Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,292,859 G→C 5.1% G715R (GGC→CGC)  PP_2893 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,292,8590GC 5.1% 255.6 / 3.3 79G715R (GGC→CGC) PP_2893hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base C (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGACAAAGGCC  >  NC_002947/3292712‑3293006
                                                                                                                                                    |                                                                                                                                                    
gcgcCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTggg                                                                                                                                                     <  4:346640/149‑1 (MQ=255)
 cgcctgcacgcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGc                                                                                                                                                    <  2:347767/149‑1 (MQ=255)
  gcctgcacgcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTggg                                                                                                                                                     >  5:371883/1‑147 (MQ=255)
      gcacgcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCt                                                                                                                                               >  7:75192/1‑149 (MQ=255)
       cacgcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGcc                                                                                                                                                <  7:200467/147‑1 (MQ=255)
         cgcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGc                                                                                                                                            <  8:75192/149‑1 (MQ=255)
          gcctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGcc                                                                                                                                           <  7:219188/149‑1 (MQ=255)
            ctgcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGcc                                                                                                                                           >  4:313822/1‑147 (MQ=255)
              gcaGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTgg                                                                                                                                             <  3:109245/143‑1 (MQ=255)
               caGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAg                                                                                                                                        <  3:416995/147‑1 (MQ=255)
                  cGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCt                                                                                                                                   <  1:115762/149‑1 (MQ=255)
                  cGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCCCTGGGCAGCCTGGCCCAGTGGCt                                                                                                                                   >  8:291415/1‑149 (MQ=255)
                    gCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGc                                                                                                                                 <  8:217751/149‑1 (MQ=255)
                    gCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGc                                                                                                                                 <  2:268188/149‑1 (MQ=255)
                    gCTGGCCAGCGACCAGCTCGACTTCGGGGCCCACTGCTGGCGCTACGGCCAGTCAAG‑CCTGTGTGGCGACGACCAGAGACTGGCCCCCGAACCGCGCCTGCGGTACCACCTCATGCATTTACCGCTTGGCAGCCTGGACCAGTGGCTGc                                                                                                                                 <  8:292320/149‑1 (MQ=255)
                     cTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGAGCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGc                                                                                                                                 >  1:119565/1‑149 (MQ=255)
                          cagcggccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAg                                                                                                                            >  7:80019/1‑148 (MQ=255)
                          cagcggccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAg                                                                                                                            >  2:367535/1‑148 (MQ=255)
                            gcggccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑Ac                                                                                                                        <  2:46463/149‑1 (MQ=255)
                               gccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACttt                                                                                                                     >  1:109972/1‑149 (MQ=255)
                               gccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGCGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACttt                                                                                                                     >  8:318214/1‑149 (MQ=255)
                               gccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGCGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACttt                                                                                                                     >  8:324264/1‑149 (MQ=255)
                                ccagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTg                                                                                                                    <  4:387457/149‑1 (MQ=255)
                                 cagcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGc                                                                                                                   <  5:325112/149‑1 (MQ=255)
                                   gcTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCt                                                                                                                 <  2:119565/149‑1 (MQ=255)
                                    cTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTg                                                                                                                >  7:298770/1‑149 (MQ=255)
                                      cGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGc                                                                                                              <  5:39711/149‑1 (MQ=255)
                                          ttCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTCCCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAgg                                                                                                          >  6:137834/1‑149 (MQ=255)
                                            cGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGc                                                                                                         <  1:367535/148‑1 (MQ=255)
                                            cGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAACAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAgg                                                                                                          <  3:14294/147‑1 (MQ=255)
                                              gggCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTg                                                                                                      <  8:330619/149‑1 (MQ=255)
                                                        ctgGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGcc                                                                                             >  4:160613/1‑148 (MQ=255)
                                                           gcgcTCCGGCCAGGCAAG‑ACTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCTCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGa                                                                                           <  6:18603/147‑1 (MQ=255)
                                                             gcTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCg                                                                                            <  7:260386/144‑1 (MQ=255)
                                                             gcTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCg                                                                                            >  8:260386/1‑144 (MQ=255)
                                                             gcTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACAt                                                                                        >  1:106967/1‑148 (MQ=255)
                                                                 cGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAAcc                                                                                   >  2:87264/1‑149 (MQ=255)
                                                                        gCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGGTACCACGTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑CCTTTGCCTGGCAAGGCATGCTCAATGCCGACATCAACCTGGCCaa                                                                            >  6:311312/1‑148 (MQ=255)
                                                                        gCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGt                                                                                                                                       >  7:383160/1‑91 (MQ=255)
                                                                        gCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGt                                                                                                                                       <  8:383160/91‑1 (MQ=255)
                                                                          aaG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGACCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATcc                                                                          >  2:46026/1‑149 (MQ=255)
                                                                           aG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGCGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATccc                                                                         <  7:324264/149‑1 (MQ=255)
                                                                           aG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGCGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATccc                                                                         <  7:318214/149‑1 (MQ=255)
                                                                            g‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCg                                                                        <  2:106967/149‑1 (MQ=255)
                                                                                    tgGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATc                                                                                       <  6:27110/127‑1 (MQ=255)
                                                                                    tgGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATc                                                                                       >  5:27110/1‑127 (MQ=255)
                                                                                           gacCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCgg                                                                >  7:103797/1‑143 (MQ=255)
                                                                                           gacCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCgg                                                                <  8:103797/143‑1 (MQ=255)
                                                                                           gacCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGcccc                                                            >  4:423707/1‑147 (MQ=255)
                                                                                           gacCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCaa                                                          <  8:80019/149‑1 (MQ=255)
                                                                                              cAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAggg                                                       <  8:298770/149‑1 (MQ=255)
                                                                                               aGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATc                                                                                       >  6:352971/1‑116 (MQ=255)
                                                                                               aGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATc                                                                                       <  5:352971/116‑1 (MQ=255)
                                                                                                       gCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCg                                               <  6:371883/148‑1 (MQ=255)
                                                                                                        cccccGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTg                                             <  5:311312/149‑1 (MQ=255)
                                                                                                         ccccGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTgg                                            >  7:388542/1‑149 (MQ=255)
                                                                                                          cccGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGt                                           <  3:426266/149‑1 (MQ=255)
                                                                                                           ccGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTc                                          >  5:173583/1‑149 (MQ=255)
                                                                                                           ccGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTc                                          >  4:428728/1‑149 (MQ=255)
                                                                                                             gAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGa                                        <  1:727/149‑1 (MQ=255)
                                                                                                               aCCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATg                                      >  8:10200/1‑149 (MQ=255)
                                                                                                                ccGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGc                                     >  6:378011/1‑149 (MQ=255)
                                                                                                                      cTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTgg                               <  2:109972/149‑1 (MQ=255)
                                                                                                                        gcgcTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGGTACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTggc                              >  1:119384/1‑149 (MQ=255)
                                                                                                                         cgcTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTggc                              <  3:313822/147‑1 (MQ=255)
                                                                                                                          gcTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTggcgg                            >  1:165880/1‑148 (MQ=255)
                                                                                                                            tACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCAc                         >  8:201104/1‑149 (MQ=255)
                                                                                                                            tACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCAc                         >  4:1046/1‑149 (MQ=255)
                                                                                                                              ccaccTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCAccc                       >  2:242130/1‑149 (MQ=255)
                                                                                                                               ccccTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCATGGCAAGGACAGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGCCACTGTCGTGGTCGATGCCAGTGGCGGCACCCt                      >  3:406402/3‑149 (MQ=255)
                                                                                                                                 ccTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCtgc                    <  7:291415/149‑1 (MQ=255)
                                                                                                                                 ccTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCtgc                    >  3:20489/1‑149 (MQ=255)
                                                                                                                                  cTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGc                                                                  >  7:292531/1‑102 (MQ=255)
                                                                                                                                  cTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGc                                                                  <  8:292531/102‑1 (MQ=255)
                                                                                                                                     aaGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCa                                   >  4:27789/1‑130 (MQ=255)
                                                                                                                                     aaGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCa                                   <  3:27789/130‑1 (MQ=255)
                                                                                                                                         aGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCtgcgcgtgcgc            <  3:160613/149‑1 (MQ=255)
                                                                                                                                         aGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCtgcgcgtgcgc            <  7:10200/149‑1 (MQ=255)
                                                                                                                                           ttCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGa          <  3:423707/149‑1 (MQ=255)
                                                                                                                                               cGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCGCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGACa        >  6:319039/1‑147 (MQ=255)
                                                                                                                                                 cTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGACAAAgg    >  3:118165/1‑149 (MQ=255)
                                                                                                                                                   gggCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGACAAAGGcc  >  8:346730/1‑149 (MQ=255)
                                                                                                                                                    ggCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGt                                           <  5:327350/107‑1 (MQ=255)
                                                                                                                                                    ggCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGt                                           >  6:327350/1‑107 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAG‑CCTGTGTGGCGACGACCAGCGACTGGCCCCCGAACCGCGCCTGCGCTACCACCTCAAGCAGTTCCCGCTGGGCAGCCTGGCCCAGTGGCTGCCGAAGG‑ACTTTGCCTGGCAAGGCCTGCTCAATGCCGACATCAACCTGGACATCCCGGCCAGCGGCCCCAAGGGCACTGTCGTGGTCGATGCCAGTGGCGGCACCCTGCGCGTGCGCGACAAAGGCC  >  NC_002947/3292712‑3293006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑