Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,528,908 T→A 9.3% L242Q (CTG→CAG)  dnaEB → error‑prone DNA polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,528,9080TA 9.3% 148.5 / 5.8 54L242Q (CTG→CAG) dnaEBerror‑prone DNA polymerase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base A (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

ACGTGCGTGGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCCGCTGCGCTG  >  NC_002947/3528761‑3529055
                                                                                                                                                    |                                                                                                                                                   
aCGTGCGTGGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACctg                                                                                                                                                    <  3:50391/149‑1 (MQ=255)
    gcgtgGGCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTgg                                                                                                                                                <  8:342578/149‑1 (MQ=255)
      gtgGTCGCCGTGCCTTTCAGGAATGAATGACTGACATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATTGCGACC‑GGCACCTGCGCAGCCAGGAGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGcc                                                                                                                                              >  8:390441/1‑149 (MQ=255)
      gtgGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGcc                                                                                                                                              <  8:94762/149‑1 (MQ=255)
            gCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGAcc                                                                                                                                        >  1:435493/1‑149 (MQ=255)
             ccGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGAccc                                                                                                                                       >  2:234320/1‑149 (MQ=255)
               gTGCCTTGCAGGACTGCTTGTCTGCCATCGGGCAGCATTGCAAGTTGAGCGAGGCCGGGCGCTTTATGTTTGCCAATGGGGAAC‑GGCAACTGCGCAGCGAGGCGCAACTGGCCGAGCTGTTTCGGCTCTTCCTGCTGGGCgattcgctg                                                                                                                                     <  6:1001/149‑8 (MQ=255)
               gTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTg                                                                                                                                     <  5:70998/149‑1 (MQ=255)
                        aGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGcc                                                                                                                            <  6:330947/149‑1 (MQ=255)
                               cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                       <  3:398681/131‑5 (MQ=255)
                               cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                       <  7:46025/131‑5 (MQ=255)
                               cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                       >  8:46025/1‑127 (MQ=255)
                               cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                       >  4:398681/1‑127 (MQ=255)
                               cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCt                                                                                                                     >  8:191890/1‑149 (MQ=255)
                                aTGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTg                                                                                                                    >  4:71042/1‑149 (MQ=255)
                                 tGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGc                                                                                                                   <  4:345771/149‑1 (MQ=255)
                                    cTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAg                                                                                                                <  7:19699/149‑1 (MQ=255)
                                     tGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTCGTCATTGCCCGGCGCTGCCAGt                                                                                                               >  4:299231/1‑149 (MQ=255)
                                       ccATCCGCCAGCATTGCCAGGTGAGCGAGTCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGttt                                                                                                             <  6:354539/149‑1 (MQ=255)
                                        cATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGttt                                                                                                             >  8:233592/1‑148 (MQ=255)
                                             gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAACGGGCCCCTGCGCAGCCAGGCGCAACTGGCCGGGCTGTTTCCGCTCGCCCTCCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCTGTTTGAcgt                                                                                                        >  1:337172/1‑147 (MQ=255)
                                             gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGTCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTg                                                                                                       >  7:52388/1‑149 (MQ=255)
                                             gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGgc                                                                                                                        <  1:23373/132‑1 (MQ=255)
                                             gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGgc                                                                                                                        >  2:23373/1‑132 (MQ=255)
                                             gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTg                                                                                                       <  4:286243/149‑1 (MQ=255)
                                                aGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGc                                                                                                    <  7:323242/149‑1 (MQ=255)
                                                   aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCgaga                                                                                                                                          <  5:194966/108‑1 (MQ=255)
                                                   aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCgaga                                                                                                                                          >  6:194966/1‑108 (MQ=255)
                                                   aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCgaa                                                                                                 >  3:79487/1‑149 (MQ=255)
                                                                  aGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCTGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTAc                                                                                  >  6:155656/1‑149 (MQ=255)
                                                                            cTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAAc                                                                        <  1:117569/149‑1 (MQ=255)
                                                                            cTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAAc                                                                        >  3:153124/1‑149 (MQ=255)
                                                                               tCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTAc                                                                                        <  8:402083/130‑1 (MQ=255)
                                                                               tCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTAc                                                                                        >  7:402083/1‑130 (MQ=255)
                                                                                 tGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTg                                                                   >  7:217163/1‑149 (MQ=255)
                                                                                   tttGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCTAACTGAACTACCAGTACCCACGCGAACTGGt                                                                    <  1:234320/146‑1 (MQ=255)
                                                                                          aTGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCTGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAgggg                                                          <  8:223834/149‑1 (MQ=255)
                                                                                          aTGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAgggg                                                          <  5:289038/149‑1 (MQ=255)
                                                                                           tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAg                                                                                     >  5:162312/1‑121 (MQ=255)
                                                                                           tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAg                                                                                     <  6:162312/121‑1 (MQ=255)
                                                                                           tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGc                                                         <  7:191890/149‑1 (MQ=255)
                                                                                                       aCCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGc                                               >  3:220550/1‑148 (MQ=255)
                                                                                                       aCCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGc                                               >  3:226614/1‑148 (MQ=255)
                                                                                                          tGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAgc                                           >  6:405707/1‑149 (MQ=255)
                                                                                                                 ccAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTgc                                    <  4:79487/149‑1 (MQ=255)
                                                                                                                      cgcAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGaa                               <  4:153124/149‑1 (MQ=255)
                                                                                                                        cAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCTGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAAtt                             <  5:155656/149‑1 (MQ=255)
                                                                                                                              gtcGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGa                       <  8:52388/147‑1 (MQ=255)
                                                                                                                              gCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGc                         >  5:216705/1‑147 (MQ=255)
                                                                                                                                      gTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTGCCAGTACCGCCGCGAAGTGGTGCCCGAGGGGCATCCCCCGGCAAGCTGGCTGCGCGAATTGTGGGAGCAAGGcc               >  8:406812/1‑148 (MQ=255)
                                                                                                                                       tATCCGCTCGACCTGCTGTCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGATCGAACTGAACTACCAGTACCCACGCTAACTGGTGCCCGAGGGGCATACCCCTGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCa               <  7:390441/148‑1 (MQ=255)
                                                                                                                                       cagggtcTCGGCCAGCAGGTCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCAt              >  3:95884/7‑149 (MQ=255)
                                                                                                                                         tCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGc            >  6:20122/1‑149 (MQ=255)
                                                                                                                                                 aCCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCcgctgcgc    >  5:47142/1‑149 (MQ=255)
                                                                                                                                                 aCCTGCTGGCCGAGACCCTGGGCATGGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGGTGGCTGCGCGAATTGTGCGAGCAAGGCAGGCcgctgcgc    <  3:299231/149‑1 (MQ=255)
                                                                                                                                                   ctgctgGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCcgctgcgctg  >  3:82164/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGTGCGTGGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCCGCTGCGCTG  >  NC_002947/3528761‑3529055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑