Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,798 A→T 6.1% Y258* (TAT→TAA)  PP_5538 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,7980AT 6.1% 201.1 / 2.9 65Y258* (TAT→TAA) PP_5538ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base T (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AGCCATCCAGGCGGCGAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTT  >  NC_002947/3057651‑3057941
                                                                                                                                                      |                                                                                                                                               
aGCCATCCAGGCGGCGAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGAt                                                                                                                                                <  8:337967/149‑1 (MQ=255)
    aTCCAGGCGGCGAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCa                                                                                                                                            >  7:34233/1‑149 (MQ=255)
          gcggcgAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTg                                                                                                                                      <  8:95817/149‑1 (MQ=255)
           cggcgAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTgg                                                                                                                                     <  7:239967/149‑1 (MQ=255)
            ggcgAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTgg                                                                                                                                     >  8:137443/1‑148 (MQ=255)
             tcgAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTa                                                                                                                                   <  5:312145/148‑1 (MQ=255)
             gcgAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTa                                                                                                                                   <  4:185310/149‑1 (MQ=255)
               gAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAgg                                                                                                                                 >  1:9413/1‑149 (MQ=255)
               gAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGACCCTGGGTACCGGCTACCAGACGTGGTATCCACGTCCGGtggc                                                                                                                                 <  5:362650/149‑4 (MQ=255)
                   tCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAg                                                                                                                             >  6:195544/1‑149 (MQ=255)
                     aCGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGt                                                                                                                           <  1:334887/149‑1 (MQ=255)
                      cGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTa                                                                                                                          <  3:401774/149‑1 (MQ=255)
                       ggTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTAc                                                                                                                         >  7:353140/1‑149 (MQ=255)
                       ggTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCAGACGTGGTATCCACGTCCGGtggc                                                                                                                                 <  3:102447/141‑4 (MQ=255)
                       ggTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCAGACGTGGTATCCACGTCCGGtggc                                                                                                                                 >  4:102447/1‑138 (MQ=255)
                               atCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTTGGGTGAAAAGCCCGTGGGGGTCAGGCCCTGGGTCCCGGCCCCCGGACGTGGATACCACTTGTGGTAGGGCAGGTACTCGcc                                                                                                                    >  1:353850/1‑149 (MQ=255)
                               atCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTCCTCGCCg                                                                                                                   >  3:93784/1‑147 (MQ=255)
                                   aGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCa                                                                                                             >  3:102441/1‑149 (MQ=255)
                                                  ccTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGc                                                                                               >  4:374105/1‑148 (MQ=255)
                                                   cTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGcca                                                                                             >  3:231729/1‑149 (MQ=255)
                                                   cTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGcca                                                                                             >  4:393756/1‑149 (MQ=255)
                                                   cTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGcca                                                                                             >  8:214003/1‑149 (MQ=255)
                                                      gAAGCGTTTTTGCAACTGCGCCGCCCCGAAGTTTTCCACCGTCTT‑‑GTG‑CCAGCCCGGGGGGGTCAGGCCCTGTGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGccacca                                                                                          <  5:71388/149‑1 (MQ=255)
                                                      gAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGccac                                                                                            <  7:137443/147‑1 (MQ=255)
                                                        aGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGt                                                                                        <  3:184413/149‑1 (MQ=255)
                                                                 gCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCAcgtc                                                                                <  7:214003/148‑1 (MQ=255)
                                                                   aaCTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTtcgtcg                                                                                                 >  3:194370/1‑129 (MQ=255)
                                                                   aaCTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTtcgtcg                                                                                                 <  4:194370/129‑1 (MQ=255)
                                                                    aCTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTAc                                                                            >  5:385875/1‑149 (MQ=255)
                                                                        cgccgcCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGTGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTc                                                                          <  7:321564/147‑1 (MQ=255)
                                                                        cgccgcCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTc                                                                          <  3:151528/147‑1 (MQ=255)
                                                                        cgccgcCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCgg                                                                        >  5:205665/1‑149 (MQ=255)
                                                                        cgccgcCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCg                                                                         <  6:102963/148‑1 (MQ=255)
                                                                         gccgccCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGc                                                                       <  7:196516/149‑1 (MQ=255)
                                                                          ccgccCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCa                                                                      <  3:393756/149‑1 (MQ=255)
                                                                             ccccGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGccacca                                                                                          <  7:78339/126‑1 (MQ=255)
                                                                             ccacGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCAGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGccacca                                                                                          >  8:78339/4‑126 (MQ=255)
                                                                               ccGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTTCTAGGCAGTCTg                                                                 >  6:85372/1‑149 (MQ=255)
                                                                               ccGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTg                                                                 >  3:382330/1‑149 (MQ=255)
                                                                                cGAAGGTTTCCACCGTCTT‑‑CTG‑CCAGCCCGAGGGGCTCAGGCCCAGGATACCGGCCAACGCACGAGGAAACCACGTCTGGAAGGGCAGGTACTCGCCGAAGACACCCCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCtcg                                                                >  1:315538/1‑147 (MQ=255)
                                                                                  aaGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGt                                                              <  4:102441/149‑1 (MQ=255)
                                                                                        tCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAg                                                        >  5:321437/1‑149 (MQ=255)
                                                                                          cACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAg                                                        <  8:254538/147‑1 (MQ=255)
                                                                                          cACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAg                                                        >  7:254538/1‑147 (MQ=255)
                                                                                                cTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGt                                                >  5:156873/1‑149 (MQ=255)
                                                                                                         ccAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGcc                                           <  6:205665/148‑1 (MQ=255)
                                                                                                          cAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTg                                         >  6:395554/1‑149 (MQ=255)
                                                                                                           aGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTgg                                        <  2:337026/149‑1 (MQ=255)
                                                                                                             cccGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGgc                                      >  5:381180/1‑149 (MQ=255)
                                                                                                                gTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCt                                   <  6:381180/149‑1 (MQ=255)
                                                                                                                gTGGGGGTCAGACCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCt                                   >  2:361132/1‑149 (MQ=255)
                                                                                                                   ggggTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTgcg                                <  8:353140/149‑1 (MQ=255)
                                                                                                                      gTCAGGCCCTGGCTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCCCCCAAGTCACCGCGTTCGTCGGCCACCAGAACCACGTCGAACACGGCAGTCTGCGTGAACAGCTGCATGTCGACCTGGGCCCTgctgcg                             >  2:432921/1‑149 (MQ=255)
                                                                                                                       tCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACACGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGgcgc                            >  2:113660/1‑149 (MQ=255)
                                                                                                                           gCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGt                        <  2:353850/149‑1 (MQ=255)
                                                                                                                           gCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGt                        >  1:328930/1‑149 (MQ=255)
                                                                                                                            cccTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTc                       <  1:214432/149‑1 (MQ=255)
                                                                                                                             ccTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGACAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTgc                                 >  7:195921/1‑138 (MQ=255)
                                                                                                                             ccTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGACAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTgc                                 <  8:195921/138‑1 (MQ=255)
                                                                                                                              cTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGt                     >  1:27561/1‑149 (MQ=255)
                                                                                                                                 ggTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGt                  >  1:296512/1‑149 (MQ=255)
                                                                                                                                   tACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCg                <  5:195544/149‑1 (MQ=255)
                                                                                                                                              agACGTGGTATCCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGt     >  6:270995/2‑149 (MQ=255)
                                                                                                                                               gACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGtt    >  5:315110/1‑149 (MQ=255)
                                                                                                                                                 cGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGtttt  <  3:374105/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
AGCCATCCAGGCGGCGAAGTCACGGTCATTCATCCAGCGCCCGGCCTGGGCCTCGAAGCGTTTTTGCAACTGCGCCGCCCCGAAGGTTTCCACCGTCTT‑‑GTG‑CCAGCCCGTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTT  >  NC_002947/3057651‑3057941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑