Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,946,588 G→C 9.0% P312A (CCG→GCG) ‡ yijP ← phosphoethanolamine transferase CptA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,946,5880GC 9.0% 195.3 / 7.8 64P312A (CCG→GCG) ‡yijPphosphoethanolamine transferase CptA
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base C (4/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCGTG  >  NC_002947/2946446‑2946736
                                                                                                                                              |                                                                                                                                                    
ttGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCt                                                                                                                                                >  1:57404/1‑149 (MQ=255)
   cgcTGGGTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGat                                                                                                                                             <  4:106209/149‑2 (MQ=255)
        gttgttCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGaaa                                                                                                                                         <  1:423153/148‑1 (MQ=255)
        gttgttCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGaaa                                                                                                                                         <  1:429841/148‑1 (MQ=255)
        gttgttCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGaaa                                                                                                                                         <  2:316791/148‑1 (MQ=255)
            ttCAGGTACACCGGTGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGt                                                                                                                                     <  1:161799/148‑1 (MQ=255)
            ttCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGt                                                                                                                                     <  4:203466/148‑1 (MQ=255)
             tCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTcc                                                                                                                                   >  8:335167/1‑149 (MQ=255)
               aGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCTTGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCgg                                                                                                                                 <  4:324227/149‑1 (MQ=255)
                     acCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTg                                                                                                                           <  1:318609/149‑1 (MQ=255)
                           gcgTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCtcgtc                                                                                                                     <  6:163875/149‑1 (MQ=255)
                             ggatGTTTGCTGCGAGACGGTGGTGAGCAGTGTGTTGCGCTTGGGCCAGGGCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGc                                                                                                                   <  4:247000/146‑1 (MQ=255)
                                  tttGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAAc                                                                                                              >  8:314591/1‑149 (MQ=255)
                                         cGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGa                                                                                                       >  2:213218/1‑149 (MQ=255)
                                           agaAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATc                                                                                                     >  4:205244/1‑149 (MQ=255)
                                           agaAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATc                                                                                                     <  7:314591/149‑1 (MQ=255)
                                           agaAGGTGGTGAGCATGGTGCTGCGCTTGGTCAAGGTCGGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATc                                                                                                     <  4:291479/149‑1 (MQ=255)
                                             aaGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATctg                                                                                                   >  3:380877/1‑149 (MQ=255)
                                                   gtgAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCAt                                                                                             >  3:269564/1‑149 (MQ=255)
                                                      aGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCAc                                                                                          <  3:205244/149‑1 (MQ=255)
                                                        cATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACtt                                                                                        <  7:335167/149‑1 (MQ=255)
                                                              gtTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATgg                                                                                  >  8:22165/1‑149 (MQ=255)
                                                               ttacgcTTGATCAAGCTCTGCTCGTTGGAGATCCACAAGGTCTTGTAGCCTGCCTGTTTCATAAGGTTGATCAGCGACCCGTCGCTGAGCAAACGGTCCGGGTTCTCCTCGTCGCCGACCCTCAGGAACTGCTGCATCACTACGAtctt                                                                                 >  8:95417/4‑146 (MQ=255)
                                                                       ggTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCCCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGgc                                                                         >  8:218569/1‑149 (MQ=255)
                                                                              gTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGa                                                                  >  3:374665/1‑149 (MQ=255)
                                                                              gTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCCGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGa                                                                  >  5:163986/1‑149 (MQ=255)
                                                                                    tggttggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCAc                                                            >  1:341178/1‑149 (MQ=255)
                                                                                      gttggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGt                                                          >  3:211991/1‑149 (MQ=255)
                                                                                       ttggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAgg                                                                                                        <  3:402874/102‑1 (MQ=255)
                                                                                       ttggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAgg                                                                                                        >  4:402874/1‑102 (MQ=255)
                                                                                         ggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcg                                                        <  2:57404/148‑1 (MQ=255)
                                                                                         ggtGATCCAGAAGGTCTTGTAGCCAGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTAGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgc                                                       >  2:181533/1‑149 (MQ=255)
                                                                                          gtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgcg                                                      <  7:22165/149‑1 (MQ=255)
                                                                                           tGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgcg                                                      >  4:309294/1‑148 (MQ=255)
                                                                                                  gAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAAc                                                                                                              >  6:363768/1‑85 (MQ=255)
                                                                                                  gAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAAc                                                                                                              <  5:363768/85‑1 (MQ=255)
                                                                                                  gAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACgg                                              <  1:67281/149‑1 (MQ=255)
                                                                                                  gAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACgg                                              >  7:211919/1‑149 (MQ=255)
                                                                                                    aGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTc                                            <  8:279900/149‑1 (MQ=255)
                                                                                                       tCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAgg                                         <  4:269564/149‑1 (MQ=255)
                                                                                                         ttGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGc                                        >  2:177436/1‑148 (MQ=255)
                                                                                                           gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCTTCGCCGAACGTGAGGATCTGCTTCATCACTTCGATGGTGTATGGTCTCGGCGATACCACGTCGCGGAACAGTGTCAGtctc                                      <  4:380877/148‑1 (MQ=255)
                                                                                                           gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGctct                                     >  7:411603/1‑149 (MQ=255)
                                                                                                           gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGctc                                      <  4:374665/148‑1 (MQ=255)
                                                                                                           gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGc                                        <  1:213218/146‑1 (MQ=255)
                                                                                                            tAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGctct                                     <  2:341178/148‑1 (MQ=255)
                                                                                                                ctgcctgTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCg                                >  8:369604/1‑149 (MQ=255)
                                                                                                                   cctgTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCt                              <  8:411603/148‑1 (MQ=255)
                                                                                                                   cctgTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGACGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTg                             >  5:220880/1‑149 (MQ=255)
                                                                                                                    ctgTTTCAGCAGGTTGATCAGCGACGGTTCGCTGAGGAAACGGTCCGGGTGCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTAGGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTgg                            <  7:95417/149‑1 (MQ=255)
                                                                                                                       tttCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTggcgg                         >  8:391757/1‑149 (MQ=255)
                                                                                                                           agcagGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGcc                       <  6:351578/147‑1 (MQ=255)
                                                                                                                           agcagGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGcc                       >  5:351578/1‑147 (MQ=255)
                                                                                                                            gcagGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAgc                    <  8:211919/149‑1 (MQ=255)
                                                                                                                            gcagGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATAACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGAGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAgc                    >  5:72391/1‑149 (MQ=255)
                                                                                                                               ggTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGACCACGGTCAGGCTCTTGTCGCTGGCGGCCAGcgcg                 >  4:298680/1‑149 (MQ=255)
                                                                                                                                gTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGCACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGt                >  2:117206/1‑149 (MQ=255)
                                                                                                                                    ctCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAg            >  8:167735/2‑149 (MQ=255)
                                                                                                                                    ctCAGCGACCCGTCGCTGAGGAAACGGTCCGGGGTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGt                                             <  4:60122/115‑1 (MQ=255)
                                                                                                                                    ctCAGCGACCCGTCGCTGAGGAAACGGACCGGGTTCTGCTCGTCGCCGACCGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACgct                                             >  3:60122/2‑114 (MQ=255)
                                                                                                                                    aTCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTAGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGTCGCGGCGATACCACGTCCCGGATCACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGGCGac            >  8:390366/1‑148 (MQ=255)
                                                                                                                                     tCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAgg           >  7:61148/1‑149 (MQ=255)
                                                                                                                                        gCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTg        >  8:54124/1‑149 (MQ=255)
                                                                                                                                        gCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCCCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTg        <  7:218569/149‑1 (MQ=255)
                                                                                                                                              ggTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCgtg  <  1:181533/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCGTG  >  NC_002947/2946446‑2946736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑