Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,024,211 A→T 6.2% intergenic (+26/+128) dapA‑II → / ← PP_2640 4‑hydroxy‑tetrahydrodipicolinate synthase/GNAT family acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,024,2110AT 6.2% 226.2 / 2.4 74intergenic (+26/+128)dapA‑II/PP_26404‑hydroxy‑tetrahydrodipicolinate synthase/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base T (2/3);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGCTGGCTGGGTCGGCAG  >  NC_002947/3024067‑3024359
                                                                                                                                                 |                                                                                                                                                    
tCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAg                                                                                                                                                  >  2:140810/1‑149 (MQ=255)
  aaGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgcc                                                                                                                                                >  4:194802/1‑149 (MQ=255)
  aaGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgcc                                                                                                                                                >  2:419190/1‑149 (MQ=255)
  aaGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgcc                                                                                                                                                >  8:217819/1‑149 (MQ=255)
    ggcggcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgccgc                                                                                                                                              <  8:107439/149‑1 (MQ=255)
     gcggcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgccgc                                                                                                                                              >  5:87958/1‑148 (MQ=255)
      cggcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCca                                                                                                                                            <  6:407697/149‑1 (MQ=255)
       ggcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCca                                                                                                                                            >  4:347353/1‑148 (MQ=255)
         cATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCacac                                                                                                                                         <  2:166957/149‑1 (MQ=255)
          aTTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTGTCAACCCGCcatcaa                                                                                                                                        <  1:392619/149‑5 (MQ=255)
              cAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCtt                                                                                                                                     <  1:140810/148‑1 (MQ=255)
               aGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttgg                                                                                                                                   >  7:185218/1‑149 (MQ=255)
                gCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttggt                                                                                                                                  <  3:398182/149‑1 (MQ=255)
                  tGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttggttg                                                                                                                                >  2:243933/1‑149 (MQ=255)
                    cAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttggttg                                                                                                                                <  3:194802/147‑1 (MQ=255)
                     aaaGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTggttggtt                                                                                                                             >  6:45938/1‑149 (MQ=255)
                       aGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTTGTTGGTTCg                                                                                                                           <  4:342644/149‑1 (MQ=255)
                       aGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCg                                                                                                                           <  7:217819/149‑1 (MQ=255)
                                 cAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTcggcg                                                                                                                  <  1:107957/148‑1 (MQ=255)
                                  aGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGa                                                                                                                >  6:291555/1‑149 (MQ=255)
                                       aGCTGCGCGGGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCTAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCt                                                                                                           >  5:64905/1‑149 (MQ=255)
                                              cgAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGc                                                                                                    <  1:419190/149‑1 (MQ=255)
                                                  cccATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTCGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGa                                                                                                 >  1:108719/1‑149 (MQ=255)
                                                        cAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGc                                                                                          <  1:218460/149‑1 (MQ=255)
                                                          gACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCa                                                                                        <  6:87958/149‑1 (MQ=255)
                                                          gACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTGTCAAcccgcc                                                                                                                                             >  2:428192/1‑96 (MQ=255)
                                                          gACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTGTCAAcccgcc                                                                                                                                             <  1:428192/96‑1 (MQ=255)
                                                             gTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGc                                                                                      >  5:299593/1‑148 (MQ=255)
                                                                cACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAAcg                                                                                  >  5:358905/1‑149 (MQ=255)
                                                                cACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAAc                                                                                   <  6:326562/148‑1 (MQ=255)
                                                                 aCGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAAcgc                                                                                 <  5:384257/149‑1 (MQ=255)
                                                                   gAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACgcgc                                                                               >  2:268147/1‑149 (MQ=255)
                                                                      tACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCTAGCGGATTTCTGCCAGCAACGCGCTga                                                                            <  1:74874/149‑1 (MQ=255)
                                                                        ccaccCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                                          >  2:401821/1‑149 (MQ=255)
                                                                        ccaccCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                                          <  3:353468/149‑1 (MQ=255)
                                                                         caccCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                                          >  1:112376/1‑148 (MQ=255)
                                                                          accCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                                          >  2:291072/1‑147 (MQ=255)
                                                                          accCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                                          >  4:230324/1‑147 (MQ=255)
                                                                          accCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACa                                                                        <  5:291555/149‑1 (MQ=255)
                                                                               agaCAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTa                                                                   >  4:333159/1‑149 (MQ=255)
                                                                                 aCAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTAtt                                                                 <  1:282394/149‑1 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAttt                                                                                              <  8:81540/118‑1 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAttt                                                                                              >  7:81540/1‑118 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAt                                                                                                <  5:374274/116‑1 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAt                                                                                                >  6:374274/1‑116 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTCACAAGCCGCCACACCCTTGGATGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACTCGCTGAGACAGAGTATTGc                                                               >  3:235110/1‑149 (MQ=255)
                                                                                   aaaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGCTTGAAGGCGGGATGACAAGACGCAACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTg                                                                >  1:215801/1‑148 (MQ=255)
                                                                                    aaCTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGc                                                               >  7:210904/1‑148 (MQ=255)
                                                                                        gCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAggg                                                          >  4:52130/1‑149 (MQ=255)
                                                                                        gCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAggg                                                          >  3:280608/1‑149 (MQ=255)
                                                                                         cAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGc                                                         >  6:14707/1‑149 (MQ=255)
                                                                                           aaGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCg                                                       <  3:333159/149‑1 (MQ=255)
                                                                                            aGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGc                                                      <  1:401821/149‑1 (MQ=255)
                                                                                              cGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCtt                                                    >  6:405499/1‑149 (MQ=255)
                                                                                               gTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCttt                                                   <  1:291072/149‑1 (MQ=255)
                                                                                                 gCTGT‑GTTCACTCTGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGc                                                 <  6:242296/149‑1 (MQ=255)
                                                                                                  cTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCg                                                >  8:21536/1‑149 (MQ=255)
                                                                                                     t‑gtTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGACAGGGCCGCAAAGCGGcc                                             <  8:185218/149‑1 (MQ=255)
                                                                                                     t‑gtTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGACGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCgg                                               <  1:243933/147‑1 (MQ=255)
                                                                                                          gaCTGGGCGCCTGAAGGTGCATGGGTTGATGGCGGGTTGACATGCGGCCTGATCCTTGGTTGGTTCGGGGTCGGCGGTTGCGTCGGGTGCGGTTTTGTGGGTGCTTCGCGCTGAGTCAGAGTTTTGGCAGGGCCGCATAGCGGCCCTg                                          <  4:235110/147‑1 (MQ=255)
                                                                                                          cACTCGGCTCCTGAAGCGGAAACGGTTGATGGCGGGTTGTCAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCGCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCAAAGCGGCCCTg                                          <  8:365847/148‑1 (MQ=255)
                                                                                                             tCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAttt                                                                                              <  6:145582/93‑1 (MQ=255)
                                                                                                             tCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGAttt                                                                                              >  5:145582/1‑93 (MQ=255)
                                                                                                                       aaGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGt                            <  6:358905/149‑1 (MQ=255)
                                                                                                                            gCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTc                       >  1:430752/1‑149 (MQ=255)
                                                                                                                            gCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTc                       >  3:390045/1‑149 (MQ=255)
                                                                                                                             cAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTcc                      >  2:244494/1‑149 (MQ=255)
                                                                                                                              aaCGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTcc                      >  7:144702/1‑148 (MQ=255)
                                                                                                                                     tgtGGCGGCTTGTCAACCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACgctgg              >  6:184009/3‑149 (MQ=255)
                                                                                                                                     gATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACgctg               <  4:280608/148‑1 (MQ=255)
                                                                                                                                      aTGGAGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGATGAGACAGAGTATTGCCAGGGCCGCTTTTCGGCACTGTCGAGACACCAGGTTGCTCCTACgctggc             >  2:360700/1‑149 (MQ=255)
                                                                                                                                       tGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACgctggct            <  1:244494/149‑1 (MQ=255)
                                                                                                                                              ttGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGCTGGCTGGGTCgg     <  8:210904/149‑1 (MQ=255)
                                                                                                                                                gACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTCCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGCTGGCTGGGTCGGc    <  3:230324/148‑1 (MQ=255)
                                                                                                                                                 aCAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGCTGGCTGGGTCGGCAg  <  5:45938/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGT‑GTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGCTGGCTGGGTCGGCAG  >  NC_002947/3024067‑3024359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑