Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,466,058	G→C	6.6%	noncoding (102/119 nt)	PP_mr39 ←	P15 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,466,058	0	G	C	6.6%	242.7 / 5.2	76	noncoding (102/119 nt)	PP_mr39	P15 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (41/30);  new base C (2/3);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

AGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAGGCT  >  NC_002947/3465914‑3466206                                                                                                                                                   |                                                                                                                                                       aGGCGGTAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGa                                                                                                                                                    >  2:55370/1‑149 (MQ=255)     ggcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCgg                                                                                                                                                <  1:420687/149‑1 (MQ=255)     ggcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCgg                                                                                                                                                <  1:427261/149‑1 (MQ=255)           cTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                          <  2:186322/149‑1 (MQ=255)            tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCAGGAGAAAAAAAAAACCCCCGGTCGGTAAGCCGACCGGGGGtt                                                                                                                                           >  1:364209/1‑149 (MQ=255)            tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttt                                                                                                                                          >  5:192526/1‑148 (MQ=255)             ggCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCgggg                                                                                                                                              <  1:193953/143‑1 (MQ=255)             ggCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCgggg                                                                                                                                              >  2:193953/1‑143 (MQ=255)              gCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                                        <  8:184868/148‑1 (MQ=255)              gCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttt                                                                                                                                          <  6:192526/146‑1 (MQ=255)               cccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAg                                                                                                                                       >  2:309562/1‑148 (MQ=255)                ccGAACGGGATTGTCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTa                                                                                                                                     <  4:370313/149‑1 (MQ=255)                ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGTAAGCCGACCGGGGGTTTGAGTa                                                                                                                                     >  8:234560/1‑149 (MQ=255)                 cGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTAt                                                                                                                                    >  2:305999/1‑149 (MQ=255)                    aCGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATtct                                                                                                                                 <  8:89416/149‑1 (MQ=255)                        gATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATg                                                                                                                             >  2:11900/1‑149 (MQ=255)                             gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                        <  6:311526/149‑1 (MQ=255)                              cTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggc                                                                                                                       >  4:308455/1‑149 (MQ=255)                              cTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAACGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggc                                                                                                                       >  3:9326/1‑149 (MQ=255)                               tAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggcg                                                                                                                      >  6:395012/1‑149 (MQ=255)                                aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggcgg                                                                                                                     >  5:263610/1‑149 (MQ=255)                                   tCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGccc                                                                                                                  <  5:124295/149‑1 (MQ=255)                                   tCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAAACCACGGTCGGGAAGCCGACCGGGGGTTTGACTATTCTCATGTTCGGCGGccc                                                                                                                  >  8:85270/1‑149 (MQ=255)                                     gCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCt                                                                                                                <  4:147012/149‑1 (MQ=255)                                       cAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑c                                                                                                              >  7:116251/1‑148 (MQ=255)                                           cGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAgg                                                                                                         <  5:395012/149‑1 (MQ=255)                                             tAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTg                                                                                                       <  6:285687/149‑1 (MQ=255)                                                gtgtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGCCCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGAc                                                                                                    >  6:72748/1‑149 (MQ=255)                                                  gtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGc                                                                                                  <  3:308455/149‑1 (MQ=255)                                                        gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                        >  4:129274/1‑122 (MQ=255)                                                        gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                        >  4:342356/1‑122 (MQ=255)                                                        gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                        <  3:342356/122‑1 (MQ=255)                                                        gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                        <  3:129274/122‑1 (MQ=255)                                                        gtagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGCCGGGAAGCAGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCgg‑gtg                                                                                           >  8:227790/1‑149 (MQ=255)                                                         tagtaATACGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCgg‑gtgg                                                                                          <  7:379340/149‑1 (MQ=255)                                                           gtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCgg‑gtggg                                                                                         >  7:73221/1‑148 (MQ=255)                                                              ataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTAt                                                                                      <  6:263610/148‑1 (MQ=255)                                                               taaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCg                                                                                    >  3:66465/1‑149 (MQ=255)                                                                 aGTGGCGTAGTTCATGAATCTGTCGTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACGGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGgc                                                                                  <  1:55370/149‑1 (MQ=255)                                                                     gCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGt                                                                                                        >  2:376014/1‑124 (MQ=255)                                                                     gCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGt                                                                                                        <  1:376014/124‑1 (MQ=255)                                                                       gTATTTCATTAATCTGTCCTCAAACTGAATCGGTGTTGCTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCTCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAg                                                                            <  2:196479/149‑1 (MQ=255)                                                                       gTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAg                                                                            >  8:118825/1‑149 (MQ=255)                                                                                gAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAAc                                                                   >  8:218058/1‑149 (MQ=255)                                                                                     tGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTa                                                                             >  3:223238/1‑134 (MQ=255)                                                                                     tGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTa                                                                             <  4:223238/134‑1 (MQ=255)                                                                                       tCCTCAAAGTTAATCGGTGTTGGTCAAAGGC‑‑CAGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGTCGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAacac                                                             <  6:35493/148‑1 (MQ=255)                                                                                       tCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCaa                                                                <  8:116251/145‑1 (MQ=255)                                                                                       tCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAAt                                                               <  1:305999/146‑1 (MQ=255)                                                                                         cTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                           >  3:154105/1‑148 (MQ=255)                                                                                          tCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCa                                                          >  5:247856/1‑148 (MQ=255)                                                                                             aaGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                       >  2:5328/1‑148 (MQ=255)                                                                                             aaGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGt                                                      >  2:42570/1‑149 (MQ=255)                                                                                                  aaTCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGGTGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATa                                                  <  1:309562/149‑1 (MQ=255)                                                                                                   aTCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGc                                                >  7:103982/1‑149 (MQ=255)                                                                                                      ggTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                             <  2:364209/149‑1 (MQ=255)                                                                                                       gtgtTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGc                                            <  7:234560/149‑1 (MQ=255)                                                                                                          ttGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGACGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAgg                                         >  1:48603/1‑149 (MQ=255)                                                                                                            ggTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGt                                        <  7:118825/148‑1 (MQ=255)                                                                                                             gTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAAc                                                                   <  8:365435/120‑1 (MQ=255)                                                                                                             gTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAAc                                                                   >  7:365435/1‑120 (MQ=255)                                                                                                             gTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGTTGc                                      >  2:268847/1‑149 (MQ=255)                                                                                                              tCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                      >  3:63966/1‑148 (MQ=255)                                                                                                               cAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCa                                    >  4:84386/1‑149 (MQ=255)                                                                                                                aaaGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCAc                                   >  1:296037/1‑149 (MQ=255)                                                                                                                aaaGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCAc                                   >  6:412765/1‑149 (MQ=255)                                                                                                                  aGGC‑‑CTGAAAAACAAAACCCCCGGCCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCCGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGc                                 <  7:227790/149‑1 (MQ=255)                                                                                                                    gC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAg                               >  6:94852/1‑149 (MQ=255)                                                                                                                    gC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAg                               >  6:183290/1‑149 (MQ=255)                                                                                                                          gAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGAGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                           >  6:416240/1‑149 (MQ=255)                                                                                                                                   aacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCa                  <  7:218058/149‑1 (MQ=255)                                                                                                                                    acCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAg                 >  3:195164/1‑149 (MQ=255)                                                                                                                                       cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTACGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca               >  1:331948/1‑149 (MQ=255)                                                                                                                                           gTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                      >  2:252072/1‑121 (MQ=255)                                                                                                                                           gTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                      <  1:252072/121‑1 (MQ=255)                                                                                                                                                gAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAg     >  7:107047/1‑149 (MQ=255)                                                                                                                                                   gCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAGGCt  <  5:183290/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       AGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGC‑‑CTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCT‑CGAAGGTGGACGCCGG‑GTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAGGCT  >  NC_002947/3465914‑3466206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑