Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,201,679 T→A 6.8% V336E (GTA→GAA) ‡ PP_2808 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,201,6790TA 6.8% 208.9 / 2.6 68V336E (GTA→GAA) ‡PP_2808oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base A (3/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATCATCACGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCA  >  NC_002947/3201532‑3201826
                                                                                                                                                   |                                                                                                                                                   
ggATCATCACGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTa                                                                                                                                                    >  8:2381/1‑149 (MQ=255)
      tcaCGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTgcgc                                                                                                                                              >  1:127359/1‑149 (MQ=255)
      tcaCGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTgcgc                                                                                                                                              <  7:338399/149‑1 (MQ=255)
      tcaCGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTCGCGCAGTACCTgcgc                                                                                                                                              >  8:167475/1‑149 (MQ=255)
          gccgcACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGACAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGCCCAGGCCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAgc                                                                                                                                          >  3:84037/1‑149 (MQ=255)
            cgcACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAgcgc                                                                                                                                        >  5:192205/1‑149 (MQ=255)
             gcACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAgcgc                                                                                                                                        >  3:83252/1‑148 (MQ=255)
             gcACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTCGCGCAGTACCTgcgccaatgcc                                                                                                                                       <  7:167475/149‑8 (MQ=255)
                 tACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATcc                                                                                                                                   >  7:1222/1‑149 (MQ=255)
                    acCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCgg                                                                                                                                 >  3:284907/1‑148 (MQ=255)
                     ccATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCa                                                                                                                               >  8:102579/1‑149 (MQ=255)
                        tGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTg                                                                                                                            >  6:294911/1‑149 (MQ=255)
                        tGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTg                                                                                                                            <  1:380605/149‑1 (MQ=255)
                        tGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTg                                                                                                                            >  2:380605/1‑149 (MQ=255)
                          gagaGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGc                                                                                                                           <  5:38410/148‑1 (MQ=255)
                              gCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGTTATCCGGCATTGCAAGGc                                                                                                                      <  7:355956/149‑1 (MQ=255)
                              gCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGc                                                                                                                      <  2:303423/149‑1 (MQ=255)
                               ccACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGcc                                                                                                                     >  7:78400/1‑149 (MQ=255)
                                cACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCt                                                                                                                    >  1:388639/1‑149 (MQ=255)
                                  ccGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTg                                                                                                                  <  7:52310/149‑1 (MQ=255)
                                   cGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTgg                                                                                                                 <  7:2381/149‑1 (MQ=255)
                                        gACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAgc                                                                                                                                          <  7:47656/119‑1 (MQ=255)
                                        gACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAgc                                                                                                                                          >  8:47656/1‑119 (MQ=255)
                                           ggcacacggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAg                                                                                                          >  4:241517/1‑148 (MQ=255)
                                             cacacggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACTGTTCGAAGACGGCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCAc                                                                                                       <  7:56515/149‑1 (MQ=255)
                                                acggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActgc                                                                                                    >  4:123320/1‑149 (MQ=255)
                                                acggcacGAGGCTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCACAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActgc                                                                                                    >  2:297784/1‑149 (MQ=255)
                                                 cggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTc                                                                                                             >  7:275860/1‑139 (MQ=255)
                                                 cggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTc                                                                                                             <  8:275860/139‑1 (MQ=255)
                                                 cggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTc                                                                                                             <  8:287259/139‑1 (MQ=255)
                                                 cggcacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTc                                                                                                             >  7:287259/1‑139 (MQ=255)
                                                    cacGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActgctg                                                                                                  >  7:82720/1‑147 (MQ=255)
                                                          ggTGTTGGCCGTCATGAAGCTAGGTTATGCCTACGGTTCTAAGACGACCAATGTCCCGGACCAGGGCGCCTATGAGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGt                                                                                           <  3:319367/148‑1 (MQ=255)
                                                           gtgtTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGtt                                                                                          <  6:192205/148‑1 (MQ=255)
                                                                 aCCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAg                                                                                   >  3:335296/1‑149 (MQ=255)
                                                                  ccGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGt                                                                                  >  1:233940/1‑149 (MQ=255)
                                                                     tCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGTACCTgcgccaa                                                                                                                                           <  6:224234/89‑4 (MQ=255)
                                                                     tCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGTACCTgcgccaa                                                                                                                                           >  5:224234/1‑86 (MQ=255)
                                                                     tCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActg                                                                                                     >  6:209911/1‑127 (MQ=255)
                                                                     tCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActg                                                                                                     <  5:209911/127‑1 (MQ=255)
                                                                      cAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGgcg                                                                              >  5:37441/1‑149 (MQ=255)
                                                                         gaagCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGcc                                                                            >  2:35096/1‑148 (MQ=255)
                                                                                 gTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGAGCAGGGCGCCTATGCGGCTTTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACttt                                                                   <  4:84037/149‑1 (MQ=255)
                                                                                  ttATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCTCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTc                                                                  <  1:297784/149‑1 (MQ=255)
                                                                                      gCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGcc                                                              >  6:386423/1‑149 (MQ=255)
                                                                                        cTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCgg                                                            <  2:186284/149‑1 (MQ=255)
                                                                                         tACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActgct                                                                                                   >  1:135205/1‑109 (MQ=255)
                                                                                         tACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCActgct                                                                                                   <  2:135205/109‑1 (MQ=255)
                                                                                                 gAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTg                                                   <  2:388639/149‑1 (MQ=255)
                                                                                                       gCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGt                                                    >  5:198298/1‑142 (MQ=255)
                                                                                                       gCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGt                                                    <  6:198298/142‑1 (MQ=255)
                                                                                                               tCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGaaa                                     >  4:3889/1‑149 (MQ=255)
                                                                                                                 ccGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGaaa                                     >  8:257979/1‑147 (MQ=255)
                                                                                                                  cGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGaaa                                     >  2:255260/1‑146 (MQ=255)
                                                                                                                        aGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACAtt                            <  3:241517/149‑1 (MQ=255)
                                                                                                                          ggCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTta                          <  5:386423/149‑1 (MQ=255)
                                                                                                                           gcgcCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTtac                         <  2:152690/149‑1 (MQ=255)
                                                                                                                           gcgcCAATGCCGCATAGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTtac                         >  3:128709/1‑149 (MQ=255)
                                                                                                                             gcCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTtacta                       >  2:84763/1‑149 (MQ=255)
                                                                                                                                   gCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCAc                 >  6:279698/1‑149 (MQ=255)
                                                                                                                                       cATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGgc             <  6:37441/149‑1 (MQ=255)
                                                                                                                                       cATTGGCGCAGGAACTCCGCGATCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCgg              >  4:248131/1‑148 (MQ=255)
                                                                                                                                              gcAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCgg      <  2:127359/149‑1 (MQ=255)
                                                                                                                                              gcAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCgg      <  7:102579/149‑1 (MQ=255)
                                                                                                                                              gcAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGGGGCGCCTACTACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCgg      <  3:17656/149‑1 (MQ=255)
                                                                                                                                               cAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCggg     <  5:294911/149‑1 (MQ=255)
                                                                                                                                               cAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCggg     <  1:35096/149‑1 (MQ=255)
                                                                                                                                                  gTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCa  >  6:95211/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGATCATCACGCCGCACTACACCATGGAGAGCCACCGCCTGACGGCACACGGCACGATGGTGTTGACCGTCAAGAAGCTAGGTTATGCCTACGGTTCGAAGACGCCCAATGTCCCGGACCAGGGCGCCTATGCGGCATTGGCGCAGGTACTGCGCGAGCGCTATCCGGCATTGCAAGGCCTTGGCGTCAAGCACTGCTGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCA  >  NC_002947/3201532‑3201826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑