Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,118,002 T→C 10.2% T194A (ACC→GCC)  cfa ← cyclopropane‑fatty‑acyl‑phospholipid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,118,0020TC10.2% 98.5 / 2.7 39T194A (ACC→GCC) cfacyclopropane‑fatty‑acyl‑phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base T (18/17);  new base C (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ATCATCTCGATCGAAACCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTGGGCCTGCTCCAGTGTTTGCCC  >  NC_002947/3117857‑3118149
                                                                                                                                                 |                                                                                                                                                   
atcatcTCGATCGAAACCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGAc                                                                                                                                                  <  7:342625/149‑1 (MQ=255)
    tctcGATCGAAACCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTg                                                                                                                                              >  1:263113/1‑149 (MQ=255)
             aaaCCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTaa                                                                                                                                     >  6:330863/1‑149 (MQ=255)
             aaaCCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGACGTaa                                                                                                                                     >  5:312246/1‑149 (MQ=255)
                         cGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTg                                                                                                                               <  1:319039/143‑1 (MQ=255)
                         cGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTg                                                                                                                               >  2:319039/1‑143 (MQ=255)
                         cGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCg                                                                                                                         >  1:175581/1‑149 (MQ=255)
                                gCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAg                                                                                                                  >  1:340396/1‑149 (MQ=255)
                                   ttGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAga                                                                                                                 >  6:194382/1‑146 (MQ=255)
                                              ggTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAg                                                                                                    >  2:392058/1‑149 (MQ=255)
                                               gTAGTCATCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTAGGAGAGCGTCGTGGTGGTGACCCTGCAGCCCTAACGGGTGGCTGCGTGGATAGCGAGCCTGCCCCAGc                                                                                                   >  1:252800/1‑149 (MQ=255)
                                                   tCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCt                                                                                               <  4:136137/149‑1 (MQ=255)
                                                                cTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCcagc                                                                                  <  6:429129/149‑1 (MQ=255)
                                                                    cacaCGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGt                                                                              <  5:330863/149‑1 (MQ=255)
                                                                       acGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCACAAGCCGCTGCCAATTTCCAGAAGGTGAt                                                                           >  4:110201/1‑149 (MQ=255)
                                                                             ccccagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGATCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCa                                                                      <  5:137783/148‑1 (MQ=255)
                                                                              cccagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTcc                                                                                     <  4:58564/132‑1 (MQ=255)
                                                                              cccagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTcc                                                                                     >  3:58564/1‑132 (MQ=255)
                                                                              cccagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCa                                                                      >  7:247284/1‑147 (MQ=255)
                                                                              cccagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCa                                                                      <  8:247284/147‑1 (MQ=255)
                                                                                cagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGc                                                                                                   >  4:13487/1‑116 (MQ=255)
                                                                                cagccccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGc                                                                                                   <  3:13487/116‑1 (MQ=255)
                                                                                     ccaAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGt                                                             <  2:45932/149‑1 (MQ=255)
                                                                                         ggCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGa                                                         <  8:110597/149‑1 (MQ=255)
                                                                                         ggCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGa                                                         <  5:67775/149‑1 (MQ=255)
                                                                                            cTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTg                                                                             >  2:32995/1‑126 (MQ=255)
                                                                                            cTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTg                                                                             <  1:32995/126‑1 (MQ=255)
                                                                                                 cgcgCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGGCGTAACTGGTGGCTGCGTGGATAGCGAGGCTGCCCCATCCGCTGCGAATTTCCATCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTgg                                                 <  6:312246/149‑1 (MQ=255)
                                                                                                 cgcgCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTgg                                                 <  6:89677/149‑1 (MQ=255)
                                                                                                    gcTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTgg                                                 >  7:191978/1‑146 (MQ=255)
                                                                                                           ggTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGCGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCt                                       >  6:324809/1‑149 (MQ=255)
                                                                                                           ggTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGCGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCt                                       >  6:330739/1‑149 (MQ=255)
                                                                                                                     tACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTTGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTtgtg                             >  4:244658/1‑149 (MQ=255)
                                                                                                                     tACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTtg                               <  3:110201/147‑1 (MQ=255)
                                                                                                                        tGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTc                                      <  3:72390/137‑1 (MQ=255)
                                                                                                                        tGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTc                                      >  4:72390/1‑137 (MQ=255)
                                                                                                                             cTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTggg                     >  8:67347/1‑149 (MQ=255)
                                                                                                                             cTCGGAGAGCGTCGTGGTGGCGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTggg                     <  5:330739/149‑1 (MQ=255)
                                                                                                                             cTCGGAGAGCGTCGTGGTGGCGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTggg                     <  5:324809/149‑1 (MQ=255)
                                                                                                                                              tggtgACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTGGGCCTGCTCCAGTGTTTGc    >  1:26448/1‑149 (MQ=255)
                                                                                                                                                gtgACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTGGGCCTGCTCCAGTGTTTGccc  >  2:200327/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATCATCTCGATCGAAACCAGTTTGTCGAACGTGCCTTGAAGGTCGCGGTAGTCTTCGCGGAGCACTTGCACACGCTGCCCCAGCCCCAAGGCCTTGACGCGCTCCAGGGTATGGCTGTACTGCGCCTCGGAGAGCGTCGTGGTGGTGACCCTGCAGCCGTAACGGGTGGCTGCGTGGATAGCGAGGCTGCCCCAGCCGCTGCCAATTTCCAGCAGGTGATCGTCAGGGCTCAGTTCGAGCTTCTGGCAGATGCGCTCCAGCTTGTGCAACTGGGCCTGCTCCAGTGTTTGCCC  >  NC_002947/3117857‑3118149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑