Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,528,911 T→A 9.3% L243Q (CTG→CAG)  dnaEB → error‑prone DNA polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,528,9110TA 9.3% 150.1 / 6.1 54L243Q (CTG→CAG) dnaEBerror‑prone DNA polymerase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base A (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

GCGTGGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCCGCTGCGCTGGC  >  NC_002947/3528765‑3529057
                                                                                                                                                   |                                                                                                                                                  
gcgtgGGCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTgg                                                                                                                                                  <  8:342578/149‑1 (MQ=255)
  gtgGTCGCCGTGCCTTTCAGGAATGAATGACTGACATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATTGCGACC‑GGCACCTGCGCAGCCAGGAGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGcc                                                                                                                                                >  8:390441/1‑149 (MQ=255)
  gtgGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGcc                                                                                                                                                <  8:94762/149‑1 (MQ=255)
        gCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGAcc                                                                                                                                          >  1:435493/1‑149 (MQ=255)
         ccGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGAccc                                                                                                                                         >  2:234320/1‑149 (MQ=255)
           gTGCCTTGCAGGACTGCTTGTCTGCCATCGGGCAGCATTGCAAGTTGAGCGAGGCCGGGCGCTTTATGTTTGCCAATGGGGAAC‑GGCAACTGCGCAGCGAGGCGCAACTGGCCGAGCTGTTTCGGCTCTTCCTGCTGGGCgattcgctg                                                                                                                                       <  6:1001/149‑8 (MQ=255)
           gTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTg                                                                                                                                       <  5:70998/149‑1 (MQ=255)
                    aGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGcc                                                                                                                              <  6:330947/149‑1 (MQ=255)
                           cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                         >  8:46025/1‑127 (MQ=255)
                           cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                         <  7:46025/131‑5 (MQ=255)
                           cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                         <  3:398681/131‑5 (MQ=255)
                           cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGGCCAGCAGGTCgagcgga                                                                                                                                         >  4:398681/1‑127 (MQ=255)
                           cATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCt                                                                                                                       >  8:191890/1‑149 (MQ=255)
                            aTGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTg                                                                                                                      >  4:71042/1‑149 (MQ=255)
                             tGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGc                                                                                                                     <  4:345771/149‑1 (MQ=255)
                                cTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAg                                                                                                                  <  7:19699/149‑1 (MQ=255)
                                 tGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTCGTCATTGCCCGGCGCTGCCAGt                                                                                                                 >  4:299231/1‑149 (MQ=255)
                                   ccATCCGCCAGCATTGCCAGGTGAGCGAGTCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGttt                                                                                                               <  6:354539/149‑1 (MQ=255)
                                    cATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGttt                                                                                                               >  8:233592/1‑148 (MQ=255)
                                         gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAACGGGCCCCTGCGCAGCCAGGCGCAACTGGCCGGGCTGTTTCCGCTCGCCCTCCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCTGTTTGAcgt                                                                                                          >  1:337172/1‑147 (MQ=255)
                                         gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGTCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTg                                                                                                         >  7:52388/1‑149 (MQ=255)
                                         gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGgc                                                                                                                          <  1:23373/132‑1 (MQ=255)
                                         gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGgc                                                                                                                          >  2:23373/1‑132 (MQ=255)
                                         gCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTg                                                                                                         <  4:286243/149‑1 (MQ=255)
                                            aGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGc                                                                                                      <  7:323242/149‑1 (MQ=255)
                                               aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCgaga                                                                                                                                            <  5:194966/108‑1 (MQ=255)
                                               aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCgaga                                                                                                                                            >  6:194966/1‑108 (MQ=255)
                                               aTTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCgaa                                                                                                   >  3:79487/1‑149 (MQ=255)
                                                              aGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCTGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTAc                                                                                    >  6:155656/1‑149 (MQ=255)
                                                                        cTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAAc                                                                          <  1:117569/149‑1 (MQ=255)
                                                                        cTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAAc                                                                          >  3:153124/1‑149 (MQ=255)
                                                                           tCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTAc                                                                                          >  7:402083/1‑130 (MQ=255)
                                                                           tCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTAc                                                                                          <  8:402083/130‑1 (MQ=255)
                                                                             tGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTg                                                                     >  7:217163/1‑149 (MQ=255)
                                                                               tttGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCTAACTGAACTACCAGTACCCACGCGAACTGGt                                                                      <  1:234320/146‑1 (MQ=255)
                                                                                      aTGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCTGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAgggg                                                            <  8:223834/149‑1 (MQ=255)
                                                                                      aTGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAgggg                                                            <  5:289038/149‑1 (MQ=255)
                                                                                       tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAg                                                                                       <  6:162312/121‑1 (MQ=255)
                                                                                       tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAg                                                                                       >  5:162312/1‑121 (MQ=255)
                                                                                       tGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGc                                                           <  7:191890/149‑1 (MQ=255)
                                                                                                   aCCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGc                                                 >  3:220550/1‑148 (MQ=255)
                                                                                                   aCCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGc                                                 >  3:226614/1‑148 (MQ=255)
                                                                                                      tGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAgc                                             >  6:405707/1‑149 (MQ=255)
                                                                                                             ccAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTgc                                      <  4:79487/149‑1 (MQ=255)
                                                                                                                  cgcAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGaa                                 <  4:153124/149‑1 (MQ=255)
                                                                                                                    cAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCTGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAAtt                               <  5:155656/149‑1 (MQ=255)
                                                                                                                          gtcGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGa                         <  8:52388/147‑1 (MQ=255)
                                                                                                                          gCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGc                           >  5:216705/1‑147 (MQ=255)
                                                                                                                                  gTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTGCCAGTACCGCCGCGAAGTGGTGCCCGAGGGGCATCCCCCGGCAAGCTGGCTGCGCGAATTGTGGGAGCAAGGcc                 >  8:406812/1‑148 (MQ=255)
                                                                                                                                   tATCCGCTCGACCTGCTGTCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGATCGAACTGAACTACCAGTACCCACGCTAACTGGTGCCCGAGGGGCATACCCCTGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCa                 <  7:390441/148‑1 (MQ=255)
                                                                                                                                   cagggtcTCGGCCAGCAGGTCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCAt                >  3:95884/7‑149 (MQ=255)
                                                                                                                                     tCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGc              >  6:20122/1‑149 (MQ=255)
                                                                                                                                             aCCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCcgctgcgc      >  5:47142/1‑149 (MQ=255)
                                                                                                                                             aCCTGCTGGCCGAGACCCTGGGCATGGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGGTGGCTGCGCGAATTGTGCGAGCAAGGCAGGCcgctgcgc      <  3:299231/149‑1 (MQ=255)
                                                                                                                                               ctgctgGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCcgctgcgctg    >  3:82164/1‑149 (MQ=255)
                                                                                                                                                  ctgGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCCGCTGCGCTGGc  <  8:217163/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTGGTCGCCGTGCCTTGCAGGACTGCATGACTGCCATCCGCCAGCATTGCCAGGTGAGCGAGGCCGGGCGCTTTCTGTTTGCCAATGGCGAAC‑GGCACCTGCGCAGCCAGGCGCAACTGGCCGAGCTGTATCCGCTCGACCTGCTGGCCGAGACCCTGGTCATTGCCCGGCGCTGCCAGTTTGACCTGAGCGAACTGAACTACCAGTACCCACGCGAACTGGTGCCCGAGGGGCATACCCCGGCAAGCTGGCTGCGCGAATTGTGCGAGCAAGGCATGCCGCTGCGCTGGC  >  NC_002947/3528765‑3529057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑