Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,790 G→C 7.9% intergenic (+11/‑40) PP_2785 → / → PP_2786 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,7900GC 7.9% 141.2 / 2.3 48intergenic (+11/‑40)PP_2785/PP_2786hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/27);  new base C (2/2);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GGGATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCC  >  NC_002947/3173642‑3173934
                                                                                                                                                    |                                                                                                                                                  
gggATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtg                                                                                                                                                    >  3:409508/1‑149 (MQ=255)
         aCCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGc                                                                                                                                            >  2:354804/1‑148 (MQ=255)
         aCCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtgg                                                                                                                                            <  3:161637/148‑2 (MQ=255)
          ccTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGa                                                                                                                                          >  2:344092/1‑149 (MQ=255)
          ccTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGACAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtgttc                                                                                                                                          <  2:374424/149‑4 (MQ=255)
             aCAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCAAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAAc                                                                                                                                        >  1:206152/1‑148 (MQ=255)
              cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAAc                                                                                                                                        <  2:72107/147‑1 (MQ=255)
              cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAAc                                                                                                                                        <  2:301145/147‑1 (MQ=255)
                  ccGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTc                                                                                                                                   >  1:34483/1‑148 (MQ=255)
                    ggTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCaaa                                                                                                                                <  7:221645/149‑1 (MQ=255)
                          cTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATg                                                                                                                           >  8:63430/1‑148 (MQ=255)
                          cTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGa                                                                                                                          <  3:406500/149‑1 (MQ=255)
                             gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaaaa                                                                                                                       <  4:397389/149‑1 (MQ=255)
                             gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaaaa                                                                                                                       <  6:299079/149‑1 (MQ=255)
                               tCAAGTTGGGGCTCGAAGTCGATGTGATATTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                      >  5:209874/1‑148 (MQ=255)
                                 aaGTTGTGGCTCGAAGTCGTTGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACATCCGCGATGAGGTGCGCTTGTCGGCCGGTTATCTGTTCTGACCTCACCTGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGaa                                                                                                                   <  7:85482/149‑1 (MQ=255)
                                    ttGGGGCTCGTAGTCGATGTGTTCTTCGTCAAGCCCGACCGCCAGTTCGGTTCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATc                                                                                                                <  6:328852/149‑1 (MQ=255)
                                       gggCTCGAAGTCGATGTGTTCTTCGGCACGCCCGACCGCCAGTTCGGTGCGTCCGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAATTCGc                                                                                                              >  8:352151/1‑148 (MQ=255)
                                              actgcGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCTATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGc                                                                                                     <  8:344034/145‑1 (MQ=255)
                                              aaGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGc                                                                                                     >  3:249744/1‑149 (MQ=255)
                                                   gATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATc                                                                                                <  1:106587/149‑1 (MQ=255)
                                                       tgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACTCCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAgg                                                                                                                     <  5:188325/125‑1 (MQ=255)
                                                       tgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTCCGGTGTGCGAAACACTCAAACAATCAAAAgg                                                                                                                     >  6:188325/1‑125 (MQ=255)
                                                               ggCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCt                                                                                     <  2:34483/148‑1 (MQ=255)
                                                                gCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGCAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGa                                                                                   <  4:205774/149‑1 (MQ=255)
                                                                  aaGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCGATCTTCAAGACCCTGACt                                                                                 >  4:101130/1‑149 (MQ=255)
                                                                       cGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAg                                                                           >  2:101955/1‑149 (MQ=255)
                                                                             ccAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCAct                                                                     >  6:99757/1‑149 (MQ=255)
                                                                              cAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCActg                                                                    <  2:229186/149‑1 (MQ=255)
                                                                                        cGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAgc                                                           <  4:409508/148‑1 (MQ=255)
                                                                                                aaCCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTgcc                                                  <  5:4605/149‑1 (MQ=255)
                                                                                                 aCCGCGATCATGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCTCACTGCTGTTGAGCGCCGCTgccg                                                 <  3:101130/149‑1 (MQ=255)
                                                                                                 aCCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTgccg                                                 <  1:3464/149‑1 (MQ=255)
                                                                                                   cgcgATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGACCATCTTAAAGACCCTGAAT‑GTAAGCGCACTGATGTTGAGAGCAGCTgccgc                                                >  6:244668/1‑148 (MQ=255)
                                                                                                       aTCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATgg                                           <  6:209874/149‑1 (MQ=255)
                                                                                                        tCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATgg                                           >  3:11769/1‑148 (MQ=255)
                                                                                                        tCAGGAGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGc                                          <  5:244668/149‑1 (MQ=255)
                                                                                                         cagcaccgcTTGTCCGCCGGATATCTGTTCTGACCACACCCGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCAA‑TGTTCCCCAACTTCAACACCCTGACT‑CAAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATgg                                           >  2:167240/7‑147 (MQ=255)
                                                                                                          aGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCa                                         <  1:344092/148‑1 (MQ=255)
                                                                                                            gTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCt                                      >  5:341173/1‑149 (MQ=255)
                                                                                                              gcgcTTGTCCGCCGGTTATCTGTTCTGAGCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTg                                     >  1:244347/1‑148 (MQ=255)
                                                                                                                    gTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACTCGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAaca                                <  1:101955/148‑1 (MQ=255)
                                                                                                                        gccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgcacg                          >  7:25681/1‑149 (MQ=255)
                                                                                                                          cgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGc                        <  2:206152/149‑1 (MQ=255)
                                                                                                                               aTCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCcgac                    <  4:249744/148‑1 (MQ=255)
                                                                                                                                    ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcat               <  2:244347/148‑1 (MQ=255)
                                                                                                                                      cTGTCCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAATGAAATCGCCATTCTTGCCCATCTTCAAGAGTTTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcatc              <  1:354804/148‑1 (MQ=255)
                                                                                                                                       ttcgcacaCCGCACCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCAtt           >  3:65962/5‑149 (MQ=255)
                                                                                                                                              acCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAgg    <  5:99757/149‑1 (MQ=255)
                                                                                                                                                cggtgcggtgTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGcc  >  4:413191/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGGATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCA‑TGTTGCCCATCTTCAAGACCCTGACT‑GTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCC  >  NC_002947/3173642‑3173934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑