Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,008,313 A→G 7.7% M261T (ATG→ACG)  PP_2628 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,008,3130AG 7.7% 142.9 / 2.2 52M261T (ATG→ACG) PP_2628ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

TTCGAACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGA  >  NC_002947/3008165‑3008460
                                                                                                                                                     |                                                                                                                                                    
ttCGAACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGGCa                                                                                                                                                      >  8:162158/1‑149 (MQ=255)
  cGAACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCAt                                                                                                                                                     <  7:162158/148‑1 (MQ=255)
  cGAACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATc                                                                                                                                                    >  2:198195/1‑149 (MQ=255)
           tCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCgg                                                                                                                                           >  1:402738/1‑149 (MQ=255)
             aCCAGGCGCATCTGGCGAAATCCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCggg                                                                                                                                          >  5:170041/1‑148 (MQ=255)
                aGGCGCCTCTGGCGAAAACCGGTCTGCTTGAAATCATCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCAtt                                                                                                                                      <  6:316350/149‑1 (MQ=255)
                  gcgcATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCAGTgg                                                                                                                                    >  4:96713/1‑149 (MQ=255)
                         tGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GATc                                                                                                                            >  5:427915/1‑149 (MQ=255)
                           gCGACAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTc                                                                                                                            <  2:412049/147‑1 (MQ=255)
                           gCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCt                                                                                                                          >  3:136697/1‑149 (MQ=255)
                              aaaaCCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCggg                                                                                                                                          >  1:346562/1‑131 (MQ=255)
                              aaaaCCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCggg                                                                                                                                          <  2:346562/131‑1 (MQ=255)
                                  ccGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGc                                                                                                                        <  8:8246/144‑1 (MQ=255)
                                  ccGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGc                                                                                                                        >  7:8246/1‑144 (MQ=255)
                                   cGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGAcc                                                                                                                  <  2:233032/149‑1 (MQ=255)
                                   cGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGAc                                                                                                                   <  6:170041/148‑1 (MQ=255)
                                          cTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCAGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCa                                                                                                           >  4:158982/1‑149 (MQ=255)
                                           ttGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAg                                                                                                          >  6:18257/1‑149 (MQ=255)
                                             gAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGc                                                                                                         <  8:184951/148‑1 (MQ=255)
                                                  cTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGCGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCAGCCCGACCTTGCCCAGCCgggg                                                                                                    >  5:232700/1‑148 (MQ=255)
                                                   ttCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGa                                                                                                  >  5:228192/1‑149 (MQ=255)
                                                   ttCCATGGCTGCGCGCGCAAACTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGGGGGTATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCTAGCTCCTGCCCGACCTTGCCCAGCCggggg                                                                                                   <  1:254327/149‑1 (MQ=255)
                                                     ccATGGCTGCGTGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAACACCCGCCCGGTCATCGACGACCGGGCATTgg                                                                                                                                    <  1:45969/115‑1 (MQ=255)
                                                     ccATGGCTGCGTGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTgg                                                                                                                                    >  2:45969/1‑114 (MQ=255)
                                                           cTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATAc                                                                                          <  4:136697/149‑1 (MQ=255)
                                                            tGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATa                                                                                           >  3:42236/1‑147 (MQ=255)
                                                            tGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATa                                                                                           >  7:169228/1‑147 (MQ=255)
                                                               gcgcgcAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCa                                                                                      <  2:402738/149‑1 (MQ=255)
                                                                cgcgcAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCAt                                                                                     <  8:169228/149‑1 (MQ=255)
                                                                 gcgcAAAGTGCGCTTCGCGGGTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATg                                                                                    <  2:334616/149‑1 (MQ=255)
                                                                           cgcTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCg                                                                           >  7:320147/1‑148 (MQ=255)
                                                                            gcTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGaa                                                                         <  6:120618/149‑1 (MQ=255)
                                                                            gcTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGaa                                                                         <  5:18257/149‑1 (MQ=255)
                                                                              ttCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACa                                                                       >  8:225529/1‑149 (MQ=255)
                                                                                  cgGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCAc                                                                   >  1:192112/1‑149 (MQ=255)
                                                                                                      ttttACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAggg                                                 >  2:259436/1‑147 (MQ=255)
                                                                                                           ccGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATc                                          <  4:42236/149‑1 (MQ=255)
                                                                                                                   aTGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCa                                   <  3:158982/148‑1 (MQ=255)
                                                                                                                   aTGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCa                                   <  7:225529/148‑1 (MQ=255)
                                                                                                                    tGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTGATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAgg                                 <  6:228192/149‑1 (MQ=255)
                                                                                                                    tGTTGGGGTTAGCTTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GATCCTGCCCGACCTTGCCCAGCCGTGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAgg                                 <  6:232700/149‑1 (MQ=255)
                                                                                                                       tGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGc                               <  2:192112/148‑1 (MQ=255)
                                                                                                                         gtgtAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCaaa                            <  1:198195/149‑1 (MQ=255)
                                                                                                                          tgtAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAg                           <  6:417604/149‑1 (MQ=255)
                                                                                                                          tgtAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAg                           <  8:320147/149‑1 (MQ=255)
                                                                                                                            tAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAgcc                         >  8:10045/1‑149 (MQ=255)
                                                                                                                              aGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGTACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAgccgc                       <  1:328307/149‑1 (MQ=255)
                                                                                                                              aGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAAAAAAAGATGGCCAACTGGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAgccgc                       >  1:360018/1‑149 (MQ=255)
                                                                                                                                      ggccaatgcccgGTCGTCGATGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAgccgcc                      <  7:20965/136‑1 (MQ=255)
                                                                                                                                      ggccaatgcccgGTCGTCGATGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAgccgcc                      >  8:20965/8‑143 (MQ=255)
                                                                                                                                             gcccgGTCGTCGATGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAgg                                 <  6:420831/125‑1 (MQ=255)
                                                                                                                                             gcccgGTCGTCGATGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAgg                                 >  5:420831/1‑125 (MQ=255)
                                                                                                                                                   tCATCGACGACCGGCCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGACCAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTACAGGGCAAAGCCGCCGGGCAGGGCCATGAgcgc    >  8:307623/1‑148 (MQ=255)
                                                                                                                                                     aTCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGa  <  7:10045/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCGAACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAA‑ACCCGCCCGGTCATCGACGACCGGGCATTGGCCT‑GCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGA  >  NC_002947/3008165‑3008460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑