557/1‑149 (MQ=255) gTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTa > 6:249400/1‑149 (MQ=255) tGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTa > 6:151034/1‑148 (MQ=255) tGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTa > 7:212331/1‑148 (MQ=255) ggCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTa > 5:192648/1‑147 (MQ=255) gCCTTGTAGCCGACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAg < 5:160233/149‑1 (MQ=255) gCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCCGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAg > 6:266241/1‑149 (MQ=255) ccTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAgg > 5:178949/1‑149 (MQ=255) cTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGc < 3:413188/149‑1 (MQ=255) gTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGcc < 5:247121/147‑1 (MQ=255) ccACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACgttg > 7:29753/1‑149 (MQ=255) gaCGCCTGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCATCAGGCCACGGACGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACgttgt < 8:293551/148‑1 (MQ=255) ccGGGCTTGAGGG‑GGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgat < 6:241528/149‑1 (MQ=255) cGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgatg < 4:9791/149‑1 (MQ=255) gCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGatgatg > 2:43160/1‑149 (MQ=255) ggg‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCggg < 6:192648/149‑1 (MQ=255) g‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCTCA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCggg < 1:343246/149‑1 (MQ=255) aTCAAC‑CCGGGCTTTGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTTATGATGCCCGGGGCGt < 6:338347/148‑1 (MQ=255) tCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGt > 8:228068/1‑147 (MQ=255) aaC‑CCGGCCTTGGCCAGCCGCAGCAGGCCACGGCCGCTT‑GTGATCGGGTCGTTGGCGTTGGCCACGCTGGCGCCTTCAGG‑‑CACTGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTaa < 1:348727/149‑1 (MQ=255) c‑ccGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGca > 3:37774/1‑149 (MQ=255) gcGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCG‑GG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGcac > 8:343721/2‑148 (MQ=38) cGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGcaca < 6:178949/149‑1 (MQ=255) cGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGcac > 1:212053/1‑148 (MQ=255) cgtGGCCTGCTGCTGCTGGCCAAGGCCG‑GG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGtt > 7:288013/3‑149 (MQ=38) ggCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTg < 7:228068/148‑1 (MQ=255) gCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGaa < 8:29753/149‑1 (MQ=255) ccAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAgg < 2:128553/136‑1 (MQ=255) ccAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAgg > 1:128553/1‑136 (MQ=255) cagcagcagcagGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGc < 8:212331/149‑1 (MQ=255) cagcagcagcagGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGc > 8:53940/1‑149 (MQ=255) agcagcagcagGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGcc < 2:131063/149‑1 (MQ=255) | CACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGG‑TGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTG‑TTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGG‑‑CA‑‑GCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCC > NC_002947/274000‑274293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |