Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,989,215 G→T 7.3% R43R (CGG→AGG)  PP_2613 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,989,2150GT 7.3% 166.6 / 3.8 55R43R (CGG→AGG) PP_2613hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

CCAGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCAT  >  NC_002947/2989068‑2989363
                                                                                                                                                   |                                                                                                                                                    
ccaTCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGa                                                                                                                                                     <  3:51313/149‑1 (MQ=255)
          cccAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCTATCGGCCCatc                                                                                                                                           <  7:31683/149‑3 (MQ=255)
          cccAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGt                                                                                                                                           >  5:289418/1‑149 (MQ=255)
           ccAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGtt                                                                                                                                          >  2:273964/1‑149 (MQ=255)
            cAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTtc                                                                                                                                         >  5:140799/1‑149 (MQ=255)
            cAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTtc                                                                                                                                         >  4:193191/1‑149 (MQ=255)
             aaaCTCATGACGGTCATCCAGGGCTCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTtct                                                                                                                                        <  1:235016/149‑1 (MQ=255)
             aaaCTCATGACGGTCATCCAGGGAGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGAAAGAGTTTATAAGGTCTAGTAAAGATGGGGCATAAGAAGGAAACAGTTAACCTTCATGCGGGATGGGACGATAGGCACAGtta                                                                                                                                         >  4:6314/1‑147 (MQ=255)
             aaaCACATGACGGACATCCAGGGAGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTAGGCTCACAGCGTTTATAAGGTAGAGTACAGAAGGGGCATAAGCACGAACCACTTCACCAAACTGCGGCAAGGACCGAAAGGCCCACttat                                                                                                                                        >  4:347542/1‑147 (MQ=255)
              aaCTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTtctc                                                                                                                                       <  8:250211/149‑1 (MQ=255)
                cTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGa                                                                                                                                     <  5:371085/149‑1 (MQ=255)
                    tttcGGGCATCCAGGGCGGTGTTGTTGGATAACCATGCGTTTTCATTATTTCGGCTGACATCGTTTATAATGTCGAGTACGTATGGGTCATAAGCATGATCCAGTTCACGTTCTTGCGGGATGGGCCGATAGGCCCAGTTCTCGAttt                                                                                                                                  <  3:347542/145‑2 (MQ=255)
                     gACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGAATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGc                                                                                                                                 >  5:293209/1‑148 (MQ=255)
                        ggTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTg                                                                                                                             <  5:314079/149‑1 (MQ=255)
                          tCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGt                                                                                                                           <  3:193191/149‑1 (MQ=255)
                           cATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGt                                                                                                                           >  7:193962/1‑148 (MQ=255)
                              ccAGAGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCAt                                                                                                                        >  8:178271/1‑148 (MQ=255)
                                  ggCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGagca                                                                                                                   <  6:289418/149‑1 (MQ=255)
                                   gcgcTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGagcag                                                                                                                  <  4:281488/149‑1 (MQ=255)
                                     gcTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTCCCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAgcagca                                                                                                                <  4:237455/149‑1 (MQ=255)
                                     gcTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAgcagca                                                                                                                <  8:44867/149‑1 (MQ=255)
                                             tcCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCgg                                                                                                        >  7:412374/1‑149 (MQ=255)
                                              ccATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCgg                                                                                                        <  2:238207/148‑1 (MQ=255)
                                               cATAACCATGCCTTTTGAGTATTTCGGCTGACTGCTTTTATTAGGTCGTGTACCGATGGTGCATAAGCAGGAACCAGTTCACCTTCGTGCGTGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGag                                                                                                                     <  8:182325/134‑1 (MQ=255)
                                                   aCCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTtg                                                                                                  <  6:140799/149‑1 (MQ=255)
                                                      ttGCCTTTTGAGTATTTCGGATGACAGCGTTTATAAGGTCGAGTACCGATTGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGTATGGGCCGATATGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGa                                                                                               <  3:6314/148‑1 (MQ=255)
                                                          cTTTTGAGTATTTCGGCTGACAGCTTTTATTAGGTCTAGTACCGATGGGGGATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGa                                                                                           <  8:412374/149‑1 (MQ=255)
                                                            tttGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAg                                                                                         >  6:230806/1‑149 (MQ=255)
                                                                              cAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCggagga                                                                       <  1:273964/149‑1 (MQ=255)
                                                                                gCGTTTATAAGGTCGAGTACCGATGGGGTATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGaggagg                                                                     >  4:237039/1‑149 (MQ=255)
                                                                                  gtctataCGGTCGAGTCCCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACT‑GTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGtga                                                                  <  4:246192/146‑1 (MQ=255)
                                                                                     tataAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGtgatg                                                                >  2:115190/1‑149 (MQ=255)
                                                                                     tataAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGtgat                                                                 <  3:380269/148‑1 (MQ=255)
                                                                                     tataAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCACGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGtgat                                                                 <  8:193962/148‑1 (MQ=255)
                                                                                     tataAGGTCGAGTACCGATGGGGAATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGtgat                                                                 <  6:293209/148‑1 (MQ=255)
                                                                                          ggTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAg                                                           >  1:277363/1‑149 (MQ=255)
                                                                                              gAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCa                                                                                                      <  5:240324/102‑1 (MQ=255)
                                                                                              gAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCa                                                                                                      >  6:240324/1‑102 (MQ=255)
                                                                                              gAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAATTGTGAGCGAAGTCTTCGCTGCGCggag                                                                         >  5:245387/1‑131 (MQ=255)
                                                                                              gAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAATTGTGAGCGAAGTCTTCGCTGCGCggag                                                                         <  6:245387/131‑1 (MQ=255)
                                                                                                     gATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTgg                                                <  5:230806/149‑1 (MQ=255)
                                                                                                           gCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACtt                                            <  7:125509/147‑1 (MQ=255)
                                                                                                           gCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACtt                                            >  8:125509/1‑147 (MQ=255)
                                                                                                            cATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGcc                                         >  7:220698/1‑149 (MQ=255)
                                                                                                             aTAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGGGATGACAAGGTGGTCATTGGACTTGccc                                        >  3:284468/1‑149 (MQ=255)
                                                                                                                 gCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCg                                     >  2:107213/1‑148 (MQ=255)
                                                                                                                              cACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAAt                       >  2:229424/1‑149 (MQ=255)
                                                                                                                                  ttCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggtg                                                        <  8:176286/112‑1 (MQ=255)
                                                                                                                                  ttCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggtg                                                        >  7:176286/1‑112 (MQ=255)
                                                                                                                                      tGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAgg               >  6:275081/1‑149 (MQ=255)
                                                                                                                                        cGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAgg               <  2:277363/147‑1 (MQ=255)
                                                                                                                                        agaactGGGCCTATCGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAggg              >  7:100103/6‑148 (MQ=255)
                                                                                                                                          aactGGGCCTATCGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAgg               <  2:56608/142‑1 (MQ=255)
                                                                                                                                          aactGGGCCTATCGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAgg               >  1:56608/4‑145 (MQ=255)
                                                                                                                                               ggCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGa       <  3:237039/148‑1 (MQ=255)
                                                                                                                                                   gATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCAt  >  7:261826/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGATAGGCCCAGTTCTCGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCAT  >  NC_002947/2989068‑2989363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑