| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,054,732 | C→G | 13.1% | intergenic (‑207/+101) | PP_2666 ← / ← PP_2667 | hypothetical protein/ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,054,732 | 0 | C | G | 13.1% | 97.3 / 4.2 | 37 | intergenic (‑207/+101) | PP_2666/PP_2667 | hypothetical protein/ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (12/20); new base G (2/3); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
ACGATAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGA > NC_002947/3054586‑3054870 | acgaTAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAg > 2:104493/1‑149 (MQ=255) gaTAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCg < 5:167396/149‑1 (MQ=255) gACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCATGCGTGGCGAAAAGGCCGCGCAGCCGccc > 7:206977/1‑149 (MQ=255) aGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAg > 8:365296/1‑149 (MQ=255) gTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCTGCGCGGccctttc < 8:169622/149‑5 (MQ=255) gTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCTGCGCGGccctttc < 8:163855/149‑5 (MQ=255) gTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCTGCGCGGccctttc < 3:183191/149‑5 (MQ=255) gCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCg > 5:126520/1‑149 (MQ=255) cAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCg < 1:104493/148‑1 (MQ=255) aGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCGACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTGGCGAAAGGGCCGCGCAGCCGCCCCAGGAtgtggg > 5:368240/1‑143 (MQ=255) tagtagATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATa < 4:181016/149‑1 (MQ=255) aaGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGc < 3:88889/148‑1 (MQ=255) aTGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGCGTGCGTGGCGAAAGGGCCGCGGCCCGGCCCCCGGAGCTGGGGCGCAAGCAATGGTTGCGGGGGGCGCGTTGGGGgcc > 2:338102/1‑148 (MQ=12) aTGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCT‑GGGCCGCAAAGCGGcccca < 7:365296/149‑3 (MQ=37) gagaCGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAAt < 6:126520/148‑1 (MQ=255) gCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCgg > 1:301574/1‑137 (MQ=255) gCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCgg < 2:301574/137‑1 (MQ=255) tCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCcac < 4:243278/148‑1 (MQ=255) tcTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACgg < 8:218450/147‑1 (MQ=255) gCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACgg < 1:338102/148‑1 (MQ=255) ttaaaGCTGGCGCTATCCCTGTGGGAGCGTCATTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCa < 7:304315/147‑1 (MQ=255) gcgcGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTc < 8:206977/147‑1 (MQ=255) cgATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAgcgc < 6:170590/149‑1 (MQ=255) ctcTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGc < 1:374396/147‑1 (MQ=255) ccTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCa < 4:118940/149‑1 (MQ=255) cTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCAt < 2:230271/149‑1 (MQ=255) gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCt > 8:357590/1‑149 (MQ=255) gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAg < 5:383138/136‑1 (MQ=255) gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAg > 6:383138/1‑136 (MQ=255) cgcgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggcg > 4:5370/1‑147 (MQ=255) cgcgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCAACATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgggg > 6:253747/1‑149 (MQ=255) cctggggCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGtttt > 3:6143/5‑114 (MQ=255) cctggggCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGtttt < 4:6143/110‑1 (MQ=255) tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCAAGGTTTTCACCGCGGCTTGCGAAGCGCTGCATGCAGGCGGATg > 4:21715/3‑148 (MQ=37) aaGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCACGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGa < 6:368240/149‑1 (MQ=255) ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCg < 1:360170/79‑1 (MQ=255) ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCg > 2:360170/1‑79 (MQ=255) | ACGATAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGA > NC_002947/3054586‑3054870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |